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Dive into the research topics where John W. Morley is active.

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Featured researches published by John W. Morley.


Vision Research | 2009

Focal activation of the feline retina via a suprachoroidal electrode array

Yan T. Wong; Spencer C. Chen; Jongmo Seo; John W. Morley; Nigel H. Lovell; Gregg J. Suaning

This paper presents the results of the first investigations into the use of bipolar electrical stimulation of the retina with a suprachoroidal vision prosthesis, and the effects of different electrode configurations on localization of responses on the primary visual cortex. Cats were implanted with electrodes in the suprachoroidal space, and electrically evoked potentials were recorded on the visual cortex. Responses were elicited to bipolar and monopolar stimuli, with each stimulating electrode coupled with either six-return electrodes, two-return electrodes, or a single-return electrode. The average charge threshold to elicit a response with bipolar stimulation and six-return electrodes was 76.47+/-8.76 nC. Bipolar stimulation using six-return electrodes evoked responses half the magnitude of those elicited with a single or two-return electrodes. Monopolar stimulation evoked a greater magnitude, and area of cortical activation than bipolar stimulation. This study showed that suprachoroidal, bipolar stimulation can elicit localized activity in the primary visual cortex, with the extent of localization and magnitude of response dependent on the electrode configuration.


Journal of Neurophysiology | 2009

Direct activation and temporal response properties of rabbit retinal ganglion cells following subretinal stimulation.

David Tsai; John W. Morley; Gregg J. Suaning; Nigel H. Lovell

In the last decade several groups have been developing vision prostheses to restore visual perception to the profoundly blind. Despite some promising results from human trials, further understanding of the neural mechanisms involved is crucial for improving the efficacy of these devices. One of the techniques involves placing stimulating electrodes in the subretinal space between the photoreceptor layer and the pigment epithelium to evoke neural responses in the degenerative retina. This study used cell-attached and whole cell current-clamp recordings to investigate the responses of rabbit retinal ganglion cells (RGCs) following subretinal stimulation with 25-mum-diameter electrodes. We found that direct RGC responses with short latency (</=2 ms using 0.1-ms pulses) could be reliably elicited. The thresholds for these responses were reported for on, off, and on-off RGCs over pulse widths 0.1-5.0 ms. During repetitive stimulation these direct activation responses were more readily elicited than responses arising from stimulation of the retinal network. The temporal spiking characteristics of RGCs were characterized as a function of stimulus configurations. We found that the response profiles could be generalized into four classes with distinctive properties. Our results suggest that for subretinal vision prostheses short pulses are preferable for efficacy and safety considerations, and that direct activation of RGCs will be necessary for reliable activation during high-frequency stimulation.


The Journal of Physiology | 1990

Perceived pitch of vibrotactile stimuli: effects of vibration amplitude, and implications for vibration frequency coding.

John W. Morley; M. J. Rowe

1. The effect of changes in amplitude on the perceived pitch of cutaneous vibratory stimuli was studied in psychophysical experiments designed to test whether the coding of information about the frequency of the vibration might be based on the ratio of recruitment of the PC (Pacinian corpuscle‐associated) and RA (rapidly adapting) classes of tactile sensory fibres. The study was based on previous data which show that at certain vibration frequencies (e.g. 150 Hz) the ratio of recruitment of the PC and RA classes should vary as a function of vibration amplitude. 2. Sinusoidal vibration at either 30 Hz or 150 Hz, and at an amplitude 10 dB above subjective detection thresholds was delivered in a 1 s train to the distal phalangeal pad of the index finger in eight human subjects. This standard vibration was followed after 0.5 s by a 1 s comparison train of vibration which (unknown to the subject) was at the same frequency as the standard but at a range of amplitudes from 2 to 50 dB above the detection threshold. A two‐alternative forced‐choice procedure was used in which the subject had to indicate whether the comparison stimulus was higher or lower in pitch (frequency) than the standard. 3. Marked differences were seen from subject to subject in the effect of amplitude on perceived pitch at both 30 Hz and 150 Hz. At 150 Hz, five out of the eight subjects reported an increase in pitch as the amplitude of the comparison vibration increased, one experienced no change, and only two experienced the fall in perceived pitch that is predicted if the proposed ratio code contributes to vibrotactile pitch judgements. At 30 Hz similar intersubject variability was seen in the pitch‐amplitude functions. 4. The results do not support the hypothesis that a ratio code contributes to vibrotactile pitch perception. We conclude that temporal patterning of impulse activity remains the major candidate code for pitch perception, at least over a substantial part of the vibrotactile frequency bandwidth.


