Kasturi Das

How “suspicious” is that nodule? Review of “suspicious” Afirma gene expression classifier in high risk thyroid nodules

The Afirma gene expression classifier (GEC) is being increasingly utilized to confirm the benign nature of indeterminate FNA cytology results thus avoiding unnecessary surgical procedures. However the “suspicious” result of the Afirma GEC does not classify these indeterminate nodules further in determining appropriate management. This study investigated the outcome of the thyroid nodules deemed to be “suspicious” by the Afirma GEC in a high risk population.

The impact of subdividing the "atypical" category for urinary cytology on patient management.

The purpose of the study is to determine the impact of subdividing the “atypical” cytology interpretation into two groups: Atypical urothelial cells of uncertain significance (AUC‐US) and Atypical urothelial cells suspicious for high‐grade urothelial carcinoma (AUC‐H/SHGUC), on management of patients with no prior history of UC.

Lack of transformation zone in cervical Pap tests, should it be a concern? A quality assurance initiative

Bethesda guidelines do not require presence of transformation zone (TZ) for a cervical Pap test to be deemed adequate. However, clinicians are concerned with specimens that are reported to lack TZ.

Cytology smears for DNA extraction: Practical approach for selecting the best slide

Next generation sequencing (NGS) to detect actionable genetic abnormalities is standard of care in advanced stage lung adenocarcinoma. Many studies have shown that the molecular results obtained from fine needle aspiration cytology material are comparable to those obtained from formalin‐fixed tissue samples. We undertook this study to validate DNA extraction from cytology material for molecular studies and to find any correlation between DNA yield, pattern of tumour cells and tumour fraction.

Pleural effusion in a 7-year-old child - with unique cells

DOI: 10.4103/cytojournal. cytojournal_13_17 A 7‐year‐old male presented with 2‐month history of fatigue, pallor, and weight loss and 1‐month history of fevers and mucositis. He then developed rash in the form of small papules on his face, hands, and feet, diagnosed to be viral infection. He was started on acyclovir. He continued to be ill with an erythematous rash on his face and hands with intermittent cough. Computed tomography scan of the chest revealed multilobar bilateral foci of pulmonary consolidation and diffuse adenopathy. Later, he acutely developed respiratory distress and was found to have a moderate‐to‐large right‐sided pleural effusion. Thoracocentesis yielded yellow cloudy fluid with elevated cell count and neutrophils, consistent with an exudate. Culture of the fluid grew streptococcus pneumoniae bacteria. Blood culture was negative.