K.R. Page
University of Aberdeen
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Featured researches published by K.R. Page.
Placenta | 1997
P. Haggarty; K.R. Page; D.R. Abramovich; J. Ashton; D Brown
The role of the placenta in controlling the supply of fatty acids to the fetus was investigated in term placentae (n = 9) from normal pregnancies. The maternal side was perfused ex vivo for 90 min with a modified Krebs Ringer solution containing a physiological mixture of fatty acids and ratio of fatty acid to human albumin. There was no evidence of chain elongation and desaturation of the essential fatty acids. Relative to the value for oleic acid, the rate of transfer to the fetal circulation was: 1.30 +/- 0.02 (P < 0.001) for linoleic acid, 1.61 +/- 0.09 (P = 0.002) for alpha-linolenic acid, 0.67 +/- 0.10 (P = 0.033) for arachidonic acid and 2.10 +/- 0.16 (P = 0.003) for docosahexaenoic acid. For tissue accumulation the values were 1.47 +/- 0.39 (P < 0.001) for linoleic acid, 2.24 +/- 0.37 (P = 0.027) for alpha-linolenic acid, 9.84 +/- 1.03 (P = 0.001) for arachidonic acid, and 3.01 +/- 0.79 (P = 0.064) for docosahexaenoic acid. The order of selectivity for transfer from the maternal to the fetal circulation was docosahexaenoic > alpha-linolenic > linoleic > oleic > arachidonic acid. Such a mechanism would allow the preferential transfer of docosahexaenoic acid and the essential fatty acids to the fetal circulation, thereby protecting the polyunsaturated fatty acid supply to the fetus during a critical period of development.
Neonatology | 1999
P. Haggarty; J. Ashton; M. Joynson; D.R. Abramovich; K.R. Page
The role of the placenta in controlling the supply of fatty acids to the fetus was investigated in term placentas (n = 5) from normal pregnancies. The maternal side was perfused ex vivo for 90 min with a modified Krebs Ringer solution containing a physiological mixture of fatty acids – designed to mimic the composition of non-esterified fatty acids (NEFA) measured in the last trimester of pregnancy (n = 10) – and ratio of fatty acid to human albumin. The selectivity for α-linolenic acid (αLN) transfer to the fetal circulation was not significantly different from that observed when using the triglyceride (TG) composition (1.21 ± 0.04), but significantly different for AA (1.43 ± 0.12; p < 0.001) and docosahexaenoic acid (DHA; 2.02 ± 0.09; p = 0.048). The absolute rate of transfer (nmol · ml–1) compared to that using the TG maternal perfusate composition was significantly different for LA (0.562 ± 0.038; p = 0.50), αLN (0.130 ± 0.009; p < 0.001), arachidonic acid (AA; 0.218 ± 0.022; p = 0.001) and DHA (0.383 ± 0.04; p < 0.001). Thus, placental selectivity for αLN and DHA appears to be relatively unresponsive to changes in the mixture of fatty acids in the maternal circulation but the selectivity for AA increased with the increase in the maternal AA concentration. For an 8-fold increase in the concentration of DHA in the maternal circulation there was a 13-fold increase in the transfer of DHA to the fetal circulation. For a 2-fold increase in the concentration of AA, transfer was increased 8-fold. For a 1.3-fold increase in the concentration of αLN, transfer was increased 2.1-fold. These results suggest that the maternal concentration of individual fatty acids, and hence the composition of the maternal diet, can have large effects on polyunsaturated/long-chain polyunsaturated fatty acids delivery to the fetus.
Placenta | 1992
K.R. Page; D.R. Abramovich; P.J. Aggett; M. Bain; A.R. Chipperfield; H. Durdy; J. McLachlan; A. Smale
The uptake of Zinc (Zn) by microvillus border membrane vesicles formed from the trophoblast of term human placentae is markedly increased over brief periods of incubation with much slower increases persisting for up to 2 h of incubation. Zinc is both bound to membrane components and transported into intravesicular osmotically active space. Uptake is saturable, temperature dependent from 4 to 37 degrees C with a Q10 of 1.7, and is inhibited by the sulphydryl agent DTNB. About 20 per cent of the uptake is susceptible to inhibition by Cadmium (Cd) at concentrations from 5 to 50 microM, a significant part of the action of this metal being on the transmembrane component of Zn uptake.
