Karina Costa Busato

Meat quality and fatty acid profile of Brazilian goats subjected to different nutritional treatments

This study evaluated the effect of feed restriction and goat genotype on meat quality. Three genotypes (Brazilian native breed Canindé; Brazilian native breed Moxotó; and F1 Boer crossbred animals obtained by crossing Boer bucks with local breed does) and three different feeding regimens (ad libitum fed, AL; restricted fed at 75% of the ad libitum, R.75; or restricted fed at 50% of the average ad libitum intake, R.50) were used. There was no difference (P>0.05) in chemical composition, total and soluble collagen, and shear force of the Longissimus lumborum muscle among genotypes. However, AL had greater amounts of soluble collagen and crude protein in the muscle (P<0.05) than R.75. No difference (P>0.05) was observed for the myofibrillar fragmentation index. The goat genotype presented few differences in their fatty acid profiles. However, goats fed ad libitum had a more favorable fatty acid profile for human health with greater concentrations of oleic acid, unsaturated fatty acids, and conjugated linoleic acid.

Differences in beef quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a proteomic and phosphoproteomic approach

Proteins are the major constituents of muscle and are key molecules regulating the metabolic changes during conversion of muscle to meat. Brazil is one of the largest exporters of beef and most Brazilian cattle are composed by zebu (Nellore) genotype. Bos indicus beef is generally leaner and tougher than Bos taurus such as Angus. The aim of this study was to compare the muscle proteomic and phosphoproteomic profile of Angus and Nellore. Seven animals of each breed previously subjected the same growth management were confined for 84 days. Proteins were extracted from Longissimus lumborum samples collected immediately after slaughter and separated by two-dimensional electrophoresis. Pro-Q Diamond stain was used in phosphoproteomics. Proteins identification was performed using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Tropomyosin alpha-1 chain, troponin-T, myosin light chain-1 fragment, cytoplasmic malate dehydrogenase, alpha-enolase and 78 kDa glucose-regulated protein were more abundant in Nellore, while myosin light chain 3, prohibitin, mitochondrial stress-70 protein and heat shock 70 kDa protein 6 were more abundant in Angus (P<0.05). Nellore had higher phosphorylation of myosin regulatory light chain-2, alpha actin-1, triosephosphate isomerase and 14-3-3 protein epsilon. However, Angus had greater phosphorylation of phosphoglucomutase-1 and troponin-T (P<0.05). Therefore, proteins involved in contraction and muscle organization, myofilaments expressed in fast or slow-twitch fibers and heat shock proteins localized in mitochondria or sarcoplasmic reticulum and involved in cell flux of calcium and apoptosis might be associated with differences in beef quality between Angus and Nellore. Furthermore, prohibitin appears to be a potential biomarker of intramuscular fat in cattle. Additionally, differences in phosphorylation of myofilaments and glycolytic enzymes could be involved with differences in muscle contraction force, susceptibility to calpain, apoptosis and postmortem glycolysis, which might also be related to differences in beef quality among Angus and Nellore.

Technical note: Relationship between infrared thermography and heat production in young bulls

The traditional techniques to measure heat production (HP) are calorimetry (direct and indirect) and comparative slaughter. Both methods are expensive and require extensive amounts of time and infrastructure. Infrared thermography (IRT) could be a faster and less expensive alternative to estimate cattle HP. The objective of this project was to evaluate the use of the IRT technique as an indicator of HP in cattle. A total of 24 bulls (12 Nellore and 12 Black Angus) with initial BW of 380 ± 7 kg were used. Initially, 4 animals of each breed were harvested (baseline animals) and simple regressions were developed for each breed from these baseline animals to estimate the initial chemical composition of the remaining bulls. Eight animals of each breed were fed a silage/concentrate diet for ad libitum intake in individual stalls. On the 25th, 50th, and 75th experimental day, infrared thermal images (Fluke Ti 55ft; Fluke Corporation) were taken of each animals face to access skin and ocular surface temperatures. A metabolism trial was conducted to estimate the ME intake (MEI). After 84 experimental days, the cattle were harvested and retained energy (RE) and HP were calculated. The data were analyzed using the MIXED and REG procedures of SAS adopting a significance level of 0.05. Angus cattle had a greater daily MEI, HP, and skin and eye temperatures than Nellore. We found significant correlations ( ≤ 0.005) between daily HP and maximum ( = 0.65) and average skin temperatures ( = 0.65) and maximum ( = 0.65) and average ocular surface ( = 0.69) temperatures recorded on d 50. Infrared thermography has potential to be used to evaluate HP in cattle.

