Ji-Suk Cho
Inha University
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Publication
Featured researches published by Ji-Suk Cho.
Biotechnology and Bioprocess Engineering | 2007
Ji-Suk Cho; Hye Won Lee; Song-Jae Lee; Dong-Il Kim
The new technology, two-dimensional difference gel electrophoresis (2D DIGE), uses fluorescent dyes to simplify the process of detecting and matching proteins between multiple gels by allowing for the separation of up to three separate protein samples within the same gel. In this study, recombinant human cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (hCTLA4lg) was produced in transgenic rice suspension cell cultures and the intracellular proteins were analyzed by 2D DIGE. The highest level of hCTLA4Ig (25.4 mg/L) was obtained five days after induction. The intracellular proteins expressed at both the growth and induction culture stages were separated and analyzed using DeCyder software. At least 2,218 spots were detected with two-fold thresholds and 95% confidence. We found that 29 spots increased and 20 spots decreased in their intensities during the production of recombinant hCTLA4Ig. In addition, the 2D Western blot of hCTLA4Ig revealed that this fusion protein was expressed in a variety of isoforms.
Biotechnology and Bioprocess Engineering | 2018
Seung-Hoon Kang; Hong-Yeol Choi; Ji-Suk Cho; Su-Hwan Cheon; Ji-Yeon Kim; Brian B. Kim; Dong-Il Kim
A reproducible method for cryopreservation of transgenic rice cells (Oryza sativa L. cv. Dongjin) producing recombinant human cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (hCTLA4Ig) has been established. Here, we assessed recovery media and investigated recombinant protein homogeneity after long-term preservation. For recovery of cryopreserved transgenic rice cells, AA medium was suitable in terms of both morphology and production of hCTLA4Ig. There were no differences in cell growth, sugar consumption, and hCTLA4Ig production between non-cryopreserved and cryopreserved cells for up to 1 month. hCTLA4Ig produced from cryopreserved cells was identical that of hCTLA4Ig from non-cryopreserved cells, as determined by analysis of its molecular weight and isoforms. For long-term preservation, cell viability was stably maintained at 61% for 26 months. In conclusion, these results demonstrate the possibility for reproducible cryocell-banking of transgenic rice cells without changes in the characteristics of cells and target proteins.
Applied Microbiology and Biotechnology | 2007
Ji-Suk Cho; Seok-Mi Hong; Sung-Yeon Joo; Ji-Seon Yoo; Dong-Il Kim
KSBB Journal | 2002
Ji-Yeon Kim; Ji-Suk Cho; Ji-Suk Moon; Ik-Hwan Kim; Dong-Il Kim
한국생물공학회 학술대회 | 2004
Sung-Hun Choi; Song-Jae Lee; Ji-Suk Cho; Kyoung-Hoon Lee; Su-Hwan Cheon; Seok-Mi Hong; Hye-Jin Hong; Dong-Il Kim
한국생물공학회 학술대회 | 2004
Seok Mi Hong; Ji-Suk Cho; Song-Jae Lee; Hye-Jin Hong; Sung-Hun Choi; Dong-Il Kim
한국생물공학회 학술대회 | 2007
Ji-Seon Yoo; Su-Hwan Cheon; Ji-Suk Cho; Kyoung-Hoon Lee; Jun-Young Kwon; Dong-Il Kim
한국생물공학회 학술대회 | 2007
Ji-Seon Yoo; Ji-Suk Cho; Jung-Ae Lim; Dong-Il Kim
한국생물공학회 학술대회 | 2005
Ji-Suk Cho; Sung-Yeon Joo; Seok-Mi Hong; Song-Jae Lee; Dong-Il Kim
한국생물공학회 학술대회 | 2005
Sung-Yeon Joo; Kyoung-Hoon Lee; Ji-Suk Cho; Dong-Il Kim