Kate Hardy
Hammersmith Hospital
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Publication
Featured researches published by Kate Hardy.
Biology of Reproduction | 2003
Kate Hardy; Jaroslav Stark; Robert M.L. Winston
Abstract The degree of fragmentation during early cleavage is universally used as an indicator of embryo quality during human in vitro fertilization treatment. Extensive fragmentation has been associated with reduced blastocyst formation and implantation. We examined the relationship between early fragmentation and subsequent allocation of cells to the trophectoderm and inner cell mass in the human blastocyst. We retrospectively analyzed data from 363 monospermic human embryos that exhibited varying degrees of fragmentation on Day 2. Embryos were cultured from Day 2 to Day 6 in Earle balanced salt solution with 1 mM glucose and human serum albumin. Rates of development and blastocyst formation were measured. The number of cells in the trophectoderm and inner cell mass and the incidence of apoptosis were assessed following differential labeling with polynucleotide-specific fluorochromes. Increasing fragmentation resulted in reduced blastocyst formation and lower blastocyst cell numbers. For minimal and moderate levels of fragmentation, the reduction in cell numbers was confined largely to the trophectoderm and a steady number of inner cell mass cells was maintained. However, with extensive fragmentation of more than 25%, cell numbers in both lineages were reduced in the few embryos that formed blastocysts. Apoptotic nuclei were present in both the trophectoderm and inner cell mass, with the lowest incidence in blastocysts that had developed from embryos with minor (5–10%) fragmentation. Paradoxically, higher levels of apoptosis were seen in embryos of excellent morphology, suggesting a possible role in regulation of cell number.
Proceedings of the National Academy of Sciences of the United States of America | 2001
Kate Hardy; Sophie Spanos; David L. Becker; P. Iannelli; Robert M.L. Winston; Jaroslav Stark
Reproduction | 2002
Sophie Spanos; Suman Rice; P. Karagiannis; Deborah Taylor; David L. Becker; Robert M.L. Winston; Kate Hardy
Society for Endocrinology BES 2010 | 2010
Fabio Comim; Sharron A. Stubbs; Kate Hardy; Stephen Franks
20th European Congress of Endocrinology | 2018
Lisa Owens; Ali Abbara; Avi Lerner; Georgios Christopoulos; Shirin Khanjani; Rumana Islam; Maneshka Liyanage; Kate Hardy; Stuart Lavery; Aylin C. Hanyaloglu; Waljit Dhillo; Stephen Franks
20th European Congress of Endocrinology | 2018
Lisa Owens; Georgios Christopolous; Shirin Khanjani; Kate Hardy; Stuart Lavery; Stephen Franks; Aylin C. Hanyaloglu
Society for Endocrinology BES 2016 | 2016
Lisa Owens; Avi Lerner; Silvia Sposini; George Christopoulos; Shiran Khanjani; Razia Islam; Stuart Lavery; Vicky Tsui; Kate Hardy; Stephen Franks; Aylin C. Hanyaloglu
Society for Endocrinology BES 2016 | 2016
Kacie Thomson; Victoria Atess; Mhairi Laird; Stephen Franks; Kate Hardy
Society for Reproduction and Fertility Annual Conference 2015 | 2015
Sofia Granados-Aparici; Isam Sharum; Kate Hardy; Stephen Franks; Sarah L. Waite; Neil Chapman; Mark A. Fenwick
Society for Reproduction and Fertility Annual Conference 2015 | 2015
Jocelyne Velupillai; Avi Lerner; Georgios Christopoulos; Maneshka Liyanage; Stuart Lavery; Geoffrey Trew; Mhairi Laird; Jocelyn M. Mora; Victoria Tsui; Kate Hardy; Stephen Franks