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Dive into the research topics where K.R. O'Neill is active.

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Featured researches published by K.R. O'Neill.


Journal of Immunology | 2003

A Causative Relationship Exists Between Eosinophils and the Development of Allergic Pulmonary Pathologies in the Mouse

H.H. Shen; Sergei I. Ochkur; Michael P. McGarry; Jeffrey R. Crosby; Edie M. Hines; Michael T. Borchers; Huiying Wang; Travis L. Biechelle; K.R. O'Neill; Tracy Ansay; Dana Colbert; Stephania A. Cormier; J. Paul Justice; Nancy A. Lee; James J. Lee

Asthma and mouse models of allergic respiratory inflammation are invariably associated with a pulmonary eosinophilia; however, this association has remained correlative. In this report, a causative relationship between eosinophils and allergen-provoked pathologies was established using eosinophil adoptive transfer. Eosinophils were transferred directly into the lungs of either naive or OVA-treated IL-5−/− mice. This strategy resulted in a pulmonary eosinophilia equivalent to that observed in OVA-treated wild-type animals. A concomitant consequence of this eosinophil transfer was an increase in Th2 bronchoalveolar lavage cytokine levels and the restoration of intracellular epithelial mucus in OVA-treated IL-5−/− mice equivalent to OVA-treated wild-type levels. Moreover, the transfer also resulted in the development of airway hyperresponsiveness. These pulmonary changes did not occur when eosinophils were transferred into naive IL-5−/− mice, eliminating nonspecific consequences of the eosinophil transfer as a possible explanation. Significantly, administration of OVA-treated IL-5−/− mice with GK1.5 (anti-CD4) Abs abolished the increases in mucus accumulation and airway hyperresponsiveness following adoptive transfer of eosinophils. Thus, CD4+ T cell-mediated inflammatory signals as well as signals derived from eosinophils are each necessary, yet alone insufficient, for the development of allergic pulmonary pathology. These data support an expanded view of T cell and eosinophil activities and suggest that eosinophil effector functions impinge directly on lung function.


Journal of Immunology | 2007

Coexpression of IL-5 and Eotaxin-2 in Mice Creates an Eosinophil-Dependent Model of Respiratory Inflammation with Characteristics of Severe Asthma

Sergei I. Ochkur; Elizabeth A. Jacobsen; Cheryl A. Protheroe; Travis L. Biechele; R.S. Pero; Michael P. McGarry; Huiying Wang; K.R. O'Neill; Dana Colbert; Thomas V. Colby; Huahao Shen; Michael R. Blackburn; Charles C. Irvin; James J. Lee; Nancy A. Lee

Mouse models of allergen provocation and/or transgenic gene expression have provided significant insights regarding the cellular, molecular, and immune responses linked to the pathologies occurring as a result of allergic respiratory inflammation. Nonetheless, the inability to replicate the eosinophil activities occurring in patients with asthma has limited their usefulness to understand the larger role(s) of eosinophils in disease pathologies. These limitations have led us to develop an allergen-naive double transgenic mouse model that expresses IL-5 systemically from mature T cells and eotaxin-2 locally from lung epithelial cells. We show that these mice develop several pulmonary pathologies representative of severe asthma, including structural remodeling events such as epithelial desquamation and mucus hypersecretion leading to airway obstruction, subepithelial fibrosis, airway smooth muscle hyperplasia, and pathophysiological changes exemplified by exacerbated methacholine-induced airway hyperresponsiveness. More importantly, and similar to human patients, the pulmonary pathologies observed are accompanied by extensive eosinophil degranulation. Genetic ablation of all eosinophils from this double transgenic model abolished the induced pulmonary pathologies, demonstrating that these pathologies are a consequence of one or more eosinophil effector functions.


Journal of Leukocyte Biology | 2006

Pivotal Advance : Eosinophil infiltration of solid tumors is an early and persistent inflammatory host response

Stephania A. Cormier; Anna G. Taranova; Carrie E. Bedient; Thanh Nguyen; Cheryl A. Protheroe; R.S. Pero; Dawn Dimina; Sergei I. Ochkur; K.R. O'Neill; Dana Colbert; Theresa R. Lombari; Stephanie L. Constant; Michael P. McGarry; James J. Lee; Nancy A. Lee

Tumor‐associated eosinophilia has been observed in numerous human cancers and several tumor models in animals; however, the details surrounding this eosinophilia remain largely undefined and anecdotal. We used a B16‐F10 melanoma cell injection model to demonstrate that eosinophil infiltration of tumors occurred from the earliest palpable stages with significant accumulations only in the necrotic and capsule regions. Furthermore, the presence of diffuse extracellular matrix staining for eosinophil major basic protein was restricted to the necrotic areas of tumors, indicating that eosinophil degranulation was limited to this region. Antibody‐mediated depletion of CD4+ T cells and adoptive transfer of eosinophils suggested, respectively, that the accumulation of eosinophils is not associated with T helper cell type 2‐dependent immune responses and that recruitment is a dynamic, ongoing process, occurring throughout tumor growth. Ex vivo migration studies have identified what appears to be a novel chemotactic factor(s) released by stressed/dying melanoma cells, suggesting that the accumulation of eosinophils in tumors occurs, in part, through a unique mechanism dependent on a signal(s) released from areas of necrosis. Collectively, these studies demonstrate that the infiltration of tumors by eosinophils is an early and persistent response that is spatial‐restricted. It is more important that these data also show that the mechanism(s) that elicit this host response occur, independent of immune surveillance, suggesting that eosinophils are part of an early inflammatory reaction at the site of tumorigenesis.


