Jun Tae Bae

Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola frondosa

Five groups of polysaccharides were prepared from mycelium extract and top and bottom fraction of filtrate precipitates by submerged culture of Grifola frondosa at two different media (glucose and PMP medium) and their individual biological activities were studied. These polysaccharides had diverse molecular mass (470–1650 kDa) and different biological activities at the concentrations of 0.01–0.2% (w/v). Most of polysaccharides had antioxidant and free radical scavenging activities after UV irradiation, where G-2 (bottom fraction of filtrate precipitates from glucose medium, MW 770 kDa) and G-3 polysaccharide (mycelium extract from glucose medium, MW 500 kDa) showed strong activity. The P-1 (from top fraction of filtrate precipitates from PMP medium, MW 1650 kDa) and P-3 polysaccharide (from mycelium extract from PMP medium, MW 470 kDa) increased the proliferation of fibroblasts by approximately 23–25%. Other two groups of polysaccharides produced from glucose medium (G-2 and G-3 polysaccharides) showed also notable proliferation activity for fibroblasts. Treatment of fibroblasts with P-3 polysaccharide significantly increased the biosynthesis of collagen by approximately 80%. G-2 and G-3 polysaccharides showed also marked activity. However, G-1 and P-1 polysaccharides had only negligible activity in collagen biosynthesis.

Effect of substrate concentration on broth rheology and fungal morphology during exo-biopolymer production by Paecilomyces japonica in a batch bioreactor

The influence of pellet morphology of Paecilomyces japonica on fermentation broth rheology and exo– biopolymer production was investigated. The substrate concentration was varied between 20 and 80 g/liter while running the fermentation at a high aeration of 3 vvm. The specific growth rate of Paecilomyces japonicaand exo– biopolymer production was found to be optimum with a substrate concentration of 40 g/liter of sucrose. Rapid formation of pellets was observed after the first day of fermentation, which slowly increased in size, hairiness and roughness. This, together with the increase in biomass concentration, altered the transport characteristics and the broth rheology to pseudoplastic nature, in turn, influenced exo– biopolymer production. At mild agitation, high aeration and optimum substrate concentration, pellets were the most predominant morphological form compared to free mycelia. Compact pellets with least hairiness and roughness, which improved the flow characteristics of the fermentation broth were observed at 40 g/liter of sucrose and hence regarded as the most productive morphological form.

Optimization of physical parameters for exo-biopolymer production in submerged mycelial cultures of two entomopathogenic fungi Paecilomyces japonica and Paecilomyces tenuipes.

Aims:  In the present study, two different optimization techniques were used to determine the suitable operating parameters for exo–biopolymer production in submerged mycelial cultures of two entomopathogenic fungi Paecilomyces japonica and Paecilomyces tenuipes.

Protective Effects of Fermented Citrus Unshiu Peel Extract against Ultraviolet‐A‐induced Photoageing in Human Dermal Fibrobolasts

The aqueous extracts of Citrus unshiu peel containing flavonoid glycosides was used as co‐substrate with Schizophyllum commune mycelia producing β‐glucosidase and its biological activities were studied. β‐glucosidase‐produced S. commune mycelia converted the glycosides (narirutin and hesperidin) into aglycones (naringenin and hesperetin). The photoprotective potential of fermented C. unshiu peel extract with S. commune (S‐CPE) was tested in human dermal fibroblasts (HDFs) exposed to UVA. It was revealed that S‐CPE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP‐1) expression in UVA‐irradiated HDFs. The treatment of UVA‐irradiated HDFs with S‐CPE resulted in a dose‐dependent decrease in the expression level of MMP‐1 mRNA. The UVA irradiation raised the proportion of senescence‐associated β‐galactosidase (SA‐β‐gal) positive cells in comparison with the normal control group. The treatment of UVA‐irradiated HDFs with S‐CPE was shown to decrease the level of SA‐β‐gal (by approximately 45% at an S‐CPE concentration 0.1%, w/v) compared with the UVA‐irradiated HDFs. It was found that S‐CPE containing hesperetin has notable collagen biosynthetic activity for fibroblasts, indicating that S‐CPE can be promising cosmetic ingredients. Copyright

Production of Flavonoid Compounds and Anti-inflammatory Property of Fermented Licorice Extract with the Basidiomycete Grifola frondosa HB0071

리퀴리티게닌과 이소리퀴리티게닌은 감초의 주요 플라보노이드 성분이다. 이들 플라보노이드는 수용성 감초 추출물과

Study of 2,3,5-Triphenyltetrazolium Chloride for Detection of Pathogenic Microorganisms

{\beta}

Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa

-glucosidase를 생성하는 잎새버섯 HB0071 균사체 발효배양을 통하여 생산하였다. 감초추출물 내 리퀴리티게닌과 이소리퀴리티게닌은 잎새버섯 발효배양 동안 현저히 증가하였다. 이 균주의

Production of exopolysaccharide from mycelial culture of Grifola frondosa and its inhibitory effect on matrix metalloproteinase‐1 expression in UV‐irradiated human dermal fibroblasts

{\beta}

Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete

-glucosidase의 활성은 배양 96시간을 기준으로 최고 91.5 mU/mL로 확인되었으며, 감초추출발효물로부터 생성된 리퀴리티게닌과 이소리퀴리티게닌의 함량은 HPLC 분석을 통하여 최대

Preparation and characterization of quercetin-loaded silica microspheres stabilized by combined multiple emulsion and sol-gel processes

568.5{\mu}g/mL