Clinical and Experimental Pharmacology and Physiology | 2007

GABAA RECEPTOR EXPRESSION AND INHIBITORY POST‐SYNAPTIC CURRENTS IN CEREBELLAR PURKINJE CELLS IN DYSTROPHIN‐DEFICIENT mdx MICE

Sindy L. L Kueh; Stewart I. Head; John W. Morley

1 Duchenne muscular dystrophy (DMD) is the second most common fatal genetic disease and arises as a consequence of an absence or disruption of the protein dystrophin. In addition to wasting of the skeletal musculature, boys with DMD have a significant degree of cognitive impairment. 2 We show here that there is no difference between littermate control and mdx mice (a murine model of DMD) in the overall expression of the GABAA receptor a1‐subunit, supporting the suggestion that it is the clustering at the synapse that is affected and not the expression of the GABAA receptor protein. 3 We report a significant reduction in both the frequency and amplitude of spontaneous inhibitory post‐synaptic currents in cerebellar Purkinje cells of mdx mice compared with littermate controls, consistent with the reported reduction in the number and size of GABAA receptor clusters immunoreactive for a1‐ and a2‐subunits at the post‐synaptic densities. 4 These results may explain some of the behavioural problems and cognitive impairment reported in DMD.


Brain Research | 2004

Long-term depression is reduced in cerebellar Purkinje cells of dystrophin-deficient mdx mice.

Jennifer L. Anderson; Stewart I. Head; John W. Morley

The mdx (muscular dystrophy X-linked) mouse is a model for human Duchenne muscular dystrophy (DMD) and is characterized by the absence of the cytoskeletal protein dystrophin. Using a cerebellar slice preparation, we show that postsynaptically mediated long-term depression (LTD) is significantly reduced in mdx Purkinje cells, while presynaptically mediated paired-pulse facilitation (PPF) is normal. This disruption of LTD could contribute to the cognitive deficit in boys with Duchenne muscular dystrophy.


PLOS ONE | 2012

Responses of Retinal Ganglion Cells to Extracellular Electrical Stimulation, from Single Cell to Population: Model-Based Analysis

David Tsai; Spencer C. Chen; Dario A. Protti; John W. Morley; Gregg J. Suaning; Nigel H. Lovell

Retinal ganglion cells (RGCs), which survive in large numbers following neurodegenerative diseases, could be stimulated with extracellular electric pulses to elicit artificial percepts. How do the RGCs respond to electrical stimulation at the sub-cellular level under different stimulus configurations, and how does this influence the whole-cell response? At the population level, why have experiments yielded conflicting evidence regarding the extent of passing axon activation? We addressed these questions through simulations of morphologically and biophysically detailed computational RGC models on high performance computing clusters. We conducted the analyses on both large-field RGCs and small-field midget RGCs. The latter neurons are unique to primates. We found that at the single cell level the electric potential gradient in conjunction with neuronal element excitability, rather than the electrode center location per se, determined the response threshold and latency. In addition, stimulus positioning strongly influenced the location of RGC response initiation and subsequent activity propagation through the cellular structure. These findings were robust with respect to inhomogeneous tissue resistivity perpendicular to the electrode plane. At the population level, RGC cellular structures gave rise to low threshold hotspots, which limited axonal and multi-cell activation with threshold stimuli. Finally, due to variations in neuronal element excitability over space, following supra-threshold stimulation some locations favored localized activation of multiple cells, while others favored axonal activation of cells over extended space.


Investigative Ophthalmology & Visual Science | 2013

Current Steering in Retinal Stimulation via a Quasimonopolar Stimulation Paradigm

Paul B. Matteucci; Spencer C. Chen; David Tsai; Christopher W. D. Dodds; Socrates Dokos; John W. Morley; Nigel H. Lovell; Gregg J. Suaning

