José Alexandre Bachur

The effects of high-intensity resistance exercise on the blood lipid profile and liver function in hypercholesterolemic hamsters

It is well established that atherogenic dyslipidemia, characterized by high levels of triglycerides (TG), total cholesterol (TC), and low-density lipoprotein (LDL) cholesterol and low levels of high-density lipoprotein (HDL) cholesterol, constitutes important risk factors for cardiovascular disease. Regular exercise has been associated with a reduced risk for metabolic diseases. However, studies supporting the concept that resistance exercise is a modifier of blood lipid parameters are often contradictory. The aim of this study was to investigate the effects of high-intensity resistance exercise on the serum levels of TG, TC, HDL and non-HDL cholesterol, glucose, and the liver function enzymes alanine aminotransferase (ALT, EC 2.6.1.2) and aspartate aminotransferase (AST, EC 2.6.1.1) in golden Syrian hamsters (Mesocricetus auratus (Waterhouse, 1839)) fed a hypercholesterolemic diet. Sedentary groups (S) and exercise groups (E) were fed a standard diet (SS and ES) or a cholesterol-enriched diet (standard plus 1% cholesterol, SC and EC). Resistance exercise was performed by jumps in the water, carrying a load strapped to the chest, representing 10 maximum repetitions (10 RM, 30 s rest, five days per week for five weeks). Mean blood sample comparisons were made by ANOVA + Tukey or ANOVA + Kruskal-Wallis tests (p < 0.05) to compare parametric and nonparametric samples, respectively. There were no differences in blood lipids between the standard diet groups (SS and ES) (p > 0.05). However, the EC group increased the glucose, non-HDL, and TC levels in comparison with the ES group. Moreover, the EC group increased the TG levels versus the SC group (p < 0.05). In addition, the ALT levels were increased only by diet treatment. These findings indicated that high-intensity resistance exercise contributed to dyslipidemia in hamsters fed a hypercholesterolemic diet, whereas liver function enzymes did not differ in regards to the exercise protocol.

Avaliação do efeito da hipotermia por crioimersão corporal, nos neutrófilos e linfócitos sanguíneos de ratos submetidos ao exercício físico agudo

Systemic stress induced by exercise increases bioactive substances in plasma which leads to neutrophilic mobilization. Cryotherapy causes a decrease in the inflammatory reaction and attenuates high blood perfusion after exercise. The objective of this work was to analyze the influence of cold water immersion (CWI) after acute exercise on neutrophil and lymphocyte mobilization. A control group of rats (AI) was kept at rest and a second group (AII) was submitted to CWI at 10o C for 10 minutes. The animals of Groups BI, BII, BIII and BIV were submitted to acute exercise which consisted in swimming in water at 31o C for 100 minutes with a load equivalent to 5% of the body weight. Groups CI, CII, CIII and CIV were submitted to CWI immediately after acute exercise. The animals were sacrificed at 6 (I), 12 (II), 24 (III) and 48 (IV) hours after the exercise and neutrophil and lymphocyte cells were counted for all groups by optic microscopy. The Student t-test was used for statistical analysis with a significance level of p< 0.05. A significant increase in the number of neutrophils was observed in Groups AII, BI, BII, BIII, BIV, CI, CII and CIII compared to AI. The neutrophil count of the CIV Group was similar to the Control Group. There was a significant drop in the number of lymphocytes in Groups BII, BIII, BIV, CI and CII when compared to Group AI. The lymphocyte count of Groups AII, BI, CIII and CIV were similar to the Control Group. The changes in neutrophil and lymphocyte counts caused by acute exercise were reverted to normal at 24 hours by cold water immersion.

