Kandhasamy Sowndhararajan

Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L.

This study was designed to isolate and identify a potent inhibitory compound against nitric oxide (NO) production from the stem bark of Ulmus pumila L. Ethyl acetate fraction of hot water extract registered a higher level of total phenolics (756.93xa0mg GAE/g) and also showed strong DPPH (IC50 at 5.6xa0μg/mL) and ABTS (TEAC value 0.9703) radical scavenging activities than other fractions. Crude extract and its fractions significantly decreased nitrite accumulation in LPS-stimulated RAW 264.7 cells indicating that they potentially inhibited the NO production in a concentration dependent manner. Based on higher inhibitory activity, the ethyl acetate fraction was subjected to Sephadex LH-20 column chromatography and yielded seven fractions and all these fractions registered appreciable levels of inhibitory activity on NO production. The most effective fraction F1 was further purified and subjected to (1)H, (13)C-NMR and mass spectrometry analysis and the compound was identified as icariside E4. The results suggest that the U. pumila extract and the isolated compound icariside E4 effectively inhibited the NO production and may be useful in preventing inflammatory diseases mediated by excessive production of NO.

Antioxidant, inhibition of α-glucosidase and suppression of nitric oxide production in LPS-induced murine macrophages by different fractions of Actinidia arguta stem.

In traditional systems of medicine, fruits, leaves, and stems of Actinidia arguta (Sieb. et Zucc.) Planch. ex Miq. have been used to treat various inflammatory diseases. The present study determined the proximate composition, antioxidant, anti-inflammatory, and hypoglycemic potential of A. arguta stem. Phenolic composition of hot water extract and its sub-fractions was determined by Folin-Ciocalteus reagent method. In vitro antioxidant activities of the samples were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assays. Anti-inflammatory activity of different fractions was investigated through the inhibition of nitric oxide (NO) production in lipopolysaccharide (1xa0μg/ml) stimulated RAW 264.7 cells. In addition, inhibition of α-glucosidase activity of hot water extract was determined using p-nitrophenyl-α-d-glucopyranoside (pNPG) as a substrate. Ethyl acetate (557.23xa0mgxa0GAE/g) fraction contains higher level of total phenolic content. The antioxidant activity evaluated by DPPH radical scavenging assay showed a strong activity for ethyl acetate (IC50 of 14.28xa0μg/ml) and n-butanol fractions (IC50 of 48.27xa0μg/ml). Further, ethyl acetate fraction effectively inhibited NO production in RAW 264.7 cells induced by lipopolysaccharide (LPS) than other fractions (nitrite level to 32.14xa0μM at 200xa0μg/ml). In addition, hot water extract of A. arguta stem exhibited appreciable inhibitory activity against α-glucosidase enzyme with IC50 of 1.71xa0mg/ml. The obtained results have important consequence of using A. arguta stem toward the development of effective anti-inflammatory drugs.

Effect of essential oil and supercritical carbon dioxide extract from the root of Angelica gigas on human EEG activity

OBJECTIVESnThe present study aimed to investigate the effect of inhalation of essential oil (EO) and supercritical carbon dioxide extract (SC-CO2) from the root of A.xa0gigas on human electroencephalographic (EEG) activity.nnnMATERIALS AND METHODSnFor this purpose, the EO was obtained from the root of A.xa0gigas by steam distillation and SC-CO2 was obtained at 50xa0°C and 400xa0bar for 1xa0h. The EEG readings were recorded using the QEEG-8 system from 8 electrode sites according to the International 10-20 system.nnnRESULTSnIn the EEG study, the absolute low beta (left temporal and left parietal) activity significantly increased during the inhalation of EO. In the case of SC-CO2 inhalation, there was no significant change in absolute waves.nnnCONCLUSIONnThe results revealed that the EO of A.xa0gigas root produced significant changes in the absolute low beta activity and these changes may enhance the language learning abilities of human brain.

An overview of neuroprotective and cognitive enhancement properties of lignans from Schisandra chinensis

Schisandra chinensis fruits have been traditionally used for thousands of years in Korea, China and Japan to treat various ailments. The fruits contain a variety of bioactive metabolites, especially lignan components have been reported to have various biological activities and have potential in the treatment of numerous neurodegenerative diseases. The lignans from S. chinensis are mainly grouped under dibenzocyclooctadiene lignans. Previous studies have reported that the crude extracts and the isolated pure lignan components effectively protect the neuronal cell damage and significantly enhance the cognitive performances. The experimental findings support the extracts and lignan components from S. chinensis can be used as new therapeutic agents to treat various neurodegenerative diseases. In the current review, we highlight the lignans from S. chinensis as promising resources for the development of natural and effective agents for neuroprotective and cognitive enhancement effects. The lignan extracts and individual compounds from S. chinensis were summarized in relation to their neuroprotective and cognitive enhancement activities.