Proceedings of the National Academy of Sciences of the United States of America | 2007

Gαi2-mediated signaling events in the endothelium are involved in controlling leukocyte extravasation

R.S. Pero; Michael T. Borchers; Karsten Spicher; Sergei I. Ochkur; Lyudmila Sikora; Savita P. Rao; Hiam Abdala-Valencia; K.R. O'Neill; Huahao Shen; Michael P. McGarry; Nancy A. Lee; Joan M. Cook-Mills; P. Sriramarao; Melvin I. Simon; Lutz Birnbaumer; James J. Lee

The trafficking of leukocytes from the blood to sites of inflammation is the cumulative result of receptor-ligand-mediated signaling events associated with the leukocytes themselves as well as with the underlying vascular endothelium. Our data show that Gαi signaling pathways in the vascular endothelium regulate a critical step required for leukocyte diapedesis. In vivo studies using knockout mice demonstrated that a signaling event in a non-lymphohematopoietic compartment of the lung prevented the recruitment of proinflammatory leukocytes. Intravital microscopy showed that blockade was at the capillary endothelial surface andex vivo studies of leukocyte trafficking demonstrated that a Gαi-signaling event in endothelial cells was required for transmigration. Collectively, these data suggest that specific Gαi2-mediated signaling between endothelial cells and leukocytes is required for the extravasation of leukocytes and for tissue-specific accumulation.


The Journal of Allergy and Clinical Immunology | 2003

A causative relationship exists between eosinophils and the development of allergic pulmonary pathologies

Nancy A. Lee; H.H. Shen; Jeffrey R. Crosby; Edith M. Hines; Michael T. Borchers; Michael P. McGarry; Sergei I. Ochkur; T.L. Biechele; K.R. O'Neill; Tracy Ansay; Dana Colbert; Stephania A. Cormier; Joshua Paul Justice; James J. Lee

Asthma and mouse models of allergic respiratory inflammation are invariably associated with a pulmonary eosinophilia; however, this association has remained correlative. In this report, a causative relationship between eosinophils and allergen-provoked pathologies was established using eosinophil adoptive transfer. Eosinophils were transferred directly into the lungs of either naive or OVA-treated IL-5(-/-) mice. This strategy resulted in a pulmonary eosinophilia equivalent to that observed in OVA-treated wild-type animals. A concomitant consequence of this eosinophil transfer was an increase in Th2 bronchoalveolar lavage cytokine levels and the restoration of intracellular epithelial mucus in OVA-treated IL-5(-/-) mice equivalent to OVA-treated wild-type levels. Moreover, the transfer also resulted in the development of airway hyperresponsiveness. These pulmonary changes did not occur when eosinophils were transferred into naive IL-5(-/-) mice, eliminating nonspecific consequences of the eosinophil transfer as a possible explanation. Significantly, administration of OVA-treated IL-5(-/-) mice with GK1.5 (anti-CD4) Abs abolished the increases in mucus accumulation and airway hyperresponsiveness following adoptive transfer of eosinophils. Thus, CD4(+) T cell-mediated inflammatory signals as well as signals derived from eosinophils are each necessary, yet alone insufficient, for the development of allergic pulmonary pathology. These data support an expanded view of T cell and eosinophil activities and suggest that eosinophil effector functions impinge directly on lung function.


Science | 2004

Defining a Link with Asthma in Mice Congenitally Deficient in Eosinophils

James J. Lee; Dawn Dimina; Mi Mi P. Macias; Sergei I. Ochkur; Michael P. McGarry; K.R. O'Neill; Cheryl A. Protheroe; R.S. Pero; Thanh Nguyen; Stephania A. Cormier; Elizabeth Lenkiewicz; Dana Colbert; Lisa Rinaldi; Steven J. Ackerman; Charles G. Irvin; Nancy A. Lee


Contemporary Topics in Laboratory Animal Science | 2005

Decreased size and survival of weanling mice in litters of IL-5-/ -mice are a consequence of the IL-5 deficiency in nursing dams.

Dana Colbert; Michael P. McGarry; K.R. O'Neill; Nancy A. Lee; James J. Lee


The Journal of Allergy and Clinical Immunology | 2008

Is Eosinophil Dependence of Pulmonary Allergic Inflammation in Mice Really Strain Specific

Sergei I. Ochkur; Elizabeth A. Jacobsen; Cheryl A. Protheroe; R.S. Pero; K.R. O'Neill; Anna G. Taranova; Dana Colbert; Alfred D. Doyle; Charles G. Irvin; Nancy A. Lee; James J. Lee


The Journal of Allergy and Clinical Immunology | 2007

Extravasation of Leukocytes from Circulation is Controlled by Gαi2 Signaling Events in the Endothelium

R.S. Pero; Michael T. Borchers; Karsten Spicher; Sergei I. Ochkur; L. Sikora; Savita P. Rao; K.R. O'Neill; Hiam Abdala-Valencia; H.H. Shen; Melvin I. Simon; Michael P. McGarry; Nancy A. Lee; Joan M. Cook-Mills; P. Sriramarao; Lutz Birnbaumer; James J. Lee


The Journal of Allergy and Clinical Immunology | 2007

Co-Expression of IL-5 and Eotaxin-2 in Mice Creates a Novel and Eosinophil-dependent Pulmonary Model of Respiratory Inflammation Characteristic of Human Disease

Sergei I. Ochkur; T. Biechele; Elizabeth A. Jacobsen; B. McElhinney; Cheryl A. Protheroe; R.S. Pero; Michael T. Borchers; K.R. O'Neill; Dana Colbert; Michael P. McGarry; Yvonne M. W. Janssen-Heininger; Charles G. Irvin; H.H. Shen; James J. Lee; Nancy A. Lee

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Michael T. Borchers

University of Cincinnati Academic Health Center

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