Ken Sakai

Enzyme-linked immunosorbent assay (ELISA) for human epidermal growth factor (hEGF)

An enzyme-linked immunosorbent assay for human epidermal growth factor (hEGF) by the sandwich method with the use of orthophenylene diamine (OPDA) for the substrate of the enzyme reaction was developed. This assay showed high sensitivity, and there was no problem with the recovery rates even without any pretreatment of samples. The changes of hEGF levels in plasma and serum depend on the condition of blood storage from sampling until separation of the cell content. The levels of hEGF markedly increased with time in serum, especially when stored at room temperature but less so in plasma. Changes of hEGF levels in plasma were negligible for up to 3 h if the blood was stored at 4 degrees C until separation.

MALIGNANT FIBROUS HISTIOCYTOMA SHOWING CYTOPLASMIC HYALINE GLOBULES AND STROMAL OSTEOIDS: A Case Report with Light and Electron Microscopic, Histochemical, and Immunohistochemical Study

A 56‐year‐old man died of widespread metastases of malignant fibrous histiocytoma (MFH) primarily developed in the left lower gingiva after a rapid clinical course is reported. Primary and metastatic tumors showed light and electron microscopic, histochemical and immunohistochemical characteristics of MFH together with cytoplasmic hyaline globules and stromal osteoids. Hyaline globules revealed periodic acid‐Schiff positive and diastase resistant reactions but not positive with any histochemical and immunohistochemical reactions. Osteoids were partially calcified and rimmed by osteoblastic tumor cells. The present case of MFH may provide difficulties in the differential diagnosis from some osteoid‐forming sarcomas in soft tissues with relation to the histogenesis.

Chronic pancreatitis with common bile duct stricture - Studies on clinical findings and operative procedures.

胆管狭窄をともなう慢性膵炎6例を検討した.1) 黄疸は4例でみられ, うち3例は自然消退したが, 1例は胆管完全閉塞に対し経皮的胆管ドレナージを要した. 2) 胆管炎・胆嚢炎は5例, 胆石は3例に認められ, 胆汁細菌培養は60%で陽性であった. 3) 胆管像は全例で総胆管拡張をともない, 膵内胆管しめつけ2例, 不整狭窄2例, 圧排1例, 完全閉塞1例が認められた. 4) 胆道・膵同時バイパス4例 (Double Roux-Y2, ρloop, Roux-Y 1, 胆管十二指腸吻合兼嚢胞空腸吻合1例), 胆管十二指腸吻合1例 (空腸間置法), 膵十二指腸切除1例を行い良好な成績を得た. 以上より胆管狭窄をともなった慢性膵炎は胆汁うっ滞・胆道系炎症を高率に合併するため, 症例に応じた適切な胆道再建術を要するものと考えられた.

SPLENIC AUTOTRANSPLANTATION IN THE OPERATION FOR THE GASTRIC CANCER

胃癌に対する胃全摘兼摘脾を施行した4例に対し, 平均21%重量の脾自家移植により脾温存を試み以下の成績を得た. 1) 脾自家移植にともなう合併症は認められなかった. 2) 術後の脾シンチで全例に生着を確認し, 移植脾による網内系貧食能が認められた. 3) 術後血清IgMの低下は認められず, 縫合不全例では, 著しい上昇を認めた. 4) 術後血小板は一過性に増加したが, その後術前値に復した. 以上本法による胃癌手術は, 癌の根治切除の原則を満たしつつ脾機能を温存するという安全で有用な術式と考えられる.