Calcified Tissue International | 1991
J. M. A. Williams; D.R. Abramovich; C. G. Dacke; T. M. Mayhew; K.R. Page
SummaryHuman term placental lobules were dually perfused with Krebs Ringer solution at 37°C under open circuit conditions. Provided that perfusate Ca2+ concentrations were between 2.33 and 2.55 mM, there was a steady release of Ca2+ into the fetal circulation and uptake of Ca2+ from the maternal circulation. There was no significant calcium (Ca) protein binding in the perfusates. Addition of dinitrophenol altered the release of Ca2+ to an uptake on the fetal circuit and enhanced Ca2+ uptake on the maternal circuit. It also produced a release of potassium (K)+ and an uptake of Na+ on both sides of the placenta. Ouabain had no significant effect on Ca movements although it produced a marked release of K+ into the fetal perfusate. The effect of cooling on the fetal circuit was similar to that of dinitrophenol (DNP), although it did not produce significant changes in either Ca2+ or K+ movements on the maternal side of the lobule. Both DNP and cooling reduced the Ca concentration ratio between fetal and maternal outflows to unity. Replacement of Na+ by choline Ringer had only transient effect on the extraction of45Ca from fetal perfusate. These observations indicate that a Ca2+/Na (sodium)+ exchanger does not make a major contribution to the transplacental movement of Ca2+ from mother to fetus and that this process is more probably associated with membrane-bound ATPases.
Journal of Membrane Science | 2002
K.R. Page; Peter G. Bush; D.R. Abramovich; M Aggett; M.D Burke; Terry M. Mayhew
Ingested tobacco smoke contains many toxic components that may harm the membrane compartments of the human placenta. The effects of maternal smoking on placental membrane structure are examined by stereological methods and related to smoking habit. A significant decrease in fetal capillary volume and increase in the diffusion distance across the trophoblastic epithelium is observed. The stereologically-determined structural variables are used to estimate both total and partial oxygen diffusive conductances. The total diffusive conductance is found not to alter with smoking; however, changes in partial conductances and the haematocrits of both maternal and fetal blood, indicate hypoxic stress is associated with smoking. However, a component of the placental membranes responsible for the transport of alanine is shown to be altered. An increase in the sodium-dependent transport of alanine across the microvillous border membrane of the placenta is observed in tissues derived from mothers who smoke. This may be an adaptive response to a deficient supply of this amino acid to the placenta.
Archive | 1989
K.R. Page; D.R. Abramovich; C. G. Dacke; K. Henderson; A. Klopper
Alone of the in vitro methods of studying placental function, the dually-perfused placenta preserves the integrity of the fetal and maternal circulations so allowing studies of the “sidedness” of the organ, a point of particular relevance to feto-maternal endocrine physiology. Difficulties in studying the whole human placenta arise from the problems of obtaining a complete, uninjured specimen, and in maintaining adequate perfusion of the entire intervillous space. These difficulties may be minimised by perfusing a limited region of the placenta only, typically a lobule of 25 g wet weight.1 Lobules selected for this purpose are each supplied by a single fetal artery and vein which may be cannulated from the fetal surface. The maternal aspect is perfused by glass cannulae inserted into the lobular intervillous space, the outflow being collected by drainage from the maternal plate. The perfusate is a physiologically balanced solution such as Earle’s culture medium, Medium 199, or Krebs Ringer containing either albumin or Dextran. Perfusions can be maintained for three to 12 hours.
Journal of Membrane Science | 2002
K.R. Page; Peter G. Bush; D.R. Abramovich; P.J. Aggett; M.D Burke; Terry M. Mayhew
K.R. Page a,∗, P. Bush b, D.R. Abramovich c, P.J. Aggett d, M.D. Burke e, T.M. Mayhew f a Department of Biomedical Sciences, University of Aberdeen, Aberdeen, Scotland, UK b Department of Biomedical Sciences (Physiology), University of Edinburgh, Edinburgh, Scotland, UK c Department of Obstetrics and Gynaecology, Aberdeen University, Aberdeen, UK d Lancashire Postgraduate School of Medicine and Health, University of Central Lancashire, Preston, UK e School of Pharmacy and Pharmaceutical Sciences, De Montfort University, Leicester, UK f School of Biomedical Sciences, Queens Medical Centre, Nottingham University, Nottingham, UK
Placenta | 2000
Peter G. Bush; Terry M. Mayhew; D.R. Abramovich; P.J. Aggett; M.D Burke; K.R. Page
Placenta | 2000
Peter G. Bush; Terry M. Mayhew; D.R. Abramovich; P.J. Aggett; M.D. Burke; K.R. Page
Placenta | 2000
Ruth Danzeisen; Sreenivasan Ponnambalam; Richard G. Lea; K.R. Page; Lorraine Gambling; Harry J. McArdle