Jyh-Jong Chang

(-)-Epigallocatechin-3-gallate improves bone microarchitecture in ovariectomized rats

ObjectivePreviously, we reported that (−)-epigallocatechin-3-gallate (EGCG), a green tea polyphenol, increased the osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the messenger RNA expression of osteogenesis-related genes, alkaline phosphatase activity, and, eventually, mineralization. The present study further investigated the effects of EGCG on bone microstructure change and possible mechanisms in ovariectomy (OVX)–induced osteopenic rats. MethodsRats subjected to OVX were administered EGCG systemically for 12 weeks. Proximal tibial bone mineral densities before and after treatment were compared between groups. Changes in the microarchitecture of both the proximal tibia and the third lumbar spine were compared between EGCG-treated and nontreated groups using micro-CT (&mgr;CT). Bone histology and immunohistochemistry in the proximal tibia were evaluated. ResultsResults showed that EGCG 3.4 mg/kg/day (estimated peak serum concentration, 10 &mgr;mol/L) hampered the decrease in bone mineral density (from 7.97% to 3.96%) and improved the parameters of &mgr;CT measurements, including bone volume (from 18% to 27%), trabecular thickness (from 0.17 to 0.22 mm), trabecular number (from 1.13 to 1.37 mm−1), and trabecular separation (from 0.91 to 0.69 mm), compared with nontreated ovariectomized rats. Similar improvements in bone volume (from 30% to 49%) and trabecular thickness (from 0.14 to 0.26 mm) were also found in the third lumbar spine. Bone volume in the tibial cortex also increased after EGCG treatment (from 9% to 28%). A higher trabecular number and greater trabecular volume were also seen in histology, further confirming the results of &mgr;CT. The immunolocalized bone morphogenetic protein 2 brown-stained area increased from 31% in the OVX group to 53% in the OVX + 10 EGCG group (P < 0.01). Serial biochemistry data revealed no significant systemic toxic effect of EGCG. ConclusionsIntraperitoneal treatment with EGCG 3.4 mg/kg/day for 3 months can mitigate bone loss and improve bone microarchitecture in ovariectomized rats, and increased expression of bone morphogenetic protein 2 may contribute to this effect.

Effect of bilateral reaching on affected arm motor control in stroke – with and without loading on unaffected arm

Purpose. To investigate the effect of bilateral reaching, with/without inertial loading on the unaffected arm, on hemiparetic arm motor control in stroke. Methods. Twenty unilateral stroke patients were recruited. A three-dimensional optical motion capture system was used to measure the movement trajectory of the hemiparetic arm while performing three tasks: affected limb reaching forward; two-limb reaching forward; and two-limb reaching forward with inertia loading of 25% upper limb weight on the unaffected limb, respectively. Kinematical parameters were utilized to quantify the reaching performance of the affected arm. Results. No matter whether loading was applied on the unaffected arm or not, the bilateral reaching task did not significantly facilitate smoother and faster movement. Furthermore, during bilateral reaching task with/without loading on the unaffected arm, stroke patients showed slower movement, lower maximal movement velocity, feedback control dominant and discontinuous movements in the affected arm than the same task with unilateral reaching. Subjects showed the greatest active upper extremity range of motion in proximal joints during the bilateral reaching task without unaffected arm loading. The amount of trunk movement also increased during bilateral reaching either with or without loading on the unaffected arm. Patients with moderate upper extremity motor impairment performed more discontinuous movements and less active elbow range of motion during bilateral reaching tasks; however, those with mild upper extremity motor impairment performed smoother movements and demonstrated greater active elbow range of motion during bilateral reaching tasks. Conclusions. Bilateral reaching tasks with/without loading on the unaffected arm could be considered as adding challenges during motor control training. Training with bilateral arm movements may be considered as a treatment strategy, and can be incorporated in stroke rehabilitation to facilitate greater arm active movement and improve motor control performance in the affected arm.

