Ken Nishida

Serum Proteins and Secretory Component in Human Carious Dentin

By the use of the peroxidase-labeled antibody method, significant localization of IgG, IgA, albumin and transferrin was demonstrated in the deep lesion of 20 carious teeth, where the secretory component was absent. These serum proteins formed a distinct zone, surrounding the overlying, shallow lesion infected with bacteria.

Thermal degradation of sugars in a gas chromatographic injection port

Abstract The degradation products of glucose formed during gas chromatographic analysis of fermentation liquids of Streptococcus mutans, S. mitis and S. salivarius were investigated. A total of 18 sugars from mono- to trisaccharides were also examined by direct injection of aqueous solutions of the carbohydrates into a gas chromatograph packed with porous polymer Chromosorb 101. The main degradation products, including acetic acid and furfural derivatives, were identified by gas chromatography-mass spectrometry.

Monoclonal Antibodies Against Gingival Components

The aim of this study was to produce and characterize monoclonal antibodies against human gingival epithelial cells and gingival fibroblasts. By using these whole cells as immunogens, we were able to generate a large number of monoclonal antibodies reacting with tissue antigens, in particular antibodies that reacted with desmosomes (MoAbs 7 and 8) and basement membrane (MoAb FB-1) antigens. MoAbs 7 and 8 produced from epithelial cells stained cell membranes of epithelium and desmosomes, respectively, as shown by light and immunoelectron microscopy. The epitopes to which MoAbs 7 and 8 were reactive were stable against various treatments; only periodate oxidation abolished the tissue reactions with MoAb 8. Extraction of gingiva with SDS or NP-40, SDS-PAGE analysis, and Western blotting showed that the MoAb 8 identified an antigen with a molecular weight of 100,000 daltons. MoAb FB-1 produced from fibroblasts immunolabeled the basement membranes. The FB-1 antigen was clearly different from any of the known ubiquitous basement membrane components, such as type IV collagen, laminin, and fibronectin. Examination of the distribution and localization of the antigen showed that it was present in the basement membrane beneath stratified squamous epithelium. FB-1 did not react with any of types I-VI collagens in ELISA, but immunoelectron microscopy showed that the antigen reacting with FB-1 was present in the lamina fibroreticularis of the basement membrane and was comprised of collagen-like fibers. These results suggest the possible existence of a new collagen other than types I-VI in the basement membrane beneath stratified squamous epithelium.

An immunopathological study of periodontal disease. Chronic sensitization by oral bacteria and periodontitis

辺縁性歯周炎の発症と経過に免疫現象が関与することが明らかにされている。その経過にみられる免疫現象は, 口腔内細菌による遷延感作あるいはより複雑な機序に基づくかもしれない。著者らはヒトロ腔内細菌を用いてモルモットを遷延感作したところ, 特異抗体産生は, 3ヵ月後に最高に達し, それ以降は漸時減少した。また, 辺縁性歯周炎罹患者血清中のヒトロ腔内細菌に対する特異抗体と免疫グロブリン (IgG) 含有量, さらに自己歯肉組織に対する特異抗体が存在するかどうかを健常者血清中のそれらと比較して検索した結果, 細菌特異抗体量は平均値では増加の傾向を示すが, 極端な最高ならびに最低値の症例を除くと減少の傾向を, しかし免疫グロブリン (IgG) 含有量は増加を示した。そして歯肉組織に対する特異抗体は全ての症例において検出されなかった。これらのことから, 本疾患の経過中に動物実験でみられたような遷延感作成立の可能性は否定的であった。