Junki Lee

Limits on WIMP-nucleon cross section with CsI(Tl) crystal detectors

The Korea Invisible Mass Search(KIMS) experiment presents new limits on WIMP-nucleon cross section using the data from an exposure of 3409 kgd taken with low background CsI(Tl) crystals at Yangyang underground laboratory. The most stringent limit on the spin dependent interaction for pure proton case is obtained. The DAMA signal region for both spin independent and spin dependent interactions for the WIMP mass higher than 20 GeV/c^2are excluded by the single experiment with crystal scintillators.

New Limits on Interactions between Weakly Interacting Massive Particles and Nucleons Obtained with CsI(Tl) Crystal Detectors

The Korea Invisible Mass Search (KIMS) experiment presents new limits on the weakly interacting massive particle (WIMP)-nucleon cross section using data from an exposure of 3409 kg.d taken with low-background CsI(Tl) crystals at the Yangyang Underground Laboratory. The most stringent limit on the spin-dependent interaction for a pure proton case is obtained. The DAMA signal region for both spin-independent and spin-dependent interactions for the WIMP masses greater than 20 GeV/c2 is excluded by the single experiment with crystal scintillators.

Comprehensive Survey of Genetic Diversity in Chloroplast Genomes and 45S nrDNAs within Panax ginseng Species

We report complete sequences of chloroplast (cp) genome and 45S nuclear ribosomal DNA (45S nrDNA) for 11 Panax ginseng cultivars. We have obtained complete sequences of cp and 45S nrDNA, the representative barcoding target sequences for cytoplasm and nuclear genome, respectively, based on low coverage NGS sequence of each cultivar. The cp genomes sizes ranged from 156,241 to 156,425 bp and the major size variation was derived from differences in copy number of tandem repeats in the ycf1 gene and in the intergenic regions of rps16-trnUUG and rpl32-trnUAG. The complete 45S nrDNA unit sequences were 11,091 bp, representing a consensus single transcriptional unit with an intergenic spacer region. Comparative analysis of these sequences as well as those previously reported for three Chinese accessions identified very rare but unique polymorphism in the cp genome within P. ginseng cultivars. There were 12 intra-species polymorphisms (six SNPs and six InDels) among 14 cultivars. We also identified five SNPs from 45S nrDNA of 11 Korean ginseng cultivars. From the 17 unique informative polymorphic sites, we developed six reliable markers for analysis of ginseng diversity and cultivar authentication.

Real-Time Tool Breakage Monitoring for NC Milling Process

Abstract This paper presents real-time tool breakage detection in NC milling process by monitoring cutting force. Cutting force was indirectly measured by the feed drive AC motor current. Modeling of the feed drive system and calibration with a tool dynamometer show that the sensitivity of the feed drive motor current is sufficient to characterize tool breakage. AR 1 model with tool-runout is used to determine tool breakage. The algorithm was implemented on a Digital Signal Processing (DSP) board for real-time tool breakage monitoring. Experimental works showed an excellent real-time monitoring capability of the proposed tool breakage detection system.

Complete chloroplast and ribosomal sequences for 30 accessions elucidate evolution of Oryza AA genome species.

Cytoplasmic chloroplast (cp) genomes and nuclear ribosomal DNA (nR) are the primary sequences used to understand plant diversity and evolution. We introduce a high-throughput method to simultaneously obtain complete cp and nR sequences using Illumina platform whole-genome sequence. We applied the method to 30 rice specimens belonging to nine Oryza species. Concurrent phylogenomic analysis using cp and nR of several of specimens of the same Oryza AA genome species provides insight into the evolution and domestication of cultivated rice, clarifying three ambiguous but important issues in the evolution of wild Oryza species. First, cp-based trees clearly classify each lineage but can be biased by inter-subspecies cross-hybridization events during speciation. Second, O. glumaepatula, a South American wild rice, includes two cytoplasm types, one of which is derived from a recent interspecies hybridization with O. longistminata. Third, the Australian O. rufipogan-type rice is a perennial form of O. meridionalis.