EFFECTS OF SPEED AND INCLINE ON LOWER EXTREMITY KINEMATICS DURING TREADMILL JOGGING IN HEALTHY SUBJECTS

Recently, there are more people jogging with a treadmill at the gym or the home setting. The main available selected modes for treadmill jogging are speed and slope of incline. Increased speeds and incline slopes will not only increase the cardiopulmonary loading but may also alter the lower extremity (LE) movement patterns. There are few systematic investigations of the effect of the speed and incline on LE kinematics. Most studies have used 2D methods which focused on movements in sagittal plane only and this has limitations in the acquired data since lower extremity movements also include frontal and transverse planes. The current study aimed to investigate LE movement during jogging at different speeds and incline slopes using a high speed three-dimensional (3D) motion analysis system. Eighteen young healthy males were recruited. The video-based motion capture system with six CCD cameras, HIRES Expert Vision System (Motion Analysis Corporation, CA, USA), was used to collect kinematic data at a sampling frequency of 120Hz. Nineteen passive reflective markers were attached to bilateral lower extremities of the subject. The joint angle is calculated by Euler angle using the rotation sequence: 2-1-3 (y-x′-z″). Four speeds were selected: 2 m/s, 2.5 m/s, 3 m/s, 3.5 m/s with the slope at 0, and four slopes were selected: 0%, 5%,10%,15% at a speed of 3 m/s. Repeated-measures ANOVA was used to test hypotheses regarding changes in jogging condition on LE kinematic variables. The significance level was set at 0.05. As the jogging slope increased, the hip, knee and ankle demonstrated a significantly greater maximum flexion in swing phase (p<0.001), but the maximum extension angles in stance phase were relatively unchanged. Increased LE flexion during swing phase is important to ensure foot clearance with increased slope. For increased speed, the hip and ankle joints had significantly greater maximum joint extension angles during stance phase and the hip and knee joint had significantly larger maximum flexion angles in swing phase (p<0.001). Increased motion during swing phase account for a larger step length and increased motion during stance phase may facilitate the generation of power during forward propulsion as the jogging speed increased. As the slope and speed increased, LE movement patterns were changed in the transverse plane: the significantly increased (p<0.01) internal hip rotation at terminal stance, the increased toe-in of foot (p<0.001) during terminal stance phase and decreased (p<0.05) toe-out during swing phase. Increased hip motion in transverse plane could lengthen the stride distance and increase foot toe-in for providing a stable lever for push off to increase propulsion force as speed or slope is increased. By way of systematic 3D kinematic investigation of the LE in jogging, the results further elucidate the effect of changing speed and incline on LE joints movements. This information could provide guidelines for rehabilitation clinicians or coaches to select an appropriate training mode for jogging.

EMG AND PLANTAR PRESSURE PATTERNS AFTER PROLONGED RUNNING

The aim of this paper is to investigate the effect of prolonged running on lower limb muscle activity, foot pressure and foot contact area. The treadmill running test was performed at a running velocity of 12 km/h for 20 minutes. Twenty-nine male students from the Army Infantry School took part in this study. For all subjects in our study, a number of variables were analyzed by the prolonged running. The EMG variables included the signal maximum amplitude of EMG linear envelope of all the muscles. Meanwhile, maximal forces and peak foot pressures in 10 anatomically defined areas of the foot, and contact area of the whole foot were analyzed. Running EMG data in each of the phases (phase 2–4) were compared to those at the beginning of the run (phase 1). Dynamic pedography data in phase 4 was compared to those of phase 1. Pedography analysis revealed a significant increase in the maximal forces and peak pressures under the medial midfoot and all forefoot regions. From phase 1 to phase 4, the maximal force increased by 32% under the medial midfoot, 29% under the first metatarsal, 34% under the second and third metatarsal, and 21% under the fourth and fifth metatarsal. The peak pressure under the medial midfoot increased by 19%, under the first metatarsal increased by 21%, under the second and third metatarsal increased by 31%, and under the fourth and fifth metatarsal increased by 21%. The averaged maximum EMG amplitudes of almost all the muscles were increased gradually as time increased. Among them, rectus femoris, gastrocnemius, soleus, and tibialis anterior muscles reach a significant amplitude at the p < 0.05 level. In conclusion, our results showed that a prolonged running under a 20 minutes limitation led to a greater increase in muscle amplitude, midfoot and forefoot loading compared with the beginning of running.

KINEMATIC WRIST AND THUMB POSTURE ANALYSIS OF SCHOOL CHILDREN DURING THE MANIPULATION OF THE MOUSE

The wrist and thumb postures adopted during the use of three different types of computer mouse (mini mouse; standard mouse; MouseMan) to perform eight standardized tasks were evaluated. Thirty elementary students between seven to twelve years of age were studied using a magnetic tracking system. The peak value and angular range of motion of the wrist and thumb extension/flexion and radial/ulnar deviation were measured during performing 8 standard tasks. There were statistically significant larger values in the standard mice with regard to extreme wrist extension. Angular range of wrist extension observed for the standard mice were also much higher than the mini mouse and MouseMan. There were statistically significant larger values in the MouseMan with regard to extreme wrist ulnar deviation, and an average decrease in extreme thumb flexion and angular range of thumb flexion. The results from the questionnaire showed that most elementary students preferred to use the standard mouse (40%) next was the MouseMan (37%); and the last the mini mouse (23%). The recommendation of mouse types based on the hand length for elementary students was not statistically significant in our study.