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Dive into the research topics where Katsuaki Endou is active.

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Featured researches published by Katsuaki Endou.


Journal of Immunology | 2003

c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages: role of mitogen-activated protein kinase in glutathione redox regulation of IL-12 production.

Mitsuyoshi Utsugi; Kunio Dobashi; Tamotsu Ishizuka; Katsuaki Endou; Junji Hamuro; Yukie Murata; Tsugio Nakazawa; Masatomo Mori

Although c-Jun N-terminal kinase (JNK) plays an important role in cytokine expression, its function in IL-12 production is obscure. The present study uses human macrophages to examine whether the JNK pathway is required for LPS-induced IL-12 production and defines how JNK is involved in the regulation of IL-12 production by glutathione redox, which is the balance between intracellular reduced (GSH) and oxidized glutathione (GSSG). We found that LPS induced IL-12 p40 protein and mRNA in a time- and concentration-dependent manner in PMA-treated THP-1 macrophages, and that LPS activated JNK and p38 mitogen-activated protein (MAP) kinase, but not extracellular signal-regulated kinase, in PMA-treated THP-1 cells. Inhibition of p38 MAP kinase activation using SB203580 dose dependently repressed LPS-induced IL-12 p40 production, as described. Conversely, inhibition of JNK activation using SP600125 dose dependently enhanced both LPS-induced IL-12 p40 production from THP-1 cells and p70 production from human monocytes. Furthermore, JNK antisense oligonucleotides attenuated cellular levels of JNK protein and LPS-induced JNK activation, but augmented IL-12 p40 protein production and mRNA expression. Finally, the increase in the ratio of GSH/GSSG induced by glutathione reduced form ethyl ester (GSH-OEt) dose dependently enhanced LPS-induced IL-12 p40 production in PMA-treated THP-1 cells. GSH-OEt augmented p38 MAP kinase activation, but suppressed the JNK activation induced by LPS. Our findings indicate that JNK negatively affects LPS-induced IL-12 production from human macrophages, and that glutathione redox regulates LPS-induced IL-12 production through the opposite control of JNK and p38 MAP kinase activation.


Immunology Letters | 2002

Toll-like receptor 4 surface expression on human monocytes and B cells is modulated by IL-2 and IL-4

Yoshinori Mita; Kunio Dobashi; Katsuaki Endou; Tadayoshi Kawata; Yasuo Shimizu; Tsugio Nakazawa; Masatomo Mori

Human Toll-like receptor 4 (TLR4) has recently been identified, and it has been shown to be the main protein involved in recognizing gram-negative bacteria. We examined the regulation of TLR4 surface expression in human peripheral blood monocytes and B cells by interleukin-2 (IL-2) and IL-4. IL-2 up-regulated TLR4 surface expression on human peripheral blood monocytes, but did not change expression on human peripheral B cells. By contrast, IL-4 down-regulated TLR4 surface expression on human peripheral blood monocytes, but up-regulated TLR4 surface expression on human peripheral B cells. These results indicate that Th1 cytokine IL-2 enhances receptors involved in the response to gram-negative bacteria and that activation of cellular immunity may enhance defense against these pathogens through monocytes, but not B cells, whereas Th2 cytokine IL-4 modulates the receptor response to gram-negative bacteria and that activation of humoral immunity may enhance defense against these pathogens through B cells, but not monocytes.


Journal of Clinical Biochemistry and Nutrition | 2008

Five-antituberculosis Drug-resistance Genes Detection Using Array System

Yasuo Shimizu; Kunio Dobashi; Yoko Yoshikawa; Shigeki Yabe; Seiichi Higuchi; Yoko Koike; Yoshihiro Mita; Mitsuyoshi Utsugi; Katsuaki Endou; Kouichi Takahashi; Satoru Watanabe; Ryusei Saito; Masatomo Mori

Detection of resistance to drugs for Mycobacterium tuberculosis takes about two months from the sample collection using culture-based methods. To test a rapid method for detection of resistance for five antituberculosis drugs using DNA microarray and to examine its potential for clinical use, we employed a DNA microarray for detection of seven mutations genes related to resistance of five kinds of antituberculous drugs using Mycobacterium tuberculosis DNA isolated from sputum. The results of microarray analysis were compared with the results of a standard culture method of Lowenstein-jensen drug sensitivity testing system. DNA microarray analysis showed a high sensitivity (>90%) for all five drugs. Specificity of rifampicin and ethambutol were nearly 90%, however specificity of isoniazid (60%) and kanamycin (67%) were not enough. The amount of Mycobacterium tuberculosis DNA required for microarray analysis corresponded to at least 1–9 Acid-Fast Bacilli per 10 fields by carbolfuchsin staining. DNA microarray analysis appears to be useful for estimation of drug resistances, nevertheless its limitations. To minimize misunderstanding, it is necessary to confirm the number of bacilli in the sputum, and culture method is needed for comparison when use the PCR-based array system.


European Journal of Pharmacology | 2002

Ambroxol inhibits platelet-derived growth factor production in human monocytic cells.

