Joanna Honke

Influence of Fermentation Conditions on Glucosinolates, Ascorbigen, and Ascorbic Acid Content in White Cabbage (Brassica oleracea var. capitata cv. Taler) Cultivated in Different Seasons

The content of glucosinolates (GLS), ascorbigen, and ascorbic acid in white cabbage (Brassica oleracea var. capitata cv. Taler) cultivated in different seasons (summer and winter) was determined, before and after spontaneous and starter-induced fermentation. Different salt concentrations (0.5% NaCl or 1.5% NaCl) were used for sauerkraut production. Glucoiberin, sinigrin, and glucobrassicin were dominating in raw white cabbage cultivated either in winter or summer seasons. Ascorbigen precursor, glucobrassicin, was found higher in cabbage cultivated in winter (2.54 micromol/g dw) than those grown in summer (1.83 micromol/g dw). Cabbage fermented for 7 d was found to contain only traces of some GLS irrespective of the fermentation conditions used. Ascorbigen synthesis occurred during white cabbage fermentation. Brining cabbage at low salt concentration (0.5% NaCl) improved ascorbigen content in sauerkraut after 7 d of fermentation at 25 degrees C. The highest ascorbigen concentration was observed in low-sodium (0.5% NaCl) sauerkraut produced from cabbage cultivated in winter submitted to either natural (109.0 micromol/100 g dw) or starter-induced fermentation (108.3 and 104.6 micromol/100 g dw in cabbages fermented by L. plantarum and L. mesenteroides, respectively). Ascorbic acid content was found higher in cabbage cultivated in summer and fermentation process led to significant reductions. Therefore, the selection of cabbages with high glucobrassicin content and the production of low-sodium sauerkrauts may provide enhanced health benefits towards prevention of chronic diseases.

Natural fermentation of lentils : Influence of time, concentration, and temperature on the kinetics of hydrolysis of inositol phosphates

Lentil (Lens culinaris var vulgaris) flour was naturally fermented for 96 h at various conditions of concentration (79, 150 and 221 g litre -1 ) and temperature (28°C, 35°C and 42°C). The content of total inositol phosphates (IP-total) and individual inositol phosphates (hexa- (IP 6 ), penta- (IP 5 ), tetra- (IP 4 ) and tri- (IP 3 ) phosphates) were analysed to establish the changes of these compounds during natural fermentation of lentils. The preparation of the lentil suspension brought about 16-27% reduction of the total inositol phosphates. At the end of 96 h of natural fermentation maximum IP loss (70-75%) was achieved for an experiment carried out at minimum concentration. For IP 6 , the largest decrease was achieved at the highest temperature, the fermentation condition that also brought about the highest IP 5 content.

Effect of Light Conditions on the Contents of Glucosinolates in Germinating Seeds of White Mustard, Red Radish, White Radish, and Rapeseed

The study was aimed at determining the effect of light conditions on contents of glucosinolates (GLS) in germinating seeds of white mustard, red radish, white radish, and rapeseed. The seeds were germinated in light and dark, at 25 degrees C, for up to 7 days. As compared to the nongerminated seeds, in seeds exposed to light and germinated for 4, 5, 6, and 7 days the content of total GLS was observed to decrease by 30 to 70% depending on the species. Germination in conducted the dark for the respective periods of time resulted in decreases of total GLS not exceeding 25%. The changes in the concentration of total GLS were attributed to aliphatic GLS predominating in seeds, yet in the case of white mustard to sinalbin belonging to aralkyl glucosinolates. Although seeds germinated in the dark, as compared to those exposed to light, were characterized by a higher total content of indole GLS, the percentage contribution of that group of compounds in white mustard, red radish, and white radish remained at a similar level, irrespective of germination time. Only in the case of rapeseed was the percentage of the sum of indole GLS observed to increase from 17 to up to 45% once the seeds were exposed to light and to 50% once they were germinated in the dark.

