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Dive into the research topics where José Carlos García is active.

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Featured researches published by José Carlos García.


Animal Reproduction Science | 2010

Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility.

José Carlos García García; Jc Dominguez; F.J. Peña; B Alegre; R. Gonzalez; Mj Castro; G.G. Habing; Roy N. Kirkwood

Causes of poor fertility after insemination of frozen-thawed (FT) sperm include reduced sperm viability at thawing and a shorter longevity of surviving sperm in the female genital tract due to sub-lethal damage. The present studies examined the effect of incubating thawed boar sperm in seminal plasma (SP) on sperm membrane integrity (viability), and motility in vitro (experiment 1), and fertility in vivo (experiment 2). For experiment 1, FT sperm from five individual boars and a sperm pool from these boars were thawed and incubated for 4 h in media containing 0%, 10%, or 50% autologous seminal plasma (individual boars) or pooled seminal plasma (sperm pool). At approximately 10 min (0 h) and again at 1 h, 2 h, 3 h, and 4 h, sperm populations were examined for percentage sperm viability and percent sperm motility. Each variable progressively decreased during the incubation period. Incubation in 50% SP increased percentages of live sperm (P < 0.0001) and percent sperm motility (P < 0.01) at all time points compared to incubation in either 0% or 10% SP. For experiment 2, multiparous Large white x Landrace sows (n = 82) each received 900 IU eCG at weaning and 750 IU hCG 80 h later to control time of ovulation. Sows were assigned on the basis of parity to be inseminated with pooled semen with or without SP from the boars used in experiment 1. Sows received 3 x 10(9) live fresh-extended sperm (n = 30) or FT sperm thawed in 80 mL BTS extender (n = 26) or 3 x 10(9) live FT sperm thawed in 80 mL BTS containing 50% SP (FT-SP; n = 26). Sows were inseminated at 36 h, and 42 h after hCG injection. Compared to sows receiving fresh semen, the pregnancy rate of FT inseminated sows tended (P = 0.06) to be lower with the FT-SP group being intermediate. Farrowing rates were not different (83.3%, 69.2%, and 65.4% for fresh, FT, and FT-SP, respectively). Inseminations with FT sperm were associated with a reduction in litter size (P < 0.05), which was not evident in the FT-SP group. Taken together, these data confirm an adverse effect of inseminating FT sperm on sperm quality and sow fertility but suggest that thawing FT sperm in 50% SP may partially alleviate these adverse effects.


Journal of Animal Science | 2010

Effect of gonadotropin treatment on estrus, ovulation, and litter size in weaned and anestrous sows

R. Manjarin; José Carlos García García; Jc Dominguez; Mj Castro; B Alegre; J. D. Munoz; Roy N. Kirkwood

In the first of 2 experiments, we evaluated the effects on anestrous sows of pretreatment with FSH to stimulate the growth of small follicles, followed by eCG to stimulate the growth of medium follicles, estrus, and ovulation. In Exp. 2, we examined the effect of sows receiving 400 IU of eCG plus 200 IU of hCG (PG 600, Intervet/Schering Plough Animal Health, Boxmeer, the Netherlands) at weaning and then different doses and timing of supplemental hCG. In Exp. 1, a total of 87 multiparous Hypor sows deemed anestrus 7 d after weaning were assigned to intramuscular (i.m.) injection of 1) PG 600, 2) eCG (600 IU), 3) pretreatment with 87.5 IU of FSH on d 7 and 8 plus eCG on d 9, or were 4) noninjected controls. Sows had daily boar contact for 15 d after weaning for estrus detection. Blood samples were obtained on d 9 and 19 and assayed for progesterone to determine ovulation status. The weaning-to-estrus interval, number of sows in estrus and ovulating, farrowing rate, and litter size were not different (P > 0.1) in treated groups compared with controls. In Exp. 2, a total of 247 Hypor sows were assigned at weaning by parity (1 and 2 or > or = 3) to receive 1) an i.m. injection of PG 600, 2) PG 600 supplemented with 100 IU of hCG injected either concurrently or after 24 h, 3) 200 IU of hCG after 24 h, or 4) no injection (controls). Sows were exposed to boars daily for 7 d. After treatment of parity 1 and 2 sows, all gonadotropin-treated groups had an increased (P < 0.05) number of sows in estrus compared with the control group; weaning-to-estrus interval, farrowing rates, and litter size were unaffected (P > 0.1). After treatment of parity > or = 3 sows, there was no treatment effect on the estrous response and weaning-to-estrus interval; compared with control and PG 600-treated sows, farrowing rate was decreased (P < 0.05) for sows receiving 200 IU of hCG after 24 h. There was no effect (P > 0.1) of treatment on litter size. We conclude that gonadotropins can be used to increase estrus response in weaned sows, but that hCG treatment subsequent to PG 600 may be detrimental to sow fertility in parity > or = 3 sows.


