Jin-Seok Lee

Comparison of Growth Characteristics and Yield of Silage Corn Hybrids by Different Planting Dates at Paddy and Upland Field

ABSTRACTThis study was conducted to evaluate growth characteristics and yield of silage corn hybrids by different planting dates at paddy and upland field. Days to silking by different planting dates at paddy field were 81 days at planting on April 22, 70 days on May 20 and 62 days on June 10, respectively, and they were getting shorter as planting date delayed. There was no significant difference in days to silking between paddy and upland field. Plant height in paddy field was no significant difference between at planting on April 22 and on May 20, and it was shortened sharply at planting on June 10. There was no significant difference in plant height between paddy and upland field. There was no significant difference in lodging by different planting dates in paddy field. There was significant difference in lodging between paddy and upland field(P<0.05). There was no significant difference in stay-green by different planting dates in paddy field, and no significant difference between paddy and upland field. Ear ratios to total dry matter in paddy field were 43% at planting on April 22, 41% on May 20 and 28% on June 10, respectively, and it was lower as planting date delayed. There was no significant difference in ear ratios to total dry matter between paddy and upland field. Fresh yields in paddy field were lower with 14% at planting on May 20 and 32% on June 10 compared to on April 22 as planting date delayed. There was no significant difference in fresh yield between paddy and upland field. Dry matteryields in paddy field were higher with 9% at planting on May 20 and lower with 24% on June 10 compared to at planting on April 22 as planting date delayed. Dry matter yield in paddy field was lower with 9% compared to 1,931kg/10a at planting on April 22. TDN(Total digestible nutrients) yields in paddy field were higher with 5% at planting on May 20 and lower with 28% on June 10 compared to at planting on April 22 as planting date delayed. TDN yield in paddy field was lower with 11% compared to 1,340kg/10a at planting on April 22. (Key words:Paddy field, Silage corn, Planting date, Yield, Dry matter, TDN)

Yield of maize ( Zea mays L.) logistically declined with increasing length of the consecutive visible wilting days during flowering

Under future climate conditions, the frequency and severity of drought are expected to increase. Maize (Zea mays L.) is susceptible to drought, especially at flowering. The objective of this study was to investigate the effect of consecutive days of visible wilting (DAW) during tassel emergence using two types of Korean maize hybrids, Gwangpyeongok (Gwp; normal) and Ilmichal (Ilmi; waxy). Traits related to plant growth and yield were measured at harvest. Drought stress during tassel emergence significantly reduced the number of green leaves per plant. Silking was delayed due to drought stress during the stage of tassel emergence, although anthesis was only slightly affected, which resulted in an increase in the anthesis-silking interval (ASI). For each DAW, ASI increased by 1.6 days and 0.8 days for Gwp and Ilmi, respectively. The number of green leaves and ASI showed strong correlation with DAW length and grain yield, which suggested that those factors may be selective traits for drought tolerance at flowering. The filled grain number per plant declined logistically with increasing DAW due to reductions in kernels per row and percentage of filled grains. The filled grain number per plant significantly declined starting at 5 and 10 DAW in Gwp and Ilmi, respectively, which indicated that drought stress effects are dependent on drought severity and the genetic characteristics of specific hybrids. Probit analysis revealed that DAW causing 50% reduction in maize grain yield was about 4.7 and 6.3 days in Gwp and Ilmi, respectively, which resulted in about 13.6% and 9.2% reduction in grain yield per day of DAW, respectively. These results indicate that Gwangpyeongok is more sensitive to drought stress during flowering than Ilmichal.

Physiological and Protein Profiling Response to Drought Stress in KS141, a Korean Maize Inbred Line

Understanding the complex response mechanism of a crop to drought is the major step in the developing of tolerant genotypes. In our study, to investigate physiological traits and proteome dynamics, an inbred maize (Zea mays L.) line (KS141) was subjected to 10 days of water-withholding at the V5 or V6 leaf stage. The subsequent analysis of their physiological parameters revealed a decreased relative leaf water content, Fv/Fm, stomatal conductance, net CO2 assimilation rate, leaf transpiration, and water use efficiency, resulting in severe growth retardation of leaf area, stem length and width, aerial part, and root dry matter at 3 and 10 days after withholding water. However, aerial part and root dry matter were little changed during drought stress for 3 days. To understand the proteome dynamics during the 10-day drought stress in maize leaves, comparative proteome analysis was carried out between the well-watered and drought-treated leaves. Proteins were extracted using phenol extraction method from leaves with/without drought stress, and then separated by 2-DE. After 2-DE gel analyses, 14 differentially expressed protein spots were identified by MALDITOF mass spectrometry. Out of 14, eleven and three protein spots were found to be up- or down-regulated, respectively. Interestingly, stress-related proteins such as glutathione S-transferase, abscisic stress-ripening proteins, and pathogenesis-related proteins were increased by drought stress. Our study may provide molecular mechanisms and selective markers for drought tolerant maize genotypes.

