Julien Mainguy

ARE BODY CONDITION AND REPRODUCTIVE EFFORT OF LAYING GREATER SNOW GEESE AFFECTED BY THE SPRING HUNT

Abstract A spring hunt was implemented on the staging areas of Greater Snow Geese (Chen caerulescens atlantica) in Quebec in 1999 and 2000. We evaluated whether this activity, which occurred during the period of spring nutrient storage, may have affected the body condition and reproductive effort of laying geese. We collected laying females in years with a spring hunt (1999–2000, n = 34) and compared them with birds collected in years without a hunt (1989–1990, n = 10). All indices of body condition and clutch size were significantly lower in years with a hunt than in years without, and laying dates were delayed. Tracking of radio-marked females on the staging and breeding areas showed that a lower proportion of females reached the nesting areas in years with a hunt (28% in 1999–2000, n = 80) than in years without (85% in 1997–1998, n = 80) and that fewer females nested (9% vs. 56%, respectively). Our results suggest that the spring hunt negatively affected nesting geese. ¿Son Afectadas por la Cacería de Primavera la Condición Física y el Esfuerzo Reproductivo de Chen caerulescens atlantica? Resumen. La cacería de primavera fue implementada en áreas de escala de Chen caerulescens atlantica en Quebec en 1999 y 2000. Evaluamos si la cacería, que se llevó a cabo durante el período primaveral de acumulación de nutrientes, pudo haber afectado la condición fisica y el esfuerzo reproductivo de los gansos. Colectamos hembras que estuvieran poniendo huevos en años con cacería de primavera (1999–2000, n = 34) y las comparamos con aves colectadas en años sin cacería (1989–1990, n = 10). Todos los índices de la condición física y del tamaño de la nidada fueron significativamente menores en años con cacería que en años sin cacería, y se retrasaron las fechas de puesta. El seguimiento de hembras marcadas con radio en áreas de escala y cría mostró que una menor proporción llegó a las áreas de nidificación en años con cacería (28% en 1999–2000, n = 80) que en años sin cacería (85% en 1997–1998, n = 80), y que menos hembras nidificaron (9% vs. 56%, respectivamente). Nuestros resultados sugieren que la cacería de primavera afecta negativamente a los gansos nidificantes.

Father–offspring phenotypic correlations suggest intralocus sexual conflict for a fitness-linked trait in a wild sexually dimorphic mammal

In sexually dimorphic and polygynous mammals, sexual selection often favours large males with well-developed weaponry, as these secondary sexual characters confer advantages in intrasexual competition and are often preferred by females. Little is known, however, about the effects of sexually selected paternal traits on offspring phenotype in wild mammals, especially when considering that shared phenotypic traits and selection can also differ greatly between genders. Here, we conducted molecular parentage analyses in a long-term study population of mountain goats (Oreamnos americanus), an ungulate exhibiting high sexual dimorphism in mass, to first assess the determinants of yearly reproductive success (YRS) in males. We then examined the effects of paternal characteristics on offspring mass at 1 year of age. Paternity was highly skewed, with 9 per cent of 57 males siring 51 per cent of 96 offspring assigned over 12 years. Male YRS increased with age until apparent reproductive senescence at 9 years, but mass was a stronger determinant of siring success than age, horn length or social rank. Mass of sons increased with paternal mass, but the mass of daughters was negatively related to that of their father, a finding consistent with recent theory on intralocus sexual conflict. Because early differences in mass persisted to early adulthood, sex-specific effects of paternal mass can have important fitness consequences, as adult mass is positively linked with reproduction in both sexes. Divergent father–offspring phenotypic correlations may partly explain the maintenance of sexual dimorphism in mountain goats and the large variance observed for this homologous trait within each gender in polygynous mammals.

Low MHC DRB class II diversity in the mountain goat: past bottlenecks and possible role of pathogens and parasites

Major histocompatibility complex (MHC) genes are the most polymorphic in vertebrates and code for molecules playing a central role in pathogen resistance. We studied levels of MHC DRB class II diversity in a long-term study population of mountain goats (Oreamnos americanus) at Caw Ridge, Alberta, and two other populations from British Columbia, Canada. Only two alleles were found among the three populations sampled. The Caw Ridge population was fixed for one of the two MHC DRB alleles, but this lack of variation did not appear to have affected it negatively because the population doubled over two decades and had no history of any apparent infectious diseases. Past population bottlenecks during Pleistocene glaciations are thought to have been the main factor contributing to the low levels of MHC diversity in mountain goats, a hypothesis supported by our previous work reporting low polymorphism at neutral loci. Additionally, the limited MHC variability in mountain goats may be related to its northern distribution as we found that allelic diversity at MHC DRB class II in wild ungulates decreases with increasing latitude, possibly as a result of low parasite diversity at high latitudes. The low MHC variation in mountain goats and other northern ungulates such as muskoxen (Ovibos moschatus) may expose these species to population outbreaks that could be generated by introduced pathogens or northward shifts in the distribution of pathogens with global climate warming.