Baicalein as a potent neuroprotective agent: A review

In recent times, neurodegenerative diseases are the most challenging global health problems. Neuronal cell death or damage is a key factor for many neurodegenerative disorders. Therefore, there has been a growing interest in the development of effective neuroprotective agents, especially from natural sources. In particular, phytochemicals have shown high efficacy with low side effects in various in vitro and in vivo studies. In the various phytoconstituents, flavonoids are important bioactive products and mainly found in various vegetables and fruits. Among them, baicalein is one of the important flavones, which is mainly found in the root of Scutellaria baicalensis Georgi. A number of studies have reported that baicalein has potent neuroprotective properties under in vitro as well as in vivo systems. Hence, the purpose of this paper is to provide a review of the existing literature in connection with the neuroprotective effects of baicalein and its molecular mechanisms of action. The current review highlights could be useful to identify novel therapeutic agents in relation to the treatment of neurotoxicity-mediated diseases.

A role of Ficus species in the management of diabetes mellitus: A review

ETHNOPHARMACOLOGICAL RELEVANCEnDiabetes mellitus is one of the most common global health concerns, with a rapidly increasing incidence. A variety of medicinal plants, particularly those belonging to the genus Ficus (Moraceae), and their active compounds have been used to treat diabetes and related chronic disorders since ancient times.nnnAIM OF THE STUDYnThe aim of this review is to provide information regarding traditional and scientific knowledge of Ficus species with antidiabetic activity to researchers.nnnMATERIALS AND METHODSnA literature search was conducted to obtain information about the antidiabetic properties of Ficus from the electronic databases. Common and scientific names of various Ficus species were used as keywords for the search, along with the terms antidiabetic, hypoglycemic and diabetes.nnnRESULTSnAmong the assorted species of Ficus that were included in our search, F. benghalensis, F. carica, F. glomerata, F. glumosa, F. racemosa, and F. religiosa exhibited remarkable antidiabetic properties with various mechanisms of action. Moreover, Ficus species are versatile sources of bioactive metabolites such as flavonoids, phenolic acids, tannins, alkaloids, glycosides, coumarins, triterpenoids, sterols and vitamin E. These extracts and isolated compounds significantly have enhanced insulin secretion and subsequently reduced blood glucose level in various in vivo studies.nnnCONCLUSIONnThis review summarizes the antidiabetic potentials of the genus Ficus, including pharmacological studies with mechanisms of action as well as ethnobotanical uses. This review can help inform future scientific research towards the development of novel antidiabetic drugs.

Fragrant Chemicals in the Supercritical Carbon Dioxide Extract of Magnolia kobus DC. Flower Buds Increase the Concentration State of Brain Function

Abstract In traditional Korean medicine, flower buds of Magnolia kobus DC. plant is widely used for the treatments of various disorders. Hence, the present study was undertaken to evaluate the effect of supercritical carbon dioxide extract (SFE) of M. kobus flower buds on electroencephalographic (EEG) changes of human brain. The extract from the flower buds of M. kobus was obtained by SFE and the yield was 0.27% at 40°C and 400 bar pressure. Subsequently, GC-MS analysis revealed that the major components of the SFE were caryophyllene (19.93%) and α-terpineol (12.49%). A large proportion of the oil was composed in the groups of hydrocarbons (55.09%) and alcohols (31.21%). In addition, fragrance evaluation of extract of M. kobus flower buds showed the characteristics of balsamic, floral, herbal, medicinal, minty, oily and spicy. In EEG study, the data were observed before and during the inhalation of M. kobus fragrance. During the inhalation of M. kobus fragrance, the EEG power spectrum values of absolute theta and alpha decreased in all the frontal, temporal and parietal regions. A significant decrease (pu2009<u20090.05) of absolute alpha wave was observed at left parietal (P3) region. The results reveal that the changes in EEG values to awaken and increase the concentration states of brain. The present investigation clearly suggested that the fragrance of M. kobus flower buds could be attributed to the enhancement of psychophysiological activities of human.

Evaluation of Herbicidal Potential of Essential Oils and their Components under In vitro and Greenhouse Experiments

The present study aimed to evaluate the phytotoxic potential of essential oils. For this purpose, 18 essential oil samples extracted from Korean plants and 64 commercial essential oils were screened for their phytotoxic potential against the seedling growth of Brassica napus L. (rapeseed). Among the 82 samples, 11 commercial oils (cinnamon, citronella, clove, cumin seed, geranium, jasmine, lemongrass, palmarosa, pimento, rose otto and spearmint) strongly inhibited the seedling growth with GR 50 value −1 . Major components from these effective essential oils were identified by solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS). GC-MS analyses revealed that the effective samples mainly consist of benzyl benzoate, carvone, citral, citronellol, eugenol, geraniol, D-limonene and terpinene. Subsequently, bioactivity of these individual components was evaluated against the seedling growth of B. napus, Echinochloa crus-galli and Aeschynomene indica. The components from different chemical groups exhibited different potency in inhibiting the seedling growth with varied GR 50 values ranged from 29 μg mL −1 to >1,000 μg mL − 1. In the greenhouse experiment, citral and geraniol completely suppressed the growth of all the tested 10 plants at 100 kg ha −1 . In conclusion, the individual essential oil components geraniol and citral could be used as natural herbicides for weed management.