Kazumi Yamasaki

Metabolic studies of bile acids. XLV1. The transformation of 3β,-7α-dihydroxychol-5-enic-24-14C acid to chenodeoxycholic acid in the rat. The significance of the C-7α-hydroxyl group in bile acid formation

Abstract When 7α,12α-dihydroxy-3-oxocholanic-12β- 3 H acid and 7α-hydroxy-3-oxocholanic-7β- 3 H acid were administered to rats furnished with bile fistulas, cholic acid and chenodeoxycholic acid, respectively, were excreted in their bile. In addition, 3β,7α-dihydroxychol-5-enic-24- 14 C acid was converted to chenodeoxycholic acid. The significance of the C-7α-hydroxyl group in bile acid formation from cholesterol is discussed.

Metabolism of 7β-Hydroxycholesterol-4-14C in rat

7β-Hydroxycholesterol-14C was administered to bile-fistula rats, whose bile was collected and analyzed. As the major metabolites of this hydroxy sterol, the following unsaturated C24-bile acids were identified: the 4 possible stereoisomers of 3,7-dihydroxychol-5-enoic acid as well as those two of 3-hydroxy-7-oxochol-5-enoic acid. In addition, cheno- and ursodeoxycholic acids were indentified as minor metabolites. The meaning of these findings was discussed.