Joanna Płonka

Food analysis – samples preparation and chromatographic methods in determination of selected biogenic amines, methylxanthines and water-soluble vitamins

The expanding demands of consumers requires food quality control to be improved all the time. Higher concentration of biogenic amines can lead to food toxicity, for example they can cause migraine headaches in people who consume products rich of serotonin or tyramine. Methylxanthines (caffeine and metabolites) can also have side effects to human comfort – high doses can lead to unrest, irritation or insomnia. Most vitamins are supplied to humans only as a part of food meals or dietary supplements. Knowledge about their concentration in food can be useful for composing various diets. In this work specification of complete sample preparation parameters for extraction of the compounds from food matrices has been reviewed. Particular attention was given to the preparation stage as well as to extraction methods that have been used. The second part of the work presents data from chromatographic methods for determination and separation of selected biogenic amines, methylxanthines and water-soluble vitamins in food. Stationary and mobile phases, detection methods as well as validation data have been reviewed. This publication is a comprehensive compendium of analytical procedures for food analyses of the previously mentioned compounds.

Determination of Biogenic Amines and Vitamins in Urine Samples with HPLC

Abstract An HPLC system for simultaneous separation and marking of biogenic amines and their metabolites from catecholamines group: dopamine (DA), epinephrine (E), normetanephrine (NMN), metanephrine (MN), 3,4-dihydroxyphenylacetic acid (DOMA), 3-metoxy-4-hydroxyphenyl-glycol (MHPG), homovanillic acid (HVA) and indoloamines group: serotonin (5HT) and 5-hydroxyindole-3-acetic acid (5HIAA), as well as water soluble vitamins B-1, B-2, B-3, B-6, B-12, and C has been developed. The RP18e column with RP18 precolumn and DAD serial connected with a fluorescence detector have been used. Gradient elution with acetate buffer (pH = 4.66) with methanol has been applied. The developed system has been used for marking the examined compounds in urine samples.

Methods of biological fluids sample preparation – biogenic amines, methylxanthines, water‐soluble vitamins

In recent years demands on the amount of information that can be obtained from the analysis of a single sample have increased. For time and economic reasons it is necessary to examine at the same time larger number of compounds, and compounds from different groups. This can best be seen in such areas as clinical analysis. In many diseases, the best results for patients are obtained when treatment fits the individual characteristics of the patient. Dosage monitoring is important at the beginning of therapy and in the full process of treatment. In the treatment of many diseases biogenic amines (dopamine, serotonin) and methylxanthines (theophylline, theobromine, caffeine) play an important role. They are used as drugs separately or in combination with others to support and strengthen the action of other drugs - for example, the combination of caffeine and paracetamol. Vitamin supplementation may be also an integral part of the treatment process. Specification of complete sample preparation parameters for extraction of the above compounds from biological matrices has been reviewed. Particular attention was given to the preparation stage and extraction methods. This review provides universal guidance on establishing a common procedures across laboratories to facilitate the preparation and analysis of all discussed compounds.

Monitoring of biogenic amines and drugs of various therapeutic groups in urine samples with use of HPLC.

A high-performance liquid chromatography method for simultaneous separation and determination of biogenic amines [dopamine, epinephrine, serotonin and its six metabolites (normetanephrine, metanephrine, 3,4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylglycol, homovanilic acid and 5-hydroxyindoloacetic acid)] with drugs from different therapeutically groups [analgesics (paracetamol, metamizol), diuretics (furosemide) and antibiotics (cefazolin, fluconazole)] was developed. A chromatographic column with pre-column with octadecylsilane phase (C18e ) and two detectors - diode array serial connected and fluorescence - was used. Gradient elution of mixture of acetate buffer (pH 4.66) and methanol as a mobile phase was applied. The limit of detection (LOD) of 8-10 ng/mL and limit of quantitation (LOQ) of 24-30 ng/mL for biogenic amines, as well as the LOD of 50-100 ng/mL and the LOQ of 150-300 ng/mL for drugs, were determined. The applied sample preparation method allowed recoveries of 93% for the biogenic amines and 92% for the drugs to be achieved. The developed procedure has been applied to simultaneous determination of the examined compounds in urine samples and could be used in clinical analysis.

Quality Control of Plant-Based Foods in Terms of Nutritional Values: Influence of Pesticides Residue and Endogenous Compounds

Abstract Agrochemicals not only increase the efficiency of crop but also accumulate in plant tissues. Moreover, according to the latest reports, pesticides disrupt the natural biochemical processes in plant tissue. In the context of food quality, control monitoring of the metabolism of pesticides in plant organisms becomes particularly essential. Therefore special attention should be paid to the determination not only of pesticides residue but also endogenous compounds (e.g., vitamins, biogenic amines), since their biosynthesis may be interfered by agrochemicals. In this chapter, the quality control problems related to pesticides residue and selected endogenous compounds in food of plant origin will be discussed. Particular emphasis will be placed on the interactions between the previously mentioned compounds leading to changes of nutrient parameters of food. Analytical problems with the determination of agrochemicals, their metabolites, vitamins, and catecholamines and indoloamin will be discussed. The work will be a compendium of information on the interactions between pesticides and certain endogenous compounds in plants. Concurrently it will include a critical discussion of analytical issues related to food control on the contents of the previously mentioned compounds.