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Dive into the research topics where John J. Sharp is active.

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Featured researches published by John J. Sharp.


Nature Genetics | 1997

Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice

Elizabeth Simpson; Carol C. Linder; Evelyn E. Sargent; Muriel T. Davisson; Larry E. Mobraaten; John J. Sharp

Targeted mutagenesis in mice, a powerful tool for the analysis of gene function and human disease, makes extensive use of 129 mouse substrains. Although all are named 129, we document that outcrossing of these substrains, both deliberate and accidental, has lead to extensive genetic variability among substrains and embryonic stem cells derived from them. This clearer understanding of 129 substrain variability allows consideration of its negative impact on targeting technology, including: homologous recombination frequencies, preparation of inbred animals, and availability of appropriate controls. Based on these considerations we suggest a number of recommendations for future experimental design.


Nature Genetics | 2005

Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells

Robert S. Ohgami; Dean R. Campagna; Eric L. Greer; Brendan Antiochos; Alice McDonald; Jing Chen; John J. Sharp; Yuko Fujiwara; Jane E. Barker; Mark D. Fleming

The reduction of iron is an essential step in the transferrin (Tf) cycle, which is the dominant pathway for iron uptake by red blood cell precursors. A deficiency in iron acquisition by red blood cells leads to hypochromic, microcytic anemia. Using a positional cloning strategy, we identified a gene, six-transmembrane epithelial antigen of the prostate 3 (Steap3), responsible for the iron deficiency anemia in the mouse mutant nm1054. Steap3 is expressed highly in hematopoietic tissues, colocalizes with the Tf cycle endosome and facilitates Tf-bound iron uptake. Steap3 shares homology with F420H2:NADP+ oxidoreductases found in archaea and bacteria, as well as with the yeast FRE family of metalloreductases. Overexpression of Steap3 stimulates the reduction of iron, and mice lacking Steap3 are deficient in erythroid ferrireductase activity. Taken together, these findings indicate that Steap3 is an endosomal ferrireductase required for efficient Tf-dependent iron uptake in erythroid cells.


Journal of Cell Biology | 1997

Small, Membrane-bound, Alternatively Spliced Forms of Ankyrin 1 Associated with the Sarcoplasmic Reticulum of Mammalian Skeletal Muscle

Daixing Zhou; Connie S. Birkenmeier; McRae W. Williams; John J. Sharp; Jane E. Barker; Robert J. Bloch


Blood | 2005

nm1054: a spontaneous, recessive, hypochromic, microcytic anemia mutation in the mouse

Robert S. Ohgami; Dean R. Campagna; Brendan Antiochos; Emily B. Wood; John J. Sharp; Jane E. Barker; Mark D. Fleming


Physiology & Behavior | 2001

Transgenic and knockout databases: Behavioral profiles of mouse mutants

Anna V. Anagnostopoulos; Larry E. Mobraaten; John J. Sharp; Muriel T. Davisson


Genomics | 1998

An alternative first exon in the distal end of the erythroid ankyrin gene leads to production of a small isoform containing an NH2-terminal membrane anchor.

Connie S. Birkenmeier; John J. Sharp; Elaine J. Gifford; Susan A. Deveau; Jane E. Barker


Methods of Molecular Biology | 2001

Genetically Engineered Mice

John J. Sharp; Carol C. Linder; Larry E. Mobraaten


Behavioural Brain Research | 2001

Availability and characterization of transgenic and knockout mice with behavioral manifestations: where to look and what to search for.

Anna V. Anagnostopoulos; John J. Sharp; Larry E. Mobraaten; Janan T. Eppig; Muriel T. Davisson


Laboratory Animal Medicine (Second Edition) | 2002

Chapter 27 – Genetic Monitoring

John J. Sharp; Evelyn E. Sargent; Peter A. Schweitzer


Archive | 2013

mutation in the mouse nm1054: a spontaneous, recessive, hypochromic, microcytic anemia

Mark D. Fleming; Robert S. Ohgami; Dean R. Campagna; Brendan Antiochos; Emily B. Wood; John J. Sharp; E Jane

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Dean R. Campagna

University of Oklahoma Health Sciences Center

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Jane E. Barker

Icahn School of Medicine at Mount Sinai

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Mark D. Fleming

University of Oklahoma Health Sciences Center

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Muriel T. Davisson

Johns Hopkins University School of Medicine

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Carol C. Linder

Washington State University

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Connie S. Birkenmeier

Gulf Coast Regional Blood Center

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Alice McDonald

Millennium Pharmaceuticals

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