John D. Pantis

Long-term observation of a pollination network: fluctuation in species and interactions, relative invariance of network structure and implications for estimates of specialization.

We analysed the dynamics of a plant-pollinator interaction network of a scrub community surveyed over four consecutive years. Species composition within the annual networks showed high temporal variation. Temporal dynamics were also evident in the topology of the network, as interactions among plants and pollinators did not remain constant through time. This change involved both the number and the identity of interacting partners. Strikingly, few species and interactions were consistently present in all four annual plant-pollinator networks (53% of the plant species, 21% of the pollinator species and 4.9% of the interactions). The high turnover in species-to-species interactions was mainly the effect of species turnover (c. 70% in pairwise comparisons among years), and less the effect of species flexibility to interact with new partners (c. 30%). We conclude that specialization in plant-pollinator interactions might be highly overestimated when measured over short periods of time. This is because many plant or pollinator species appear as specialists in 1 year, but tend to be generalists or to interact with different partner species when observed in other years. The high temporal plasticity in species composition and interaction identity coupled with the low variation in network structure properties (e.g. degree centralization, connectance, nestedness, average distance and network diameter) imply (i) that tight and specialized coevolution might not be as important as previously suggested and (ii) that plant-pollinator interaction networks might be less prone to detrimental effects of disturbance than previously thought. We suggest that this may be due to the opportunistic nature of plant and animal species regarding the available partner resources they depend upon at any particular time.

Evaluating the Connectivity of a Protected Areas' Network under the Prism of Global Change: The Efficiency of the European Natura 2000 Network for Four Birds of Prey

Climate and land use changes are major threats to biodiversity. To preserve biodiversity, networks of protected areas have been established worldwide, like the Natura 2000 network across the European Union (EU). Currently, this reserve network consists of more than 26000 sites covering more than 17% of EU terrestrial territory. Its efficiency to mitigate the detrimental effects of land use and climate change remains an open research question. Here, we examined the potential current and future geographical ranges of four birds of prey under scenarios of both land use and climate changes. By using graph theory, we examined how the current Natura 2000 network will perform in regard to the conservation of these species. This approach determines the importance of a site in regard to the total network and its connectivity. We found that sites becoming unsuitable due to climate change are not a random sample of the network, but are less connected and contribute less to the overall connectivity than the average site and thus their loss does not disrupt the full network. Hence, the connectivity of the remaining network changed only slightly from present day conditions. Our findings highlight the need to establish species-specific management plans with flexible conservation strategies ensuring protection under potential future range expansions. Aquila pomarina is predicted to disappear from the southern part of its range and to become restricted to northeastern Europe. Gyps fulvus, Aquila chrysaetos, and Neophron percnopterus are predicted to locally lose some suitable sites; hence, some isolated small populations may become extinct. However, their geographical range and metapopulation structure will remain relatively unaffected throughout Europe. These species would benefit more from an improved habitat quality and management of the existing network of protected areas than from increased connectivity or assisted migration.

Spatial heterogeneity of ambient sound at the habitat type level: ecological implications and applications

While spatial heterogeneity is one the most studied ecological concepts, few or no studies have dealt with the subject of ambient sound heterogeneity from an ecological perspective. Similarly to ambient light conditions, which have been shown to play a significant role in ecological speciation, we investigated the existence of ambient sound heterogeneity and its possible relation to habitat structure and specifically to habitat types (as syntaxonomically defined ecological units). Considering that the structure and composition of animal communities are habitat type specific and that acoustic signals produced by animals may be shaped by the habitat’s vegetation structure, natural soundscapes are likely to be habitat specific. We recorded ambient sound in four forest and two grassland habitat types in Northern Greece. Using digital signal techniques and machine learning algorithms (self organizing maps, random forests), we concluded that ambient sound is not only spatially heterogeneous, but is also directly related to habitat type structure, pointing towards the existence of habitat type specific acoustic signatures. We provide evidence of the importance of soundscape heterogeneity and ambient sound signatures and a possible solution to the social cues versus vegetation characteristics debate in habitat selection theory.

