Joan Ellen Brooks

Purification and characterization of C·BamHI, a regulator of the BamHI restriction-modification system

The bamHIC gene, controlling the BamHI restriction-modification (R-M) system can functionally be replaced by providing pvuIIC or smaIC in trans. C.BamHI, the protein product encoded by bamHIC, has been purified and shown to bind a 345-bp DNA fragment within the BamHI R-M system.

Characterization of clones of the BamHI methyltransferase gene

We are characterizing different subclones of the bamHIM gene. One clone carries a 2.2-kb HindIII fragment that contains the MTase gene and the N-terminal end of the endonuclease gene

Cloning and characterization of the BamHI restriction modification system

The BamHI system has been cloned into Escherichia coli in two steps. First the bamHIM gene was cloned by selecting for its expression, a second gene was also cloned whose product modifies the same sequence