Multilocus heterozygosity, parental relatedness and individual fitness components in a wild mountain goat, Oreamnos americanus population

Matings between relatives lead to a decrease in offspring genetic diversity which can reduce fitness, a phenomenon known as inbreeding depression. Because alpine ungulates generally live in small structured populations and often exhibit a polygynous mating system, they are susceptible to inbreeding. Here, we used marker‐based measures of pairwise genetic relatedness and inbreeding to investigate the fitness consequences of matings between relatives in a long‐term study population of mountain goats (Oreamnos americanus) at Caw Ridge, Alberta, Canada. We first assessed whether individuals avoided mating with kin by comparing actual and random mating pairs according to their estimated genetic relatedness, which was derived from 25 unlinked polymorphic microsatellite markers and reflected pedigree relatedness. We then examined whether individual multilocus heterozygosity H, used as a measure of inbreeding, was predicted by parental relatedness and associated with yearling survival and the annual probability of giving birth to a kid in adult females. Breeding pairs identified by genetic parentage analyses of offspring that survived to 1 year of age were less genetically related than expected under random matings. Parental relatedness was negatively correlated with offspring H, and more heterozygous yearlings had higher survival to 2 years of age. The probability of giving birth was not affected by H in adult females. Because kids that survived to yearling age were mainly produced by less genetically related parents, our results suggest that some individuals experienced inbreeding depression in early life. Future research will be required to quantify the levels of gene flow between different herds, and evaluate their effects on population genetic diversity and dynamics.

Mating tactics and mate choice in relation to age and social rank in male mountain goats

Abstract In polygynous mammals, mating success of males often depends on intense male–male competition and the use of alternative mating tactics. Because reproduction incurs substantial energetic costs and risks of fight injuries, mate selection by males should be expected, particularly when females vary in their ability to produce offspring but can only be defended 1 at a time. Here, we investigated during 3 ruts how age and social rank of male mountain goats (Oreamnos americanus) affected the formation of consort pairs with females (“tending” tactic) in a marked population at Caw Ridge, Alberta, Canada. Among consort pairs, we quantified the behaviors of males and females, and the use of an alternative mating tactic by competing males, “coursing,” which consists of disrupting the pair to gain temporarily access to the female, often by pursuing her. Mate choice was assessed by testing if old and dominant males observed in consort pairs tended experienced females more often than younger females, because reproductive success of females increases with age. Males in consort pairs were ≥4 years old and most (86%, n = 59) were in the top one-half of the dominance hierarchy. Age and social rank of males were positively related to age of females and the total number of young produced by the tended female. All observed matings (n = 32) occurred between 14 November and 2 December and 91% were between males and females in consort pairs. Subordinate males gained mating access to females through coursing, but this tactic was rare. Our study provides evidences of mate choice by males for experienced females in an ungulate and the 1st quantitative information on the rut of mountain goats.

Genetic structure and rabies spread potential in raccoons: the role of landscape barriers and sex-biased dispersal.

Identifying natural barriers to movements of hosts associated with infectious diseases is essential for developing effective control strategies. Raccoon rabies variant (RRV) is a zoonosis of concern for humans because its main vector, the raccoon (Procyon lotor), is found near residential areas. In Québec, Canada, all cases of RRV found in raccoons since 2006 were detected on the eastern side of the Richelieu River, suggesting that this river acts as a barrier to gene flow and thus the potential for RRV to spread. The objectives of this study were to characterize the genetic structure of raccoon populations and assess the effect of the Richelieu River on the population structure in southern Québec, Canada. We also evaluated whether RRV spread potential differed between sex and at a larger spatial scale. Our analyses revealed a weak signal of genetic differentiation among individuals located on each side of the Richelieu River. At a larger spatial scale, genetic structuring was weak. Our results suggest that rivers might not always efficiently restrain raccoon movements and spread of RRV. We suggest that the difference in genetic structure found between sexes can be partly explained by male movements during the breeding season in winter, when ice bridges allow passage over most rivers in Québec.

