Toshiaki Usui

Fluorimetric determination of serum 11-hydroxysteroids. Calculative correction of deviations in complex fluorescence spectra

Abstract Fluorimetry of serum 11-hydroxycorticosteroids was accompanied by contamination due to nonspecific fluorescence. A spectro-fluorometrical method was proposed for the correction of the contamination.

Metabolic studies of bile acids. XLV1. The transformation of 3β,-7α-dihydroxychol-5-enic-24-14C acid to chenodeoxycholic acid in the rat. The significance of the C-7α-hydroxyl group in bile acid formation

Abstract When 7α,12α-dihydroxy-3-oxocholanic-12β- 3 H acid and 7α-hydroxy-3-oxocholanic-7β- 3 H acid were administered to rats furnished with bile fistulas, cholic acid and chenodeoxycholic acid, respectively, were excreted in their bile. In addition, 3β,7α-dihydroxychol-5-enic-24- 14 C acid was converted to chenodeoxycholic acid. The significance of the C-7α-hydroxyl group in bile acid formation from cholesterol is discussed.

A new method for estimation of corticosteroid-binding globulin with hydrophobic resin

Abstract A new method is described for the estimation of corticosteroid-binding globulin by means of the hydrophobia resin adsorption method. Corticosteroid-binding globulin in the serum is saturated by incubation with cortisol, unbound and albumin-bound cortisol is removed by shaking with the resin, and cortisol remaining in the supernatant fluid is measured by fluorometry, its represents the cortisol-binding capacity of corticosteroid-binding globulin. Normal range of cortisol-binding capacity was 0.154–0.222 μg/ml at 15°.