Suis | 2017

Avances en la criopreservación del semen porcino: mejora de la congelabilidad y de los rendimientos del proceso

Beatriz Alegre Gutiérrez; Christina Gómez; José Carlos García García; R. Manjarin; M. Abad; Juan Carlos Domínguez Fernández de Tejerina


Suis | 2017

Avances en la criopreservación del semen porcino: situación actual y factores limitantes

Beatriz Alegre Gutiérrez; Christina Gómez; José Carlos García García; R. Manjarin; M. Abad; Juan Carlos Domínguez Fernández de Tejerina


Suis | 2017

Avances en la criopreservación de semen porcino: selección de verracos y avances en los protocolos de inseminación artificial

Beatriz Alegre Gutiérrez; Christina Gómez; José Carlos García García; R. Manjarin; M. Abad; Juan Carlos Domínguez Fernández de Tejerina


MG Mundo ganadero | 2015

Adicción de plasma seminal para la mejora del semen criopreservado de porcino

Juan Carlos Domínguez Fernández de Tejerina; Beatriz Alegre Gutiérrez; José Carlos García García; M. Abad; Roy N. Kirkwood; D. Martín-Hidalgo; Rodrigo Manjarín Arredondo; Christina Gómez


Canadian Journal of Veterinary Research-revue Canadienne De Recherche Veterinaire | 2015

Effect of additional human chorionic gonadotrophin (hCG) on follicular growth and ovulation in gonadotrophin-treated gilts.

R. Manjarin; Glen Cassar; Robert M. Friendship; José Carlos García García; J. Carlos Dominguez; Roy N. Kirkwood


Albéitar: publicación veterinaria independiente | 2010

La fluorquinolona y la vitalidad del semen

Juan Carlos Domínguez Fernández de Tejerina; Raúl González Urdiales; Beatriz Alegre Gutiérrez; José Carlos García García; Claudia Cordova; Ricardo Fernandez; D. Martín-Hidalgo


Suis | 2009

Dirección de centros de inseminación artificial porcina (1ª parte): principios generales de dirección y gestión de centros de inseminación artificial porcina

Juan Carlos Domínguez Fernández de Tejerina; Humberto Osvaldo Cisale; Beatriz Alegre Gutiérrez; R. González; José Carlos García García; Fernando Tejerina Ampudia; Sergio Eduardo Bernal García; Sonia Marcela Cárdenas Hernández; M. Abad; Francisco Abad Valcarce


Suis | 2009

Dirección de centros de inseminación artificial porcina 4ª parte:: Control de la gestión del CIAP

Juan Carlos Domínguez Fernández de Tejerina; Beatriz Alegre Gutiérrez; R. González; José Carlos García García; Fernando Tejerina Ampudia

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R. Manjarin

Michigan State University

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Claudia Cordova

Spanish National Research Council

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F.J. Peña

University of Extremadura

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G.G. Habing

Michigan State University

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