Estresse oxidativo no plasma sanguíneo de indivíduos submetidos ao esforço físico agudo seguido de crioimersão corporal

O objetivo deste estudo foi analisar a influencia da crioimersao corporal (CIC) imediata ao esforco fisico agudo no estresse oxidativo (EOx) no plasma sanguineo. Participaram do presente estudo 12 homens, com idade media de 22±1 anos, submetidos ao teste de esforco fisico intenso em esteira, seguido de CIC em um tanque com agua a 10oC durante 10 minutos continuos. Do repouso ao final da CIC, os individuos foram monitorados atraves de alguns parâmetros como: o indice de percepcao subjetiva do esforco (IPE) expresso conforme escala de Borg, frequencia cardiaca (FC), pressao arterial (PA) e temperatura corporal (TC) atraves da temperatura timpânica. A analise morfologica do EOx plasmatico foi realizada de acordo com o metodo denominado Morfologia Optica do Estresse Oxidativo no Plasma (MEOP), utilizando-se gotas de sangue capilar. Observou-se uma significativa elevacao (p<0,01) no grau do estresse oxidativo plasmatico apos a realizacao do esforco fisico, em relacao ao respectivo grau em repouso. Porem, esta elevacao no grau do EOx foi significativamente reduzida (p<0,001) em funcao da CIC. Embora sejam necessarios mais estudos cientificos com o MEOP, concluiu-se que, para o presente estudo, este teste mostrou-se viavel. Os dados encontrados no presente estudo sugerem que a CIC em agua a 10oC por 10 minutos imediatos ao esforco fisico agudo com intensidade alta, apresenta-se como uma importante conduta fisioterapeutica para a normalizacao do EOx pos-esforco.

Efeito da cinesioterapia na lesão isquêmica e reperfusão em ratos

Objective To investigate the effect of kinesiotherapy on the functionality of the pelvic limb of rats after ischemic and reperfusion injury. Methods 10 rats were divided into two groups, GI (control) and GII (kinesiotherapy). All the animals underwent ischemia for a period of three hours, followed by tissue reperfusion. In Group GII, non-resistive systemic kinesiotherapy was performed (swimming) in three weekly sessions of 50 minutes, over a period of four weeks, while the GI animals remained at rest. Functional analysis of motor behavior was evaluated weekly. The animals were then sacrificed, and the soleus and gastrocnemius muscles and the sciatic nerve removed for histopathological analysis. Results There was a significant recovery of motor behavior with kinesiotherapeutic treatment during the four weeks of treatment. However, the histological examination of the tissues showed no morphological changes of cell injury and repair. Conclusion It was not possible to affirm that the exercise was effective in cell repair, because neither of the groups (control and experimental) showed any histological difference. On the other hand, systemic kinesiotherapy showed a beneficial effect on functional rehabilitation after ischemia and reperfusion. Level of evidence III, Case-Control Study.OBJECTIVE: To investigate the effect of kinesiotherapy on the functionality of the pelvic limb of rats after ischemic and reperfusion injury. METHODS: 10 rats were divided into two groups, GI (control) and GII (kinesiotherapy). All the animals underwent ischemia for a period of three hours, followed by tissue reperfusion. In Group GII, non-resistive systemic kinesiotherapy was performed (swimming) in three weekly sessions of 50 minutes, over a period of four weeks, while the GI animals remained at rest. Functional analysis of motor behavior was evaluated weekly. The animals were then sacrificed, and the soleus and gastrocnemius muscles and the sciatic nerve removed for histopathological analysis. RESULTS: There was a significant recovery of motor behavior with kinesiotherapeutic treatment during the four weeks of treatment. However, the histological examination of the tissues showed no morphological changes of cell injury and repair. CONCLUSION: It was not possible to affirm that the exercise was effective in cell repair, because neither of the groups (control and experimental) showed any histological difference. On the other hand, systemic kinesiotherapy showed a beneficial effect on functional rehabilitation after ischemia and reperfusion. Level of evidence III, Case-Control Study .