CaMoO/sub 4/ scintillation crystal for the search of /sup 100/Mo double beta decay

Scintillation properties of calcium molybdate are investigated. The emission spectrum is broad with a maximum at 520 nm. At room temperature the light yield is 4% of CsI(Tl) (400 photoelectrons/MeV) and the decay time is 17 /spl mu/s. During cooling the light yield and decay time increase. Prospects of optimization of this scintillator are also discussed. The feasibility of the material for the search of neutrinoless double beta decay of /sup 100/Mo is analyzed. A half life limit of T/sub 1/2/=1/spl middot/10/sup 25/ years (68% CL) seems feasible with CaMoO/sub 4/ scintillator, running with 10 kg of /sup 100/Mo for 5 years. The background due to two neutrino double beta decay of /sup 48/Ca is also considered. Requirements for new scintillators for double beta decay search are formulated.

Feed-Rate Optimization of Ball End Milling Considering Local Shape Features

Abstract In ball end milling of free form surfaces, static and dynamic cutting characteristics change dramatically for different local shape features. Local shape features are classified as plain, upward ramp, downward ramp, contour, convex corner and concave corner. Data base for stable cutting conditions of zinc base alloy was established through various experiments with different local shape features. Feed rate optimization program, Opticode, was developed from the relationship between optimal feed rate and local shape feature. In Opticode, local features are recognized by comparing NC codes of neighboring points. The developed program was applied to automobile prototype production dies.

Exceptional reduction of the plastid genome of saguaro cactus (Carnegiea gigantea): Loss of the ndh gene suite and inverted repeat

UNLABELLED • PREMISE OF THE STUDY Land-plant plastid genomes have only rarely undergone significant changes in gene content and order. Thus, discovery of additional examples adds power to tests for causes of such genome-scale structural changes.• METHODS Using next-generation sequence data, we assembled the plastid genome of saguaro cactus and probed the nuclear genome for transferred plastid genes and functionally related nuclear genes. We combined these results with available data across Cactaceae and seed plants more broadly to infer the history of gene loss and to assess the strength of phylogenetic association between gene loss and loss of the inverted repeat (IR).• KEY RESULTS The saguaro plastid genome is the smallest known for an obligately photosynthetic angiosperm (∼113 kb), having lost the IR and plastid ndh genes. This loss supports a statistically strong association across seed plants between the loss of ndh genes and the loss of the IR. Many nonplastid copies of plastid ndh genes were found in the nuclear genome, but none had intact reading frames; nor did three related nuclear-encoded subunits. However, nuclear pgr5, which functions in a partially redundant pathway, was intact.• CONCLUSIONS The existence of an alternative pathway redundant with the function of the plastid NADH dehydrogenase-like complex (NDH) complex may permit loss of the plastid ndh gene suite in photoautotrophs like saguaro. Loss of these genes may be a recurring mechanism for overall plastid genome size reduction, especially in combination with loss of the IR.

A New Parallel Mechanism Machine Tool Capable of Five-Face Machining

Abstract This paper introduces a new parallel mechanism machine tool that can machine the top surface and four lateral surfaces of a prismatic workpiece with one setup. No B-axis motion is necessary. Five-axis simultaneous machining can be executed in any workspace. This machine is based on a new parallel mechanism, Eclipse , which was invented by the authors. The Eclipse allows the spindle of the machine tool to tilt to 90 degrees from the vertical posture, and also to sweep the lateral surfaces of the workpiece over 360 degrees. This paper presents the overall structure, the detailed design aspects, and the performance evaluation of the new parallel mechanism machine tool.

New reference genome sequences of hot pepper reveal the massive evolution of plant disease-resistance genes by retroduplication

BackgroundTransposable elements are major evolutionary forces which can cause new genome structure and species diversification. The role of transposable elements in the expansion of nucleotide-binding and leucine-rich-repeat proteins (NLRs), the major disease-resistance gene families, has been unexplored in plants.ResultsWe report two high-quality de novo genomes (Capsicum baccatum and C. chinense) and an improved reference genome (C. annuum) for peppers. Dynamic genome rearrangements involving translocations among chromosomes 3, 5, and 9 were detected in comparison between C. baccatum and the two other peppers. The amplification of athila LTR-retrotransposons, members of the gypsy superfamily, led to genome expansion in C. baccatum. In-depth genome-wide comparison of genes and repeats unveiled that the copy numbers of NLRs were greatly increased by LTR-retrotransposon-mediated retroduplication. Moreover, retroduplicated NLRs are abundant across the angiosperms and, in most cases, are lineage-specific.ConclusionsOur study reveals that retroduplication has played key roles for the massive emergence of NLR genes including functional disease-resistance genes in pepper plants.