Technical note: Estimating body weight and body composition of beef cattle trough digital image analysis

The use of digital images could be a faster and cheaper alternative technique to assess BW, HCW, and body composition of beef cattle. The objective of this study was to develop equations to predict body and carcass weight and body fat content of young bulls using digital images obtained through a Microsoft Kinect device. Thirty-five bulls with an initial BW of 383 (±5.38) kg (20 Black Angus, 390 [±7.48] kg initial BW, and 15 Nellore, 377 [±8.66] kg initial BW) were used. The Kinect sensor, installed on the top of a cattle chute, was used to take infrared light-based depth videos, recorded before the slaughter. For each animal, a quality control was made, running and pausing the video at the moment that the animal was standing with its body and head in line. One frame from recorded videos was selected and used to analyze the following body measurements: chest width, thorax width, abdomen width, body length, dorsal height, and dorsal area. From these body measurements, 23 indexes were generated and tested as potential predictors. The BW and HCW were assessed with a digital scale, whereas empty body fat (EBF) was estimated through ground samples of all tissues. To better understand the relationship among the measurements, the correlations between final BW (488 [±10.4] kg), HCW (287 [±12.5] kg), EBF (14 [±0.610] % empty BW) content, body measurements (taken through digital images), and developed indexes were evaluated. The REG procedure was used to develop the regressions, and the important independent variables were identified using the options STEPWISE and Mallows Cp in the SELECTION statement. Chest width was the trait most related to weights and the correlations between this measurement and BW and HCW were above 0.85. The analysis of linear regressions between observed and predicted values showed that all models pass through the origin and have a slope of unity (null hypothesis [H]: = 0 and = 1; ≥ 0.993). The models to estimate BW and HCW of Angus and Nellore presented between 0.69 and 0.84 ( < 0.001), whereas from equations to estimate the EBF were lower ( = 0.43-0.45; ≤ 0.006). Index I5 [(chest width) × body length], related to the animal volume, was significant in all models created to estimate BW and HCW, and it explained more than 70% of the variation. This study indicates that digital images taken through a Microsoft Kinect system have the potential to be used as a tool to estimate body and carcass weight of beef cattle.

Carcass characteristics of zebu steers receiving different oleaginous grains

This research aimed to evaluate carcass traits of Zebu steers fed different oleaginous grains. Thirty one 23 months old zebu steers with 365 + 37.5 kg of live weight were used. The diets consisted ofcorn silage and four different concentrates; no additional lipids and three different ground oilseeds sources: soybean, cottonseed and linseed. The diets had concentrate:roughage ratio of 60:40 and were offered ad libitum. The experimental period was of 84 days, preceded by a 28 day adaptation period. The experiment was carried out in a randomized block design and the means were compared by Scott-Knott test at 5% level of probability. The CS inclusion of into the diet decreased (p < 0.05) carcass yield of the animals. The hindquarter was heavier (p < 0.05) with the addition of SB to the diet. The spare ribs had the highest performance (p < 0.05) and outside flat was heavier (p < 0.05) when the animals were submitted to CS and FS diets. The CS addition to the diet improved carcass characteristics.

Expression of genes related to the regulation of muscle protein turnover in Angus and Nellore bulls.