Spatiotemporal analysis of an acoustic environment: interactions between landscape features and sounds

So far landscape analysis meant analysis of the spatial pattern of land cover or land use. However, biological organisms do not perceive the landscape only as land cover or land use, but they use all their senses, in order to become familiar with and react to their surroundings. We analyzed the acoustic environment as an additional layer of spatial information in landscape analysis, shortening the monopoly of visual patterns as landscape descriptors. We recorded sounds from a rural protected area into seven categories based on their origin, and examined their spatiotemporal variability and their correlation with landscape characteristics. The sounds were distinguished as Foreground or Background sounds. Foreground sounds correspond to sharp sounds originating near the observer and usually are understood as signals of urgent information, triggering reactions; while background sounds carry information over longer distances and may be used as landmarks to help individuals find their bearing even in the absence of visual signs. We found that the acoustic environment varies both temporally and spatially reflecting anthropogenic, geophysical and biological activities. The spatial pattern of the background sounds correlates, to an extent, with the visually perceived landscape features, but it does not correlate with the spatial pattern of the foreground sounds, which do not correlate strongly with the landscape pattern. This spatial pattern mismatch between acoustic environment and landscape, along with the highly dynamic nature of the acoustic environment compared to the relatively static nature of the land cover and land use spatial pattern highlight a limitation of the classical landscape analysis, and expands our understanding of the cognitive landscape.

Spatio-temporal variability in human and natural sounds in a rural landscape

The study of landscape structure and functions, including the underlying ecological and anthropogenic processes has traditionally relied on visual aspects without considering information of non-visionary cues, e.g. auditory. In this work we applied a complementary approach for the study of landscapes using qualitative information for the sonic environment. In particular we studied the qualitative linkages between landscape structure and functions and daily sound patterns. The main objectives were the investigation of the spatial and temporal variability in sound perception, and the identification of the dominant sound categories (anthropogenic, biological, geophysical originated sounds) in relation to landscape characteristics. Our results showed significant spatio-temporal variability in the intensity of different sound categories, which reflects distinct soundscape patterns. Temporal sound variability reflected the daily cycle of anthropogenic activities and biological processes, whereas the spatial sound viability was mainly shaped by landscape attributes. The combination of the visual landscape information and its emergent acoustic profile enhances our perception and understanding of nature and this integrated approach may have many practical applications in landscape management, monitoring and planning.

Knowledge and Attitudes Regarding Sea Turtles in Elementary Students on Zakynthos, Greece

Abstract The authors constructed a 32-item survey instrument to measure knowledge and aspects of attitudes (issue understanding and concern, locus of control, and verbal commitment) regarding sea turtle conservation on Zakynthos, Greece. It was completed by 332 5th and 6th grade students from 21 classes in 3 geographic settings. The results indicate low knowledge scores, but high score levels for attitudes. Knowledge, understanding and/or concern, and locus of control significantly correlated with grade level. Overall, a significant positive correlation between knowledge and attitudes was demonstrated. The findings of the study may be useful in designing environmental education programs to promote sea turtle conservation.

Comparison of chlorophyll fluorescence and some heavy metal concentrations in Sonchus spp. and Taraxacum spp. along an urban pollution gradient

Chlorophyll-α fluorescence induction kinetics of dark-adapted leaves of Sonchus spp. were measured in plants from areas of differing levels of urban pollution. Significant increases (∼ 200%) in the minimal (Fo) and maximal (Fm) fluorescence and decreases (∼ 50%) in the half-rise time from Fo to FM (t12) were observed for plants in areas of high pollution. A stress index factor (SIF) was derived based on the variation in these variables, using canonical variance analysis, which increased with increasing pollution levels. Significant linear relationhips between the fluorescence parameters of Sonchus spp. and Taraxacum spp. indicate that both plants exhibit the same changes in their chlorophyll fluorescence patterns in response to the pollution gradient. The ratio of Fm/Fo had a mean value of 5.2 ± 0.24 for Taraxacum spp. and 4.9 ± 0.23 Sonchus spp. No significant variation in the ratio of variable to maximal fluorescence (Fv/Fm) was observed (0.75–0.82) which indicated that the efficiency of the primary photochemistry of photosystem II was not directly affected by pollution level. The concentrations of Pb, Zn and Cu in the soil and plant tissue were higher in areas of higher traffic density and air pollution. Individual or combinations of the metal concentrations significantly accounted for at least 53% of the variation in SIF for both Taraxacum spp. and Sonchus spp.

