Kyoung-Hoon Lee
Inha University
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Publication
Featured researches published by Kyoung-Hoon Lee.
Enzyme and Microbial Technology | 2012
Jun-Young Kwon; Kyoung-Hoon Lee; Su-Hwan Cheon; Dong-Il Kim
To enhance the production of hCTLA4Ig in transgenic rice suspension cell cultures, anoxic conditions were applied during the production phase. Under the anoxic conditions in sugar-depleted media, cell viability was reduced rapidly and protease activity increased compared to aerobic conditions. However, the maximum production level of hCTLA4Ig with sugar-depleted anoxic conditions was the same as that in aerobic conditions. In addition, the production of hCTLA4Ig under anoxic conditions reached a peak 2 days earlier than that in aerobic conditions. Addition of 30 mM glucose at the production phase under anoxic conditions markedly improved cell viability. A viability level over 65% could be maintained for more than 30 days. Repression of the RAmy3D promoter by residual sugar in the production of hCTLA4Ig was not observed under anoxic conditions with 30 mM glucose. In addition, the production periods of hCTLA4Ig was extended up to 30 days and the maximum production level of hCTLA4Ig under anoxic conditions was 2.1-fold higher. Therefore, anoxic conditions could be used for the enhanced production of hCTLA4Ig in transgenic rice cell cultures.
Journal of Microbiology and Biotechnology | 2009
Su-Hwan Cheon; Kyoung-Hoon Lee; Jun-Young Kwon; Sung-Hun Choi; Mi-Na Song; Dong-II Kim
Plant Cell Reports | 2012
Jun-Young Kwon; Kyoung-Hoon Lee; Su-Hwan Cheon; Hyun-Nam Ryu; Sun Jin Kim; Dong-Il Kim
Enzyme and Microbial Technology | 2006
Sung-Yeon Joo; Kyoung-Hoon Lee; Yong-Il Lee; Dong-Il Kim
한국생물공학회 학술대회 | 2004
Sung-Hun Choi; Song-Jae Lee; Ji-Suk Cho; Kyoung-Hoon Lee; Su-Hwan Cheon; Seok-Mi Hong; Hye-Jin Hong; Dong-Il Kim
Journal of Microbiology and Biotechnology | 2007
Su-Hwan Cheon; Kyoung-Hoon Lee; Jun-Young Kwon; Hyun-Nam Ryu; Dahyun Yu; Yong-Soo Choi; Dong-Il Kim
한국생물공학회 학술대회 | 2007
Mi-Na Song; Kyoung-Hoon Lee; Jun-Young Kwon; Jung-Ae Lim; Boreum Yun; Dong-Il Kim
KSBB Journal | 2006
Kyoung-Hoon Lee; Sang-Min Lim; Dong-Il Kim
Journal of Biotechnology | 2008
Jun-Young Kwon; Su-Hwan Cheon; Kyoung-Hoon Lee; Sung-Hun Choi; Dong-Il Kim
한국생물공학회 학술대회 | 2007
Ji-Seon Yoo; Su-Hwan Cheon; Ji-Suk Cho; Kyoung-Hoon Lee; Jun-Young Kwon; Dong-Il Kim