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Kenichi Miyoshi

City of Hope National Medical Center

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Publication

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Biotechnology Letters | 1989

Overproduction of human insulin-like growth factor-II inEscherichiacoli

Fusakazu Misoka; Tetsuo Miyake; Kenichi Miyoshi; Masanori Sugiyama; Shunji Sakamoto; Toru Fuwa

SummaryHuman insulin-like growth factor II (hIGF-II) was produced inEscherichiacoli as a protein fused to human growth hormone. High level expression of the fusion protein was attained with pIBL-1 plasmid. The hIGF-II obtained byinvitro cleavage of the fusion protein with cyanogen bromide was highly purified and its biological activity was assessed.

Tetrahedron Letters | 1979

Solid phase synthesis of nonadecathymidylic acid by the phosphotriester approach

Kenichi Miyoshi; Keiichi Itakura

Abstract Nonadecathymidylic acid was synthesized by the block coupling phosphotriester approach on a polymer support.

Archive | 1987

Oligonucleotide derivatives and production thereof

Kenichi Miyoshi; Toru Fuwa

Proceedings of the National Academy of Sciences of the United States of America | 1985

Synthesis and secretion of human epidermal growth factor by Escherichia coli

Takanori Oka; S. Sakamoto; Kenichi Miyoshi; Toru Fuwa; K. Yoda; M. Yamasaki; G. Tamura; Tetsuo Miyake

Nucleic Acids Research | 1980

Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method

Kenichi Miyoshi; Ting Huang; Keiichi Itakura

Nucleic Acids Research | 1980

Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method

Kenichi Miyoshi; Tetsuo Miyake; Toyoharu Hozumi; Keiichi Itakura

Nucleic Acids Research | 1980

Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphotriester method

Kenichi Miyoshi; Rene Arentzen; