Jes Vollertsen

A conceptual ecosystem model of microbial communities in enhanced biological phosphorus removal plants

The microbial populations in 25 full-scale activated sludge wastewater treatment plants with enhanced biological phosphorus removal (EBPR plants) have been intensively studied over several years. Most of the important bacterial groups involved in nitrification, denitrification, biological P removal, fermentation, and hydrolysis have been identified and quantified using quantitative culture-independent molecular methods. Surprisingly, a limited number of core species was present in all plants, constituting on average approx. 80% of the entire communities in the plants, showing that the microbial populations in EBPR plants are rather similar and not very diverse, as sometimes suggested. By focusing on these organisms it is possible to make a comprehensive ecosystem model, where many important aspects in relation to microbial ecosystems and wastewater treatment can be investigated. We have reviewed the current knowledge about these microorganisms with focus on key ecophysiological factors and combined this into a conceptual ecosystem model for EBPR plants. It includes the major pathways of carbon flow with specific organic substances, the dominant populations involved in the transformations, interspecies interactions, and the key factors controlling their presence and activity. We believe that the EBPR process is a perfect model system for studies of microbial ecology in water engineering systems and that this conceptual model can be used for proposing and testing theories based on microbial ecosystem theories, for the development of new and improved quantitative ecosystem models and is beneficial for future design and management of wastewater treatment systems.

The activated sludge ecosystem contains a core community of abundant organisms.

Understanding the microbial ecology of a system requires that the observed population dynamics can be linked to their metabolic functions. However, functional characterization is laborious and the choice of organisms should be prioritized to those that are frequently abundant (core) or transiently abundant, which are therefore putatively make the greatest contribution to carbon turnover in the system. We analyzed the microbial communities in 13 Danish wastewater treatment plants with nutrient removal in consecutive years and a single plant periodically over 6 years, using Illumina sequencing of 16S ribosomal RNA amplicons of the V4 region. The plants contained a core community of 63 abundant genus-level operational taxonomic units (OTUs) that made up 68% of the total reads. A core community consisting of abundant OTUs was also observed within the incoming wastewater to three plants. The net growth rate for individual OTUs was quantified using mass balance, and it was found that 10% of the total reads in the activated sludge were from slow or non-growing OTUs, and that their measured abundance was primarily because of immigration with the wastewater. Transiently abundant organisms were also identified. Among them the genus Nitrotoga (class Betaproteobacteria) was the most abundant putative nitrite oxidizer in a number of activated sludge plants, which challenges previous assumptions that Nitrospira (phylum Nitrospirae) are the primary nitrite-oxidizers in activated sludge systems with nutrient removal.

Corrosion of concrete sewers—The kinetics of hydrogen sulfide oxidation

Hydrogen sulfide absorption and oxidation by corroding concrete surfaces was quantified in a test rig consisting of 6 concrete pipes operated under sewer conditions. The test rig was placed in an underground sewer monitoring station with access to fresh wastewater. Hydrogen sulfide gas was injected into the pipe every 2nd hour to peak concentrations around 1000 ppm. After some months of operation, the hydrogen sulfide became rapidly oxidized by the corroding concrete surfaces. At hydrogen sulfide concentrations of 1000 ppm, oxidation rates as high as 1 mg S m(-2) s(-1) were observed. The oxidation process followed simple nth order kinetics with a process order of 0.45-0.75. Extrapolating the results to gravity sewer systems showed that hydrogen sulfide oxidation by corroding concrete is a fast process compared to the release of hydrogen sulfide from the bulk water, resulting in low gas concentrations compared with equilibrium. Balancing hydrogen sulfide release with hydrogen sulfide oxidation at steady state conditions demonstrated that significant corrosion rates--several millimeters of concrete per year--can potentially occur at hydrogen sulfide gas phase concentrations well below 5-10 ppm. The results obtained in the study advances the knowledge on prediction of sewer concrete corrosion and the extent of odor problems.

Towards a better understanding of sewer exfiltration

This paper gives a full review of the importance of sewer leakage, which has received increased attention throughout the last decades. Despite the intensive interdisciplinary research that has been invested, its magnitude is still unclear and a comprehensive solution for the assessment of sewer exfiltration does not seem to be at hand. However, given that mechanisms of exfiltration and the factors influencing its extent are similar all over the world, it seems possible to develop a generic leakage approach. Several methods for modelling sewer leakage are reviewed and the available measuring techniques are critically evaluated. Based on this evaluation, we suggest a unifying framework to facilitate focused model building. Specifically, we identify open research questions and propose to (i) standardise measurement results to enable better understanding, (ii) perform more long-term experiments under realistic field conditions, and (iii) assess the uncertainty of measurement and model results so that findings are not over-interpreted.

Influence of pipe material and surfaces on sulfide related odor and corrosion in sewers

Hydrogen sulfide oxidation on sewer pipe surfaces was investigated in a pilot scale experimental setup. The experiments were aimed at replicating conditions in a gravity sewer located immediately downstream of a force main where sulfide related concrete corrosion and odor is often observed. During the experiments, hydrogen sulfide gas was injected intermittently into the headspace of partially filled concrete and plastic (PVC and HDPE) sewer pipes in concentrations of approximately 1,000 ppm(v). Between each injection, the hydrogen sulfide concentration was monitored while it decreased because of adsorption and subsequent oxidation on the pipe surfaces. The experiments showed that the rate of hydrogen sulfide oxidation was approximately two orders of magnitude faster on the concrete pipe surfaces than on the plastic pipe surfaces. Removal of the layer of reaction (corrosion) products from the concrete pipes was found to reduce the rate of hydrogen sulfide oxidation significantly. However, the rate of sulfide oxidation was restored to its background level within 10-20 days. A similar treatment had no observable effect on hydrogen sulfide removal in the plastic pipe reactors. The experimental results were used to model hydrogen sulfide oxidation under field conditions. This showed that the gas-phase hydrogen sulfide concentration in concrete sewers would typically amount to a few percent of the equilibrium concentration calculated from Henrys law. In the plastic pipe sewers, significantly higher concentrations were predicted because of the slower adsorption and oxidation kinetics on such surfaces.