PURPOSE Research to restore some degree of vision to patients suffering from retinal degeneration is becoming increasingly more promising. Several groups have chosen electrical stimulation of the remaining network of a degenerate retina as a means to generate discrete light percepts (phosphenes). Approaches vary significantly, with the greatest difference being the location of the stimulating electrode itself. METHODS Suprachoroidal positioning offers excellent mechanical stability and surgical simplicity; however, at the cost of activation thresholds and focused stimulation due to the distance from the electrodes to the target neurons. Past studies proposed a hexapolar electrode configuration to focus the cortical activation and minimize cross-talk between electrodes during concurrent stimulation. The high impedance nature of the choroid and pigment epithelium, however, cause current to shunt between the stimulating and return electrodes, resulting in even higher activation thresholds. In our study, we analyzed the effect of stimulating the feline retina using a quasimonopolar stimulation by simultaneously stimulating a hexapolar and distant monopolar return configurations. RESULTS Results of in vivo studies showed that quasimonopolar stimulation can be used to maintain the activation containment properties of hexapolar stimulation, while lowering the activation threshold to values almost equivalent to those of monopolar stimulation. CONCLUSIONS The optimal stimulus was found to be composed of a subthreshold monopolar stimulus combined with a suprathreshold hexapolar stimulation. This resulted in a decrease of activation threshold of 60% with respect to hexapolar alone, but with no discernible deleterious effect on the charge containment of a pure hexapolar stimulation.


Brain Research | 2003

Altered inhibitory input to Purkinje cells of dystrophin-deficient mice

Jennifer L. Anderson; Stewart I. Head; John W. Morley

We investigated evoked EPSPs and spontaneous IPSPs in cerebellar slices from dystrophin-deficient mdx mice. In the presence of the GABA(A) antagonist bicuculline the increase in EPSP amplitude was less in mdx Purkinje cells compared to control, and the amplitude of miniature IPSCs in mdx cells was also significantly less than in controls. This reduced inhibitory input is most likely due to the reported reduction in the size of GABA(A) channel clusters.


The Journal of Physiology | 1997

Spatial and temporal frequency selectivity of cells in area 21a of the cat.

John W. Morley; Richard M. Vickery

1 The spatial and temporal response properties of single cells in area 21a of the anaesthetized cat were assessed using drifting sinusoidal gratings presented at the optimum orientation for each cell. 2 Responses to sinusoidal gratings were dominated by an elevation of the mean discharge, with a relatively small modulated component at the temporal frequency of grating drift. The relative modulation ratio for the majority of cells was less than 1, similar to complex cells in the striate cortex. 3 Of those cells responsive to stimulation with sinusoidal gratings, 94% displayed spatial bandpass characteristics. Values derived from spatial frequency tuning curves were: mean optimum spatial frequency, 0.26 cycles deg−1; mean spatial resolution, 0.86 cycles deg−1; mean spatial bandwidth, 1.8 octaves; and mean normalized bandwidth, 1.3. Two cells (6%) displayed spatial low‐pass characteristics. 4 Approximately half our sample of cells (44%) displayed temporal low‐pass tuning, while 35% displayed temporal bandpass characteristics. The mean optimum temporal frequency of bandpass cells was 3.3 Hz and the mean temporal bandwidth 1.9 octaves. The remaining cells were classified as temporal broadband (17%) and temporal high‐pass (4%). 5 We conclude that the dominant functional input to cells with relatively high spatial frequency selectivity and/or temporal low‐pass response properties most probably arises from area 17. The responses of the remaining cells may be explained by input from area 17 or 18.


Journal of Neural Engineering | 2011

Activation of retinal ganglion cells following epiretinal electrical stimulation with hexagonally arranged bipolar electrodes

Miganoosh Abramian; Nigel H. Lovell; John W. Morley; Gregg J. Suaning; Socrates Dokos

We investigated retinal ganglion cell (RGC) responses to epiretinal electrical stimulation delivered by hexagonally arranged bipolar (Hex) electrodes, in order to assess the feasibility of this electrode arrangement for future retinal implant devices. In vitro experiments were performed using rabbit retinal preparations, with results compared to a computational model of axonal stimulation. Single-unit RGC responses to electrical stimulation were recorded with extracellular microelectrodes. With 100 µs/phase biphasic pulses, the threshold charge densities were 24.0 ± 11.2 and 7.7 ± 3.2 µC cm(-2) for 50 and 125 µm diameter Hex electrodes, respectively. Threshold profiles and response characteristics strongly suggested that RGC axons were the neural activation site. Both the model and in vitro data indicated that localized tissue stimulation is achieved with Hex electrodes.

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Nigel H. Lovell

University of New South Wales

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Gregg J. Suaning

University of New South Wales

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David Tsai

University of New South Wales

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Socrates Dokos

University of New South Wales

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Spencer C. Chen

University of New South Wales

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Tianruo Guo

University of New South Wales

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Stewart I. Head

University of New South Wales

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Richard M. Vickery

University of New South Wales

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Minas T. Coroneo

University of New South Wales

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Antonio Lauto

University of Western Sydney

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