Diet shapes the ability of human intestinal microbiota to degrade phytate--in vitro studies.

Investigation of intestinal bacterial groups involved in phytate degradation and the impact of diets with different phytate contents on phytase activity.

Effect of frying various legumes under optimum conditions on amino acids, in vitro protein digestibility, phytate and oligosaccharides.

The effects of optimum conditions of frying soybean, broad bean and Polish pea on proximate analysis and amino acid composition were investigated. Maximum water absorption of 192.93, 129.05 and 148.68% for soybean, broad bean and Polish pea was reached after soaking periods of 16.41, 17.84 and 16.45 h respectively. Sensory evaluation of different temperatures (150, 170 and 190°C) and frying times (5, 10 and 15 min) for preparing soybean, broad bean and Polish pea indicated that there was a significant difference (P < 0.01) between treatments. The most acceptable fried soybean, broad bean or Polish pea was obtained after frying at 190°C for 5 min. All the processed legumes under investigation had higher protein digestibility as measured in vitro and minimum reduction in total amino acid composition. The optimum frying process is an effective method for lowering phytate content (myo-inositol hexaphosphate) by 89.77, 67.70 and 82.88% respectively as well as causing considerable loss of total oligosaccharides by 64.35, 16.16 and 49.44%.

Exposure of breastfed infants to quercetin after consumption of a single meal rich in quercetin by their mothers

SCOPE The exposure to quercetin (Q) has not been studied in breastfed infants whose mothers were consuming a Q-rich diet. The objective of the study was to determine whether plant-origin antioxidant-Q passes from the mothers diet to her milk and to calculate the pharmacokinetic parameters of this phenomenon. METHODS AND RESULTS Eleven breastfeeding women were included in this controlled case study. Volunteers followed a Q-restricted diet for 5 consecutive days with the exception of the 3rd day when they received a single meal providing 1 mg of Q per kg of body weight. Urine analysis showed the presence of Q already in the first collected samples after the test (1.5-4 h), which indicated its rapid absorption from the meal. The Cmax = 68 ± 8.44 nmol/L concentration of Q in the milk was calculated for Tmax = 11.89 ± 3.37 h. It was significantly different (p = 0.007) from 40 nmol/L and (p = 0.016) from 42 nmol/L of Q concentration before and 48 h after the test, respectively. CONCLUSIONS Q was shown to be a component of human milk at the nmol/L level. Infants breastfed by mothers consuming a diet rich in Q are exposed to a dose of approximately 0.01 mg of Q daily.

Evaluation of Seasonal Variations in the Glucosinolate Content in Leaves and Roots of Four European Horseradish (Armoracia rusticana) Landraces

Abstract In comparison with other cruciferous vegetables, horseradish has rarely been the object of scientific research, and the knowledge about the composition, content and distribution of glucosinolates (GLS) in different organs of horseradish plants is limited. Therefore, the aim of this study was to evaluate changes in the GLS content in leaves and roots of four horseradish landraces during the growing season. The presence of 13 GLS was determined in the examined horseradish tissues, and glucoraphanin, glucoraphenin and napoleiferin were noted for the first time in the species. During the growing season, the content of individual GLS changed significantly. The rate and direction of these changes varied across the examined landraces and plant organs. In the leaves, between May and June, the content of sinigrin, the main GLS in all horseradish landraces, decreased in Bavarian (40%) and Hungarian (11%) horseradish, increased (22%) in Creamy horseradish, whereas in Danish horseradish, the difference was not significant. Despite the changes observed in the first two months, the highest content of sinigrin was noted in July in all horseradish landraces. During the growing season (August-October), the content of sinigrin fluctuated in the roots of Creamy and Danish landraces, reaching the highest level in October and September, respectively, whereas in the roots of Hungarian and Bavarian landraces, sinigrin concentrations continued to increase and peaked in October. Changes in the content of other, minor GLS during the growing season often differed from those noted in sinigrin levels.