Jin-Hyuk Lim
Inha University
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Publication
Featured researches published by Jin-Hyuk Lim.
Enzyme and Microbial Technology | 2017
Hyun-Myoung Cha; Jin-Hyuk Lim; Jung-Heum Yeon; Jeong-Min Hwang; Dong-Il Kim
Terminal sialic acids on N-glycan of recombinant human erythropoietin are very important for in vivo half-life, as this glycoprotein has three N-glycosylation sites. N-acetylglucosaminyltransferases I, II, IV, and V (i.e. Mgat1, Mgat2, Mgat4, and Mgat5) catalyze the formation of a glycan antennary structure. These enzymes display different reaction kinetics for a common substrate and generally show low expression in Chinese hamster ovary (CHO) cells. Therefore, genetic control of Mgat expression is an effective method to increase sialic acid contents by enhancing glycan antennarity. To produce highly sialylated albumin-erythropoietin (Alb-EPO), we co-overexpressed the Mgat1 and Mgat4 genes in CHO cells and determined the optimal ratio of Mgat1:Mgat4 gene expression. All transfected cell lines showed increased gene expression of Mgat4, including Mgat1 overexpressing cell line. Sialic acid content of Alb-EPO was highest in co-transfected cells with excess Mgat4 gene, and these cells showed a higher tri- and tetra-antennary structure than control cells. Based on these results, we suggest that co-transfection of the Mgat1 and Mgat4 genes at a ratio of 2:8 is optimal for extension of antennary structures. Also, regulation of Mgat gene expression in the glycan biosynthesis pathway can be a novel approach to increase the terminal sialic acids of N-glycans.
한국생물공학회 학술대회 | 2017
Jin-Hyuk Lim; Hyun-Myoung Cha; Ji-Hee Lee; Na-Hee Kim; Ji-Hun Lee; Dong-Il Kim
한국생물공학회 학술대회 | 2017
Na-Hee Kim; Hyun-Myoung Cha; Jin-Hyuk Lim; Ji-Hee Lee; Ji-Hun Lee; Dong-Il Kim
한국생물공학회 학술대회 | 2017
Ji-Hee Lee; Hyun-Myoung Cha; Jin-Hyuk Lim; Ji-Hun Lee; Na-Hee Kim; Dong-Il Kim
한국생물공학회 학술대회 | 2017
Ji-Hun Lee; Hyun-Myoung Cha; Jin-Hyuk Lim; Ji-Hee Lee; Na-Hee Kim; Dong-Il Kim
Process Biochemistry | 2017
Hyun-Myoung Cha; Jin-Hyuk Lim; Kyung-Sun Lee; Dong-Il Kim
KSBB Journal | 2017
Jin-Hyuk Lim; Hyun-Myoung Cha; Heajin Park; Ha Hyung Kim; Dong-Il Kim
KSBB Journal | 2017
Kyung-Sun Lee; Hyun-Myoung Cha; Jin-Hyuk Lim; Dong-Il Kim
한국생물공학회 학술대회 | 2016
Ji-Hee Lee; Hyun-Myoung Cha; Jin-Hyuk Lim; Dong-Il Kim
한국생물공학회 학술대회 | 2015
Hyun-Myoung Cha; Joon-Serk Seo; Jin-Hyuk Lim; Soo-Ah Shin; Yeon-Kyeong Shin; Hye-Eun Shim; Dong-Il Kim