Phenological response of sea turtles to environmental variation across a species' northern range

Variations in environmental parameters (e.g. temperature) that form part of global climate change have been associated with shifts in the timing of seasonal events for a broad range of organisms. Most studies evaluating such phenological shifts of individual taxa have focused on a limited number of locations, making it difficult to assess how such shifts vary regionally across a species range. Here, by using 1445 records of the date of first nesting for loggerhead sea turtles (Caretta caretta) at different breeding sites, on different continents and in different years across a broad latitudinal range (25–39° ′N), we demonstrate that the gradient of the relationship between temperature and the date of first breeding is steeper at higher latitudes, i.e. the phenological responses to temperature appear strongest at the poleward range limit. These findings support the hypothesis that biological changes in response to climate change will be most acute at the poleward range limits and are in accordance with the predictions of MacArthurs hypothesis that poleward range limit for species range is environmentally limited. Our findings imply that the poleward populations of loggerheads are more sensitive to climate variations and thus they might display the impacts of climate change sooner and more prominently.

Grazing effects on plant functional group diversity in Mediterranean shrublands

Grazing is one of the prevalent human activities that even today are taking place inside protected areas with direct or indirect effects on ecosystems. In this study we analyzed the effects of grazing on plant species diversity, plant functional group (PFG) diversity and community composition of shrublands. We analyzed plant diversity data from 582 sampling plots located in 66 protected areas of the Greek Natura 2000 network, containing in total 1102 plant species and subspecies. We also classified a priori all plant species in seven PFGs: annual forbs, annual grasses/sedges, legumes, perennial forbs, perennial grasses/sedges, small shrubs and tall shrubs. For each site, grazing intensity was estimated in four classes (no grazing, low, medium and high grazing intensity). We found that, at the spatial and temporal scale of this study, as grazing intensity increased, so did total species richness. However, each PFG displayed a different response to grazing. Short-lived species (annual grasses or forbs and legumes) benefited from grazing and their species richness and proportion in the community increased with grazing. Perennial grasses and forbs species richness increased with grazing intensity, but their dominance decreased, since their proportion in the community declined. Short shrub species richness remained unaffected by grazing, while tall shrub diversity decreased. Finally, in sites without grazing the spatial pattern of species richness of the different PFGs was not congruent with each other, while in grazed sites they were significantly positively correlated (with the exception of tall shrubs). This finding may imply that grazing is a selective pressure organizing the community structure, and imposing a certain contribution of each PFG. So, in Mediterranean shrublands in protected areas with a long historical record of grazing, it seems that grazing promotes species diversity and its continuation on a portion of the landscape may be a necessary part of an effective management plan.

Evidence-based management to regulate the impact of tourism at a key marine turtle rookery on Zakynthos Island, Greece

This study evaluates how key beach features influence suitability for nesting by Endangered loggerhead marine turtles Caretta caretta at an internationally important rookery on Zakynthos Island, Greece. During 2007–2009 we assimilated information on beach structure (elevation above sea level and width), the distribution of all nesting (turtle tracks that resulted in nests) and non-nesting (turtle tracks that did not result in nests) turtle emergences from the sea along 6 km of beach, nest placement parameters (distance from sea and elevation above sea level), and beach use by visitors. We found that turtles preferentially emerged on steeper sections of beach, with higher nesting densities occurring on the most environmentally stable beaches. Elevation was a more reliable indicator of nest placement (1 m above sea level) than distance to shore. However, because nests on steeper slopes are located closer to shore, the risk of damage by tourism is increased in such areas. We calculated a potential 36% overlap of natural nest locations with use of the beach by tourists; however, the recorded overlap was 7% because of existing management protocols. This overlap could be further reduced by focusing conservation effort (i.e. further restricting use by people) on beach sections with the steepest inclines. For example, slopes of > 22° comprise 1 km of total beach area annually, the closure of which (above the immediate shoreline to allow passage) would completely protect 50% of nests. This study shows the value of evidence-based management as a practical scientific tool to conserve threatened species in dynamic protected areas that are of both environmental and economic importance.