Mitsuyoshi Utsugi; Kunio Dobashi; Yasuhiko Koga; Ken Masubuchi; Yasuo Shimizu; Katsuaki Endou; Tsugio Nakazawa; Masatomo Mori

Several growth factors, including platelet-derived growth factor (PDGF), have been implicated in the mechanism of lung and airway remodeling. We investigated the effect of ambroxol, trans-4-[(2-amino-3,5-dibromobenzyl) amino] cyclohexanol hydrochloride, on the lipopolysaccharide-induced PDGF production in human monocytic cells, THP-1. Ambroxol inhibited the lipopolysaccharide-induced PDGF-AB production via PDGF-A mRNA expression. Lipopolysaccharide activated p44/42 extracellular signal-regulated kinase (ERK), and ambroxol attenuated the lipopolysaccharide-induced p44/42 ERK activation. Furthermore, mitogen-activated protein kinase kinase (MEK)-1-specific inhibitor, 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD 98059), blocked the lipopolysaccharide-induced p44/42 ERK activation and PDGF production. These findings indicate that ambroxol inhibits the lipopolysaccharide-induced PDGF production due to the suppression of p44/42 ERK activity.


Cases Journal | 2009

Multiple lung metastases presenting as ground-glass opacities in a pulmonary adenocarcinoma: a case report.

Noriko Yanagitani; Kyoichi Kaira; Tamotsu Ishizuka; Haruka Aoki; Mitsuyoshi Utsugi; Yasuo Shimizu; Noriaki Sunaga; Katsuaki Endou; Takeshi Hisada; Masatomo Mori

IntroductionFocal ground-glass opacity on computed tomography suggests several disorders including inflammatory disease, fibrosis, or a primary lung neoplastic lesion, metastatic lung tumor.Case presentationThe case of a 55-year-old female presenting with adenocarcinoma of the lung is herein reported. Computed tomography of the chest revealed a primary mass lesion in the upper lobe of the right lung and multiple metastases presenting as ground-glass opacities. Macroscopic metastases were observed in the bone, the hilar and mediastinal lymph nodes, and another lobe. This case was advanced lung cancer. We assumed that the multiple ground-glass opacity lesions were metastasis in the lungs. Chest CT revealed a partial response of the primary site and the multiple ground-glass opacities after systemic chemotherapy.ConclusionA metastatic lung tumor showing ground-glass opacity is uncommon. It is quite difficult to distinguish between multiple primary lung cancers and intrapulmonary metastasis when patients present with multiple lung nodules. A lot of clinical information is therefore required to make an accurate diagnosis in such cases.


Inflammation | 2005

Safety and Efficacy of Extracorporeal Granulocyte and Monocyte Adsorption Apheresis in Patients with Severe Persistent Bronchial Asthma

Tamotsu Ishizuka; Tadayoshi Kawata; Yasuo Shimizu; Mitsuyoshi Utsugi; Katsuaki Endou; Takeshi Hisada; Kunio Dobashi; Tsugio Nakazawa; Masatomo Mori

The Adacolumn is an adsorptive-type extracorporeal device, which is filled with cellulose diacetate beads that selectively adsorb granulocytes and monocytes. Patients with severe persistent asthma experience highly variable continuous symptoms and severe exacerbations in spite of medication based on inhaled glucocorticosteroids. Granulocyte and monocyte adsorption apheresis using extracorporeal circulation through the Adacolumn was performed in nine patients with severe persistent asthma. The extracorporeal circulation through the Adacolumn was performed once a week for 5 weeks. We were able to perform this therapy without any severe adverse effects in all patients, although one patient complained of general fatigue just after the circulations. In six of the nine patients, the increase in peak expiratory flow (PEF) was more than 50 mL/min. The average increase in morning PEF was 23.3% while that in the evening PEF was 26.4% after the therapy. This therapy was not harmful for patients with severe persistent asthma. A placebo-controlled study will be desired to evaluate the efficacy of this nonpharmacological strategy accurately.


Tuberculosis | 2006

DNA microarray genotyping of N-acetyltransferase 2 polymorphism using carbodiimide as the linker for assessment of isoniazid hepatotoxicity

Yasuo Shimizu; Kunio Dobashi; Yoshihiro Mita; Katsuaki Endou; Shogo Moriya; Kaoru Osano; Yoko Koike; Seiichi Higuchi; Shigeki Yabe; Mitsuyoshi Utsugi; Tamotsu Ishizuka; Takeshi Hisada; Tsugio Nakazawa; Masatomo Mori


American Journal of Physiology-lung Cellular and Molecular Physiology | 2004

8-Bromo-cAMP decreases the Ca2+ sensitivity of airway smooth muscle contraction through a mechanism distinct from inhibition of Rho-kinase

Katsuaki Endou; Kunihiko Iizuka; Akihiro Yoshii; Hideo Tsukagoshi; Tamotsu Ishizuka; Kunio Dobashi; Tsugio Nakazawa; Masatomo Mori


The Kitakanto Medical Journal | 2005

Rapid Effect of nCPAP Therapy on Circulating Plasma Leptin in OSAS Patients

Yasuo Shimizu; Kunio Dobashi; Takeo Horie; Yasuhiko Koga; Akihiro Yoshii; Mitsuyoshi Utsugi; Katsuaki Endou; Hiroyuki Shimizu; Kenjyu Shimomura; Takafumi Tsuchiya; Yoshinori Mita; Tadayoshi Kawata; Kunihiko Iizuka; Tsugio Nakazawa; Masatomo Mori


American Journal of Respiratory and Critical Care Medicine | 2012

Massive Pleural Effusion Due to Pleural AA Amyloidosis

Yasuo Shimizu; Katsuaki Endou; Yutaka Hashizume; Tadayoshi Kawata; Takeo Horie; Hidemi Ogura; Masaru Obokata; Mitsuo Toyoda; Hideaki Ito; Atsushi Takise

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