Incidence and Characteristics Angiographic of Patients with Acute Myocardial

Mailing Address: Cynthia Kallás Bachur Rua Alberto de Azevedo, 2020. Postal code: 14405-281, Jardim Califórnia, Franca, SP Brazil. E-mail: cynthia.bachur@unifran.edu.br Incidence and Characteristics Angiographic of Patients with Acute Myocardial Cynthia Kallás Bachur,1,2 José Alexandre Bachur,1 Juliana Pereira Machado,3 Eugenia Velludo Veiga,2 Sarah da Silva Candido,2 Ricardo Barbosa,4 Julia Granado Carraro,1 Danielle de Freitas Gonçalves,1 Maria Georgina Marques Tonello1 Universidade de Franca,1 Franca, SP Brazil Universidade de São Paulo,2 São Paulo, SP Brazil Centro Universitário Barão de Mauá,3 Ribeirão Preto, SP Brazil Hospital do Coração de Franca,4 Franca, SP Brazil

REPERCUSSÕES FÍSICO-FUNCIONAIS DO AJUSTE ERGONÔMICO EM CICLISTAS TRABALHADORES DA INDÚSTRIA DE CALÇADOS

O ciclismo e frequentemente prejudicado por lesoes por esforco repetitivo que podem estar associadas a um desajuste entre as caracteristicas corporais do ciclista em relacao as dimensoes da bicicleta. Os objetivos do estudo sao: avaliar a eficacia clinica e funcional do ajuste ergonomico especifico ao ciclismo, quantificar o numero de ciclistas regulares com necessidades de ajuste ergonomico especifico, relacionar os sinais e sintomas presentes nestes ciclistas e relaciona-los ao padrao postural de ciclismo adotado usualmente. Serao estudados trabalhadores do setor calcadista que utilizam regularmente a bicicleta como meio de transporte. Sera realizada uma avaliacao do padrao postural corporal ortostatica e na posicao sentado sobre a bicicleta de uso pessoal, seguidas da realizacao do teste ergometrico (TE) de carater submaximo conforme protocolo pre-estabelecido. Serao capturadas imagens do participante para visualizacao e mensuracao dos ângulos de flexao e extensao das articulacoes dos membros, durante as situacoes de posicionamento postural estatico sobre a bicicleta e de realizacao do ato de pedalar. Posteriormente, sera realizado o ajuste da bicicleta, necessario para a adequacao ergonomica. Assim, os participantes deverao realizar cinco atividades ciclisticas de rotina e em seguida comparecer a clinica de fisioterapia da Unifran para a realizacao de um novo TE, para avaliacao dos efeitos da intervencao ergonomica realizada. As analises cinematicas e estatisticas serao realizadas atraves de softwares especificos. Espera-se verificar a elevacao do desempenho acompanhada de reducao dos niveis de dor e cansaco. A autorizacao do Comite de Etica esta sendo aguardada para o inicio dos testes.

Análise das variáveis hemodinâmicas no posicionamento gravitacional

INTRODUCTION: The autonomic nervous system governs the short and medium term, the necessary modifications to adapt blood pressure and heart rate to changes in posture. OBJECTIVE: To analyze the hemodynamics variables in healthy individuals when subjected to positional gravity. MATERIALS AND METHODS: Sixty healthy individuals, divided in groups based on age, being GI (n = 20), from 15 to 20 years old, GII (n = 20), from 21 to 40 years old, GIII (n = 20) from 41 to 60 years old, being submitted to the position gravity through the GPS equipment and they had the cardiac frequency and blood pressure monitored throughout all the time of inversion. RESULTS: A significant reduction of heart rate and blood pressure in the rest position when compared after 3, 6, 9 and 12 minutes of inversion, however 3 minutes after return to the orthostatic position this variables came back near to the baseline. About the blood pressure diastolic, it was not observed a significant decrease in the GI and GII group in the rest position when compared after 3 minutes; however in the GIII group it was observed a significant drop. After 6 minutes of inversion only the GI group showed a significant drop. When analyzed 9 minutes after the position, only the GIII group showed a significant dro. After 12 minutes the inversion, it was also showed a blood pressure diastolic drop only in GIII group when compared to the values of the rest position. Returning to the vertical position, all groups returned to the baseline values after 3 minutes. CONCLUSION: The gravitational positioning changes the hemodynamics variables causing a drop of the values of the heart rate and the blood pressure during the inversion period.