Juhani Sibakov

Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.

The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied. Free amino acid (FAA) profile analysis was also carried out. Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures. Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed. The anti-nutritional compounds were separated mostly to the fine protein-rich fraction. Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction). No significant (P>0.05) variation was observed for total phenols and phytic acid content. Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index. This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.

Effect of a milling pre-treatment on the enzymatic hydrolysis of carbohydrates in brewer's spent grain.

Millions of tonnes of brewers spent grain (BSG) are annually produced worldwide as a by-product of the brewing industry. BSG has the potential to be a valuable source of food, chemicals and energy if cost-efficient fractionation methods can be developed. A 2-fold improvement in carbohydrate solubilisation could be achieved through the introduction of a milling step prior to enzymatic hydrolysis. Course and fine milled fractions were characterized by particle size distribution and light microscopy. Fine milling decreased particle size down to the micron level and this in turn improved the carbohydrate solubility yield by a multi-enzyme mixture from 23% up to 45%. Carbohydrate solubilisation could be further increased through the supplementation of this enzyme preparation with additional cellulases. The physical degradation caused by the milling also liberated soluble carbohydrates without the requirement of any enzymatic treatment.

The food matrix affects the anthocyanin profile of fortified egg and dairy matrices during processing and in vitro digestion

The aim of the present study was to understand to what extent the inclusion of anthocyanins into dairy and egg matrices could affect their stability after processing and their release and solubility during digestion. For this purpose, individual and total anthocyanin content of four different enriched matrices, namely custard dessert, milkshake, pancake and omelettete, was determined after their manufacturing and during in vitro digestion. Results showed that anthocyanin recovery after processing largely varied among matrices, mainly due to the treatments applied and the interactions developed with other food components. In terms of digestion, the present study showed that the inclusion of anthocyanins into food matrices could be an effective way to protect them against intestinal degradation, and also the incorporation of anthocyanins into matrices with different compositions and structures could represent an interesting and effective method to control the delivery of anthocyanins within the different compartments of the digestive tract.

Cereal brans as dietary fibre ingredients

This chapter reviews the technologies for production and use of wheat, rye, oat, barley and rice bran. These important ingredients increase the grain fibre content of cereal-based foods in particular. Wheat bran, a co-product of milling wheat into flour, is a commodity with a long history of use as an ingredient. Even though the commercial use pattern of oats, rye and barley is different from that of wheat in the sense that they are more often consumed as wholegrain products, their brans, as well as rice bran, are also commercially available as milling side streams. All brans have their specific composition, functional properties and flavour, and, in addition to providing fibre, assist in diversifying the grain based product portfolio.

Bilberry and bilberry press cake as sources of dietary fibre

Background Dietary recommendations for Nordic countries urge the use of plant foods as a basis for healthy nutrition. Currently, the level of dietary fibre (DF) intake is not adequate. Berries are an elementary part of the recommended Nordic healthy diet and could be consumed in higher amounts. Materials and methods Finnish bilberries and a bilberry press cake from juice processing were studied for DF content, carbohydrate composition, and non-carbohydrate fibre content, which was analysed as sulphuric acid insoluble and soluble material. The microstructure of all samples was also studied using light microscopy and toluidine blue O, calcofluor, and acid fuchsin staining. Results The total DF contents of fresh and freeze-dried bilberries and the press cake were 3.0, 24.1, and 58.9%, respectively. Most of the DF was insoluble. Only about half of it was carbohydrate, the rest being mostly sulphuric acid–insoluble material, waxy cutin from skins, and resilient seeds. Bilberry seeds represented over half of the press cake fraction, and in addition to skin, they were the major DF sources. Microscopy revealed that skins in the press cake were intact and the surface of the seeds had thick-walled cells. Conclusions Bilberry press cake is thus a good source of insoluble non-carbohydrate DF, and could be used to provide DF-rich foods to contribute to versatile intake of DF.

Sourdough and Cereal Beverages

Cereal-based fermented beverages are traditionally used as staple foods and consumed in certain geographical regions. One of the main advantages of the fermented products is the increased mineral availability due to the phytate elimination during fermentations. Further development of the traditional beverage fermentations at an industrial scale could improve the nutritional quality of the products as well as their sensory properties. This chapter focuses on traditional cereal-based non-alcoholic fermented beverages and introduces products that differ in their manufacturing concepts. The microbial composition and manufacturing processes of the beverages as well as their functions are presented in a concise manner. The chapter deals with the following beverages: boza, togwa, mahewu, bushera, pozol, chicha, kishk, kvass, sourish shchi, and hulu-mur. The cereal raw materials for these include: sorghum, finger millet, millet, wheat, maize, rice, rye, oat, and malts.