Jingyue Xu

Chemical sensors and biosensors for the detection of melamine

Melamine is an emerging contaminant in milk, infant formula and pet food. In order to increase the “false” apparent protein content in food products, melamine has been artificially and illegally used as non-protein nitrogen additive. This review focuses on chemical sensors and biosensors for detecting melamine residue. We present the principles, the mechanisms and the performances of the sensors including optical sensors, electrochemical sensors, aptamer-based sensors and immunosensors. We also propose the future perspectives in developing sensors for the detection of melamine.

Aptamer-based fluorescent screening assay for acetamiprid via inner filter effect of gold nanoparticles on the fluorescence of CdTe quantum dots.

AbstractThis paper reports a novel aptamer-based fluorescent detection method for small molecules represented by acetamiprid based on the specific binding of aptamers with acetamiprid, and the inner filter effect (IFE) of gold nanoparticles (AuNPs) on the fluorescence of CdTe quantum dots (CdTe QDs). When CdTe QDs were mixed with AuNPs, the fluorescence of CdTe QDs was significantly quenched via IFE. The IFE efficiency could be readily modulated by the absorption and the aggregation state of AuNPs. The presence of salt could easily induce the aggregation of AuNPs, resulting in the fluorescence recovery of the quenched QDs. Acetamiprid-binding aptamer (ABA) could adsorb on the negatively charged AuNPs through the coordination interaction to protect AuNPs from salt-induced aggregation, so the fluorescence of CdTe QDs would be quenched by the IFE of AuNPs. However, the specific binding of ABA with acetamiprid could release the ABA from the surfaces of AuNPs and decrease the salt tolerance of AuNPs, so the IFE-decreased fluorescence of CdTe QDs was regained with the presence of acetamiprid, and the fluorescence enhancement efficiency was driven by the concentration of acetamiprid. Based on this principle, the aptamer-based fluorescent method for acetamiprid has been established and optimized. The assay exhibited excellent selectivity towards acetamiprid over its analogues and other pesticides which may coexist with acetamiprid. Under the optimum experiment conditions, the established method could be applied for the determination of acetamiprid with a wide linear range from 0.05 to 1.0 μM, and a low detection limit of 7.29 nM (3σ). Furthermore, this IFE-based method has been successfully utilized to detect acetamiprid in six types of vegetables, and the results were in full agreement with those from HPLC and LC-MS. The proposed method displays remarkable advantages of high sensitivity, rapid analysis, excellent selectivity, and would be suitable for the practical application of target screening in real samples. Graphical Abstractᅟ

Aptamers-Based Sensing Strategy for 17β-Estradiol Through Fluorescence Resonance Energy Transfer Between Oppositely Charged CdTe Quantum Dots and Gold Nanoparticles

A novel aptamers-based fluorescence resonance energy transfer (FRET) assay was developed employing the oppositely charged thioglycolic acid-capped CdTe quantum dots (TGA-CdTe QDs) and cysteamine-stabilized gold nanoparticles (cysteamine-AuNPs). The fluorescence of TGA-CdTe QDs was significantly quenched with addition of cysteamine-AuNPs via the FRET. The FRET process can be modulated by 17β-estradiol in that the specific recognition between 17β-estradiol and aptamers could show different effects on the aptamers-mediated aggregation of cysteamine-AuNPs, and correspondingly adjust the FRET process. The feasibility of the method has been demonstrated by carrying out the detection of 17β-estradiol with the wide linear range from 0.5·ng mL-1 to 150 ng·mL-1 and the low detection limit of 0.057 ng·mL-1. The established assay exhibited favorable selectivity towards 17β-estradiol over other endocrine disrupting compounds and probably coexisting chemicals in real samples. Furthermore, the assay has been successfully applied to detect 17β-estradiol in real tap water samples and feeds samples with good performance. The results were in full consistence with those from HPLC method, indicating the reliability of the detection system. The aptamers-based FRET assay is expected to offer a new opportunity for the rapid analysis of 17β-estradiol in real samples.