Change of Seed Yield, Germination Rate and Hard Seed Rate with Harvest Time in Hairy Vetch (Vicia villosa Roth)

This experiment was conducted to investigate an optimum harvest time for hairy vetch (HV) seed production. The seeds were harvested at 5 times, 35, 42, 48, 54, and 58 days after flowering (DAF) in 2009 and 2010. In 2011, 3 times of harvest, 39, 49, and 53 DAF were done at Suwon, middle part of Korea. HV plants were harvested and threshed by manually at each time. Seed yield, yield components, germination rate, hard seed rate, and viviparous germination rate were investigated. The highest seed yield represented 54 DAF in 2009, 42 DAF in 2010, and 49 DAF in 2011. When the triticale, HV support plant, comes to early lodging, the time of highest yield was delayed. 1,000-seed weight linearly increased from 35 DAF to 54 DAF. The later harvested seed showed a trend of increasing germination and hard seed ratio. However, viviparous germination had occurred because of in rainy season. In conclusion, it was presumed that an optimum harvest time for safe seed production in HV should be during 42 DAF ~ 54 DAF before the rainy season considering seed yield, 1,000-seed weight and germination.

Profiling of differentially expressed proteins in mature kernels of Korean waxy corn cultivars using proteomic analysis

Maize (Zea mays L.) is the most important crop such as food for humans and feed for animals. Although new varieties of maize have been extensively developed in Korea, little is known about differences in the proteomes of mature kernels among maize varieties. Three Korean waxy corn cultivars (Ilmichal, Eolrukchal 1, and Heukjinjuchal) have previously been developed. The total protein content is higher in Ilmichal than in Eolrukchal 1 or Heukjinjuchal, while the starch and fatty acid contents are similar among these three Korean waxy corns. To uncover the differences in proteomic profiles among these three Korean waxy corns, we performed proteomic analysis and compared their protein compositions. We detected 37 differentially expressed protein spots and identified the proteins using MALDI-TOF mass spectrometry. Of these proteins, 37.8xa0% were identified as storage proteins, 18.9xa0% as stress-related proteins, and 18.9xa0% as metabolism-related proteins. Storage proteins (globulin-2) and stress-related proteins (heat shock proteins and general stress proteins) were highly expressed in Ilmichal or Heukjinjuchal. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of corresponding genes of five randomly selected proteins, including glyoxalase family protein (accession number, B6SGF3), globulin-2 (Q7M1Z8), heat shock protein 1 and 3 (B6TGQ2 and B6TDB5), and vicilin-like embryo storage protein (Q03865), showed that the expression levels of the tested genes were well-correlated with protein abundance, suggesting that these proteins are also differentially regulated at the transcriptional level. Taken together, these results provide a better understanding of proteomic differences among Korean waxy corn cultivars and may support further molecular breeding efforts.

Impact of the consecutive days of visible wilting on growth and yield during tassel initiation in maize ( Zea Mays L.)

Maize (Zea mays L.) is a major crop with different uses but shows significant susceptibility to drought stress. Recent climate change has caused prolonged drought stress, but in most countries maize production relies on rain-fed cultivation. This study was to investigate the impact of the consecutive days of visible wilting (DAW) during tassel initiation (V6) using two types of Korean maize hybrids, Gwangpyeongok (GWP) and Ilmichal (Ilmi). The traits related to plant growth and yield were measured at harvest. Drought stress during tassel initiation linearly reduced plant elongation and dry matter accumulation with the length of DAW increasing but did not affect the leaf number per plant. Our results from the simple linear regression analysis indicated that silking of GWP and Ilmi would be delayed by 0.78 and 0.61 days per DAW, respectively, which was similar to anthesis. Still, little change was observed for interval between anthesis and silking under the same condition. The kernels per row were very dependent on the length of DAW but the rows per ear were not. The percentage of filled grains and the 100- grain weight were significantly reduced only at 15- or 20-DAW and more markedly so in Gwangpyeongok at 20-DAW. The filled grain number per plant showed a gradual logistical decline mainly due to decreases in the kernels per row. All these reductions may be because initiation and growth of tassel, ear, and kernels and internode elongation beginning around V6 are sensitive to water deficits. Probit analysis revealed that the DAW to cause 50% reduction in plant grain yield was about 12 and 20 days in GWP and Ilmi, resulting in about 4.5 and 2.7% reduction a day of DAW, respectively. These results indicate that Gwangpyeongok may be more sensitive to drought stress during tassel initiation than Ilmichal.

Response of Growth Characteristics and Yield of Silage Corns at Different Regions

This study was carried out to compare growth characteristics and yield of silage corn hybrids at four different regions (Hwaseong, Dangjin, Gochang and Pyeongchang). Two domestic corn hybrids, `Kwangpyeongok` and `Gangdaok`, and two imported corn hybrids, `P3394` and `32P75` were used in this study. Plant height and ear height ratio were not significantly different among corn hybrids. `Kwangpyeongok` was the strongest of all corn hybrids for lodging. The others except `Kwangpyeongok` were similar to lodging. All corn hybrids were excellent to stay-green. Incidence of southern corn leaf blight (Bipolaris maydis) was low at all corn hybrids. `32P75` was 10.1%, the most of all corn hybrids for incidence of BSDV (black streaked dwarf virus). `Kwangpyeongok` and `Gangdaok` were stronger than `32P75` for BSDV. Fresh yield of `Kwangpyeongok` and `Gangdaok` were the most of all corn hybrids as 74.85 and 76.68 ton/ha and `P3394` was the least of all corn hybrids as 64.97 ton/ha. Dry matter yield as 18.83~21.92ton/ha and TDN yield as 12.73~14.52 ton/ha were not significantly different among corn hybrids.