We aimed to evaluate the expression of genes related to the regulation of muscle protein turnover in the longissimus dorsi (LD) muscle of Angus and Nellore bulls and to estimate the within-breed correlations of gene expression and performance traits. Thirteen genes related to the IGF-1 and myostatin pathways were studied. Thirteen animals, with an initial average BW of 381.2 ± 11.8 kg, from each breed were used in a completely randomized 2 × 2 factorial design (2 breeds and 2 feeding levels). The diet consisted of corn silage and a corn-soybean meal concentrate in a roughage-to-concentrate ratio of 30:70. Cattle were fed ad libitum (with 9 animals from each breed) or feed restricted (a 55% restriction of total DMI of ad libitum-fed animals, calculated as percentage of metabolic BW, with 4 animals of each breed). The experimental period lasted for 82 d and it was preceded by a 28-d adaptation period. The performance traits evaluated were slaughter body weight, total ADG (from d 1 to 82 of the trial), initial ADG (from d 1 to 41 of the trial), final ADG (from d 42 to 82 of the trial), total DMI (from d 1 to 82 of the trial), initial DMI (from d 1 to 41 of the trial), final DMI (from d 42 to 82 of the trial), HCW, LD weight (LDW), and rib eye area (REA). After slaughter, samples were taken from the LD muscle between the 12th and 13th ribs for gene expression analysis by quantitative reverse transcription PCR. There was no difference ( > 0.05) in the expression of any of the genes studied between ad libitum-fed Angus and ad libitum-fed Nellore, whereas feed restriction increased the expression of (; < 0.001), (; = 0.05), and (; = 0.04) and decreased the expression of ( < 0.01). The REA was negatively correlated to (; = 0.01), (; = 0.02), and ( = 0.05). The HCW was negatively correlated to ( = 0.01) and ( = 0.01) and tended to be negatively correlated to ( = 0.07), whereas the LDW tended to be negatively correlated to ( = 0.08). The genes , , and seem to be important for muscle growth and may be worthy of further investigation as future strategies for increasing muscle in livestock.

Características de carcaça de novilhos zebuínos recebendo diferentes grãos de oleaginosas

This research aimed to evaluate carcass traits of Zebu steers fed different oleaginous grains. Thirty one 23 months old zebu steers with 365 + 37.5 kg of live weight were used. The diets consisted ofcorn silage and four different concentrates; no additional lipids and three different ground oilseeds sources: soybean, cottonseed and linseed. The diets had concentrate:roughage ratio of 60:40 and were offered ad libitum. The experimental period was of 84 days, preceded by a 28 day adaptation period. The experiment was carried out in a randomized block design and the means were compared by Scott-Knott test at 5% level of probability. The CS inclusion of into the diet decreased (p < 0.05) carcass yield of the animals. The hindquarter was heavier (p < 0.05) with the addition of SB to the diet. The spare ribs had the highest performance (p < 0.05) and outside flat was heavier (p < 0.05) when the animals were submitted to CS and FS diets. The CS addition to the diet improved carcass characteristics.

Carcass characteristics of zebu steers receiving different oleaginous grains - doi: 10.4025/actascianimsci.v35i3.14533

This research aimed to evaluate carcass traits of Zebu steers fed different oleaginous grains. Thirty one 23 months old zebu steers with 365 + 37.5 kg of live weight were used. The diets consisted ofcorn silage and four different concentrates; no additional lipids and three different ground oilseeds sources: soybean, cottonseed and linseed. The diets had concentrate:roughage ratio of 60:40 and were offered ad libitum. The experimental period was of 84 days, preceded by a 28 day adaptation period. The experiment was carried out in a randomized block design and the means were compared by Scott-Knott test at 5% level of probability. The CS inclusion of into the diet decreased (p < 0.05) carcass yield of the animals. The hindquarter was heavier (p < 0.05) with the addition of SB to the diet. The spare ribs had the highest performance (p < 0.05) and outside flat was heavier (p < 0.05) when the animals were submitted to CS and FS diets. The CS addition to the diet improved carcass characteristics.

Digestibilidade de Cordeiros em Diferentes Níveis de Oferta de Alimentos

Objetivou-se avaliar o consumo e a digestibilidade de cordeiros sem raca definida (SRD) (45 animais), em diferentes classes sexuais (15 machos inteiros, 15 machos castrados e 15 femeas), mantidos em diferentes niveis de alimentacao (a vontade, 25 e 50% de restricao). A dieta foi constituida de capimelefante e concentrado com relacao volumoso:concentrado de 40:60. Nao houve diferencas (P>0,05) entre as classes sexuais e nao ocorreu interacao (P>0,05) da classe sexual com os niveis de alimentacao para as variaveis analisadas. Por outro lado, houve efeito da restricao alimentar sobre esses parâmetros (P<0,05). A restricao alimentar reduziu os valores de consumo e elevou os coeficientes de digestibilidade da materia seca e dos nutrientes. DOI: 10.15528/2176-4158/rcpa.v14n1p73-76