Stoichiometric and kinetic model parameters for microbial transformations of suspended solids in combined sewer systems

Biotransformation of wastewater particles with different settling velocities has been investigated based on analysis of long term oxygen uptake rate (OUR) measurements and a conceptual model describing aerobic transformations of organic matter has been used for interpretation. No significant changes in the biodegradability of wastewater particles with different settling velocities were found. In this respect wastewater particles differed from earlier findings for sewer sediments. Methods for determination of model parameters describing aerobic heterotrophic biomass growth and wastewater substrate transformations have been derived and values estimated by adding readily biodegradable substrate to wastewater particles and sewer sediments. The validity of modeling results was subsequently tested. The model was capable of predicting biomass growth for long term measurements correctly and to simulate the OUR satisfactorily, however, peaks in OUR were difficult to simulate, probably because of a simplified description of hydrolysis used in the concept. Generally, it can be recommended to use OUR measurements, interpreted by a conceptual model describing aerobic transformations of organic matter, as a valuable methodology for characterization of wastewater particles as well as suspended sewer sediments in terms of COD-fractions and related biotransformations.

AEROBIC AND ANAEROBIC TRANSFORMATIONS OF SULFIDE IN A SEWER SYSTEM - FIELD STUDY AND MODEL SIMULATIONS

The formation and fate of sulfide in a force main and a downstream-located gravity sewer were investigated in an extensive field study. Sulfide formation in the force main was significant. However, during 14 minutes of transport in the gravity sewer, the sulfide concentration decreased 30%, on average. An application of a conceptual sewer process model for simulating the formation and fate of sulfide was demonstrated. Overall, the model predicted that approximately 90% of the decrease of the sulfide concentration in the gravity sewer was the result of sulfide oxidation and that only a small fraction entered the sewer atmosphere, causing odor and corrosion. Even so, the model predicted concrete corrosion rates of up to 1.2 mm/y in the gravity sewer section.

Modeling of hydrogen sulfide oxidation in concrete corrosion products from sewer pipes.

Abiotic and biotic oxidation of hydrogen sulfide related to concrete corrosion was studied in corrosion products originating from a sewer manhole. The concrete corrosion products were suspended in an acidic solution, mimicking the conditions in the pore water of corroded concrete. The removal of hydrogen sulfide and dissolved oxygen was measured in parallel in the suspension, upon which the suspension was sterilized and the measurement repeated. The results revealed the biotic oxidation to be fast compared with the abiotic oxidation. The stoichiometry of the hydrogen sulfide oxidation was evaluated using the ratio between oxygen and hydrogen sulfide uptake. The ratio for the biotic oxidation pointed in the direction of elemental sulfur being formed as an intermediate in the oxidation of hydrogen sulfide to sulfuric acid. The experimental results were applied to suggest a hypothesis and a mathematical model describing the hydrogen sulfide oxidation pathway in a matrix of corroded concrete.

Growth kinetics of hydrogen sulfide oxidizing bacteria in corroded concrete from sewers

Hydrogen sulfide oxidation by microbes present on concrete surfaces of sewer pipes is a key process in sewer corrosion. The growth of aerobic sulfur oxidizing bacteria from corroded concrete surfaces was studied in a batch reactor. Samples of corrosion products, containing sulfur oxidizing bacteria, were suspended in aqueous solution at pH similar to that of corroded concrete. Hydrogen sulfide was supplied to the reactor to provide the source of reduced sulfur. The removal of hydrogen sulfide and oxygen was monitored. The utilization rates of both hydrogen sulfide and oxygen suggested exponential bacterial growth with median growth rates of 1.25 d(-1) and 1.33 d(-1) as determined from the utilization rates of hydrogen sulfide and oxygen, respectively. Elemental sulfur was found to be the immediate product of the hydrogen sulfide oxidation. When exponential growth had been achieved, the addition of hydrogen sulfide was terminated leading to elemental sulfur oxidation. The ratio of consumed sulfur to consumed oxygen suggested that sulfuric acid was the ultimate oxidation product. To the knowledge of the authors, this is the first study to determine the growth rate of bacteria involved in concrete corrosion with hydrogen sulfide as source of reduced sulfur.

Effects of temperature and dissolved oxygen on hydrolysis of sewer solids

Abstract Effects of temperature and dissolved oxygen (DO) on kinetics of microbial transformation processes of suspended sewer sediment particles, suspended wastewater particles and wastewater were investigated. Microbial activity was estimated by the oxygen uptake rate. The effects were studied under conditions where biomass had not acclimated to changed temperature or DO conditions. Hydrolysis processes limited the availability of organic substrates for the aerobic microbial growth. The effects of temperature were interpreted applying an Arrhenius relationship. The average Arrhenius constants found for sewer sediment particles and wastewater particles differed significantly from the average Arrhenius constant found for wastewater. The effects of dissolved oxygen concentration were interpreted applying a saturation type relationship. No differences between sewer sediment particles, wastewater particles and wastewater were found for the oxygen saturation coefficients.