José Maciel Caldas dos Reis

Níveis séricos de aminotransferases, bilirrubinas e gama-glutamil transpeptidase após a admininstração de óleo de copaíba em ratos

Copaiba oil is a oil-resin used as a phytotherapic in Amazon region in a folkloric way. This use is empirical and little is known about its effect in the organic systems. Objective: To determine serum levels of aminotransferases, bilirrubins and gama-glutamyl transpeptidase after copaiba oil administration. Methods: Male Wistar rats (n=20) divided in two groups of 10 animals: Group Cop which received a daily oral administration of copaiba oil, 0.63ml/kg, during 5 days; Group P in which any substance was administrated. In the sixth day the animals of both groups were submitted to inalatory anesthesia, laparotomy and collection of blood samples (3ml) from inferior vena cava for the determination of serum aminotransferases, bilirrubins and gama-glutamyl transpeptidase levels. Results: The results were compared between groups and analyzed by t-Student test. Group Cop showed AST, ALT and GGT levels significantly decreased when compared with Gruop P (p<0.01), while total bilirrubins levels increased by its direct fraction. Conclusion: Copaiba oil can alter serum levels of aminotransferases, bilirrubins and gama-glutamyl transpeptidase, showing a possible interaction between this substance and liver, but this considerations require posterior evalutaion.

Lesão intestinal após isquemia-reperfusão: estudo comparativo usando sal tetrazólico (MTT) e histologia

Varios metodos sao utilizados para avaliar e estimar as lesoes intestinais de isquemia e reperfusao (IR). Assim, o objetivo do presente trabalho e realizar o estudo comparativo dos aspectos colorimetrico e histologico da lesao intestinal apos IR. Para tal, foram utilizados 30 ratos Wistar, machos, pesando entre 310 a 410g, distribuidos em 3 grupos: Grupo Controle (GC), Grupo Isquemia e Reperfusao-1 (GIR-1) e Grupo Isquemia e Reperfusao-3 (GIR-3), com 10 animais cada. Nos grupos GIR-1 e GIR-3 foi realizada isquemia intestinal, por meio de falsa ligadura da arteria mesenterica anterior, durante 30 minutos e apos esta a perfusao sanguinea foi restaurada. Estes animais foram submetidos a eutanasia apos 1 e 3 dias de reperfusao, respectivamente, sendo colhido material para realizacao dos estudos colorimetrico, usando o Methyl Thiazolyl Blue (MTT) e histologico pela hematoxilina e eosina. Os resultados obtidos demonstraram uma menor proporcao de celulas viaveis e um maior grau de lesao da tunica mucosa nos animais do grupo GIR-3 em relacao ao controle (p<0,05). Desta forma os autores concluem que o estudo colorimetrico, usando o MTT, mostrou-se tao eficaz e confiavel quanto o estudo histologico na avaliacao das repercussoes intestinais produzidas pela IR deste orgao.

Aspectos microscópicos do fígado de ratos após administração do óleo de copaíba

The use of medicinal plants is a commonly practice observed at developing countries. Then, this paper aim to verify the effect of the copaiba oil in the liver of rats. Fifty male Wistar rats were used distributed into five groups: Group Copaiba 0.63 (GC0.63 ml/kg of copaiba oil), Group Copaiba 0.06 (GC0.06 ml/kg of copaiba oil), Group Water (GA0.63 ml/kg of water), Group Corn Oil (GM0.63 ml/kg of corn oil) and Standard group (GP). The substances were administered daily by 14 days. The animals were submitted to euthanasia in the 14th day, being withdraw the liver and realized histologic study. The results demonstrated that microscopically, in the hepatocity cells no alterations were found; however, vascular congestion was verified in the portal and mid-lobular veins. A larger congestion was observed in the group GC0.63 in relation to the groups GC0.06, GM, GA and GP (p 0.05), suggesting a dose-effect relationship. Then, the authors conclude that in agreement with methodology used, the copaiba oil promote vascular congestion in the liver of rats, suggesting dose-dependent action.

Do the femoral veins of female Wistar rats have valves

The femoral veins of 30 female rats of the Wistar lineage were studied using histological methods with the objective of determining whether they have valves. Histological analysis did not detect any endothelial projections or valve recesses that would suggest the presence of venous valves in this species of animal.