Temporal dynamics of genetic variability in a mountain goat (Oreamnos americanus) population

The association between population dynamics and genetic variability is of fundamental importance for both evolutionary and conservation biology. We combined long‐term population monitoring and molecular genetic data from 123 offspring and their parents at 28 microsatellite loci to investigate changes in genetic diversity over 14 cohorts in a small and relatively isolated population of mountain goats (Oreamnos americanus) during a period of demographic increase. Offspring heterozygosity decreased while parental genetic similarity and inbreeding coefficients (FIS) increased over the study period (1995–2008). Immigrants introduced three novel alleles into the population and matings between residents and immigrants produced more heterozygous offspring than local crosses, suggesting that immigration can increase population genetic variability. The population experienced genetic drift over the study period, reflected by a reduced allelic richness over time and an ‘isolation‐by‐time’ pattern of genetic structure. The temporal decline of individual genetic diversity despite increasing population size probably resulted from a combination of genetic drift due to small effective population size, inbreeding and insufficient counterbalancing by immigration. This study highlights the importance of long‐term genetic monitoring to understand how demographic processes influence temporal changes of genetic diversity in long‐lived organisms.

Modelling the dispersal of the two main hosts of the raccoon rabies variant in heterogeneous environments with landscape genetics

Predicting the geographic spread of wildlife epidemics requires knowledge about the movement patterns of disease hosts or vectors. The field of landscape genetics provides valuable approaches to study dispersal indirectly, which in turn may be used to understand patterns of disease spread. Here, we applied landscape genetic analyses and spatially explicit models to identify the potential path of raccoon rabies spread in a mesocarnivore community. We used relatedness estimates derived from microsatellite genotypes of raccoons and striped skunks to investigate their dispersal patterns in a heterogeneous landscape composed predominantly of agricultural, forested and residential areas. Samples were collected in an area covering 22 000 km2 in southern Québec, where the raccoon rabies variant (RRV) was first detected in 2006. Multiple regressions on distance matrices revealed that genetic distance among male raccoons was strictly a function of geographic distance, while dispersal in female raccoons was significantly reduced by the presence of agricultural fields. In skunks, our results suggested that dispersal is increased in edge habitats between fields and forest fragments in both males and females. Resistance modelling allowed us to identify likely dispersal corridors used by these two rabies hosts, which may prove especially helpful for surveillance and control (e.g. oral vaccination) activities.

GOSLING GROWTH AND SURVIVAL IN RELATION TO BROOD MOVEMENTS IN GREATER SNOW GEESE (CHEN CAERULESCENS ATLANTICA)

Abstract Post-hatch brood movements to high-quality foraging sites are common in precocial birds but may entail costs for young. We assessed effects of overland movements of broods between the nesting and rearing areas in Greater Snow Geese (Chen caerulescens atlantica) breeding on Bylot Island, Nunavut, Canada, to determine whether these movements affected gosling survival and growth. We monitored 51 radiomarked females over five years to quantify movement distance, movement duration, and gosling survival. Gosling growth was compared over four years using a sample of web-tagged broods recaptured shortly before fledging among adults that (1) nested and reared their young in a dense colony, (2) left the colony and moved ≈30 km to reach the main brood-rearing area, or (3) nested and reared their young in the main brood-rearing area. Brood movements by radio-marked birds were highly variable (2.6-52.5 km, depending on rearing areas used) and fairly rapid (≤6 days after hatch for 72% of the females). Gosling survival was not related to distance moved between nesting and brood-rearing areas. However, gosling growth was influenced by areas used and whether or not they had to move to reach their brood-rearing area. Geese nesting at the main brood-rearing area generally reared heavier and larger goslings than those that moved ≈30 km from the main nesting colony to rear their brood at the main brood-rearing area. On the other hand, goslings leaving the nesting colony after hatch were heavier and larger than those that stayed there throughout brood rearing in one of two years. Although brood movements allow goslings access to high-quality habitats, they entail some costs. Thus, minimizing such movements through nest-site selection should provide a selective advantage by allowing goslings to maximize their growth. Croissance et Survie des Oisons en Relation avec les Déplacements des Familles chez Chen caerulescens atlantica

Genome-wide cross-amplification of domestic sheep microsatellites in bighorn sheep and mountain goats.

We tested for cross‐species amplification of microsatellite loci located throughout the domestic sheep (Ovis aries) genome in two north American mountain ungulates (bighorn sheep, Ovis canadensis, and mountain goats, Oreamnos americanus). We identified 247 new polymorphic markers in bighorn sheep (≥ 3 alleles in one of two study populations) and 149 in mountain goats (≥ 2 alleles in a single study population) using 648 and 576 primer pairs, respectively. Our efforts increased the number of available polymorphic microsatellite markers to 327 for bighorn sheep and 180 for mountain goats. The average distance between successive polymorphic bighorn sheep and mountain goat markers inferred from the Australian domestic sheep genome linkage map (mean ± 1 SD) was 11.9 ± 9.2 and 15.8 ± 13.8 centimorgans, respectively. The development of genomic resources in these wildlife species enables future studies of the genetic architecture of trait variation.