Jong Soo Jurng

Electrospray-assisted ultraviolet aerodynamic particle sizer spectrometer for real-time characterization of bacterial particles.

The ultraviolet aerodynamic particle sizer (UVAPS) spectrometer is a novel, commercially available aerosol counter for real-time, continuous monitoring of viable bioaerosols based on the fluorescence induced from living microorganisms. For aerosolization of liquid-based microorganisms, general aerosolization methods such as atomization or nebulization may not be adequate for an accurate and quantitative characterization of the microorganisms because of the formation of agglomerated particles. In such cases, biological electrospray techniques have an advantage because they generate nonagglomerated particles, attributable to the repulsive electrical forces among particles with unipolar charges. Biological electrosprays are quickly gaining potential for the detection and control of living organisms in applications ranging from mass spectrometry to developmental microbiology. In this study, we investigated the size distribution, total concentration, and fluorescence percentage of bacterial particles in a real-time manner by electrospray-assisted UVAPS. A suspension containing Escherichia coli as a test microorganism was sprayed in a steady cone-jet mode using a specially designed electrospray system with a point-to-orifice-plate configuration based on charge-reduced electrospray size spectrometry. With the electrospray process, 98% of the total E. coli particle number concentration had a size of <1 mum and the geometric mean diameter was 0.779 mum, as compared with the respective values of 78% and 0.907 mum after nebulization. The fractions of fluorescence responsive particles and of particles that contained viable organisms in culture were 12% and 7%, respectively, from the electrospray process and 34% and 24% from nebulization. These results demonstrate that (1) the presence of agglomerated particles can lead to markedly overestimated fluorescence and culturability percentages compared with the values obtained from nonagglomerated particles, and (2) electrospray-assisted UVAPS can provide more accurate and quantitative real-time characterization of liquid-based microorganisms, owing to the generation of nonagglomerated particles.

Flow Analysis of Engine Cooling System for a Passenger Vehicle

A numerical simulation is carried out to analyze the flow field of cooling air through the radiator and engine compartment. In order to consider the strong effect of the suction-type flow by the cooling fan at engine idling condition, a potential flow analysis is attempted by the assumption of a line sink located at the position of the cooling fan. The governing equations for steady two-dimensional, incompressible, turbulent flow are solved with the two-equationk-ε model for turbulence. The velocity profiles in the underhood engine compartment and around the front-end of a real vehicle are measured to compare with the numerical results. The agreement between the numerical and experimental results is fairly good. It is concluded that a two-dimensional computation is a fast and efficient tool for predicting the effect of front-end design on the cooling air flow through the radiator.

A study on the pressure loss, heat transfer enhancement and fouling control in a vertical particulate flow

In this study, the pressure loss, the heat transfer rate and the fouling characteristics of a particulate flow were investigated. Particles used were the glass beads of 3.0 mm diameter with 2.54 specific gravity. The particles augmented the heat transfer at the flow velocities lower than 1.0 m/s. In this range, the heat transfer coefficient slighly increased as the particle volume fraction increased, and was almost independent of the flow velocity. The particles also increased the pressure loss at the flow velocities lower than 1.0 m/s. Above 1.0 m/s, however, the heat transfer coefficient and the pressure loss were essentially the same as those of flow with pure water. Through the flow visualization study, the collision frequency on the wall by particles is shown to be closely related with the heat transfer enhancement. The particles effectively controlled fouling. Fouling tests using ferric oxide revealed that the particles effectively removed the pre-exsisting deposit as well as they prevented the deposit buildup.