Kazusada Yoshitake

Increased β2-microglobulin in both parotid and submandibular/sublingual saliva from patients with Sjögren's syndrome

Abstract Samples of unstimulated saliva from patients with sialoadenopathy were collected by microcapillary tube (1 μl), and their β 2 -microglobulin (B2-MG) content determined by a sandwich enzyme immunoassay. A higher than normal (control) concentration of the globulin was present in both parotid and submandibular/sublingual saliva from the patients with Sjogrens syndrome but not in the samples from the patients with sialoadenitis or diabetes mellitus. The increase in B2-MG in saliva from patients with Sjogrens syndrome may reflect that immunolopathological events are important in the degeneration of both glands in this disease. Therefore, the determination of B2-MG in saliva may be a simple, non-invasive technique for confirming the diagnosis of Sjogrens syndrome as an autoimmune disease.

Distribution and forms of fluorine in whole blood of human male

Distribution and absolute concentration of three kinds of fluoride/fluorine, i.e., free ionic, ionizable, and organic fluorine, in human whole blood and serum were determined. The method is based on low temperature oxygen plasma ashing, acid hydrolysis, gas chromatography using chlorotrimethylsilane, and fluoride ion-specific electrode. A large part (40%) of the total F is distributed in clot of the whole blood and 74% of the total F is in the form of organic fluorine.

Gas chromatographic determination of traces of fluoride with several alkylsilane extractants

Abstract A comparative study of the determination of fluoride at the natural abundance level by formation of fluoroalkylsilanes, extraction and gas chromatography is reported. The reagents tested were the chloro and imidazole derivatives of trimethyl-, ethyldimethyl-, isopropyldimethyl- and tert-butyldimethyl-silane and chlorotriethylsilane. Fluorotriethylsilane (FTES) derived from chlorotriethylsilane provided twice the sensitivity obtained with fluorotrimethylsilane, the most commonly used derivative, whether the chloro or imidazole reagent was used. The reaction time required for the triethyl derivative was longer (90 min) than for the trimethyl derivative (5 min). The precision of the method with FTES was within 5% for 10–50 ng of fluoride. Guidelines on the choice of an appropriate alkylsilane compound and detailed conditions for the determination of fluoride in blood serum are described.

Two-dimensional electrophoresis of human salivary proteins from patients with sialoadenopathy

Unstimulated saliva was fractionated by micro two-dimensional gel electrophoresis, and the proteins visualized by silver staining and immunostaining. The subjects with Sjögrens syndrome exhibited both quantitative and qualitative alterations in the protein composition of the saliva not only from the parotid gland but also from the submandibular/sublingual glands.

Granular cell ameloblastoma. A rare variant

A rare case of granular cell ameloblastoma in the anterior mandible of a 59-year-old man has been studied by light and electron microscopy. In some areas, the tumor was very similar to an oncocytoma (oxyphilic adenoma). Almost all tumor cells were full of eosinophilic granules, whereas in the common type of granular cell ameloblastoma, only the cells located in the central portion of the tumor are granular.

Mechanism of Fluoride Absorption from the Gastrointestinal Tract in Rats

Abstract The mechanism involved in the absorption of fluoride by the gastrointestinal tract has been studied by the gastrointestinal reflux method in situ of rat. Reflux was applied to the stomach, small intestine, rectum, and mouth of each animal. At certain intervals during the reflux, a portion of the reflux solution was taken for determination of fluoride uptake. When the stomach or the small intestine was refluxed for 1 hour, the rate of fluoride absorption was decreased with increase in fluoride concentrations, and in the presence of dinitrophenol or saline. These results indicate that the uptake of fluoride from the gastrointestinal tract of the rat may not be limited to passive transport alone.

One and three year prospective study of treatment of temporomandibular joint dysfunction by intraoral vertical ramus osteotomy

The purpose of this study was to determine if the intraoral vertical ramus osteotomy is an effective treatment for anterior disk displacements with reduction and associated temporomandibular joint pain and dysfunction. Success with this procedure was predicated on producing a more functional articular disk-condyle relationship by anterior and inferior repositioning of the condyle. The prospective study was undertaken of nine (eight female and one male) patients (mean age of 25.8 years) with anterior disk displacement with reduction and associated temporomandibular joint pain and dysfunction (mean duration of pain of 6.8 years). History, clinical dysfunction, and pain indices were used to determine the frequency and severity of temporomandibular joint dysfunction. The incidence of ear pain, muscle fatigue, tinnitus, headaches, and clicking was also assessed. The severity of the pain experience in the masticatory muscles and temporomandibular joints was evaluated through the use of a pain index. The severity of the pain experience and temporomandibular joint dysfunction in all of the patients was significantly reduced or totally eliminated during the period of followup. Surgical treatment of anterior disk displacement with reduction by intraoral vertical ramus osteotomy resulted in improved temporomandibular joint function and resolution of symptoms.

Upper lip cancer in patient with depression. Report of a case.

鬱病を有する45歳の男性に生じた上唇癌の1例を経験した。まずCPE化学療法 (Cisplatin, Peplomycine, Etoposide) を1コース行った後, 上唇腫瘍の外科的切除を行った。腫瘍切除後に生じる上唇の欠損は口唇幅径の約2/5であったが, 皮弁による再建は患者の同意が得られなかったため, やむなく縫縮し術後に放射線照射 (60Gy) を行った。本例は治療を進めていく途中に, 鬱病の悪化による抑鬱, 焦燥, 不安状態により不眠や排尿困難などの身体症状の訴えが何度も認められ, その対応に大変苦慮した症例であった。術後1年6ヶ月経過した現在, 再発傾向は認められず, また口唇の大きな変形, 摂食・発語・開閉口時の機能障害などの後遺症は認められず経過良好である。

Surgical Correction of Severe Mandibular Protrusion by Ramus Osteotomy Combined with Mandibular Anterior Segmental Osteotomy

Surgical correction of severe mandibular protrusion had been frequently performed by the Obwegeser II surgical procedure or combined mandibular osteotomy. Although clinically acceptable results had been achieved by applying the former method, certain problems such as intraoperative complication and postoperative stability had not been completely solved. The latter method had been considered to solve the problems. There were few articles discussing it, despite combined mandibular osteotomy used for the treatment of mandibular deformity in Japan.The purpose of this paper was to discuss the clinical problems of the Obwegeser II method and to elucidate the surgical criteria for producing better results.Case 1 was a 22-year-old man, who had protrusion of the upper lip and mandible. W e treated him with maxillary anterior segmental osteotomy and sagittal splitting ramus osteotomy combined with mandibular anterior segmental osteotomy.Case 2 was a 20-year-old woman, who had severe mandibular protrusion. We treated her with intraoral vertical ramus osteotomy combined with mandibular anterior segmental osteotomy.Results of both cases were excellent, and literature was cited in our report.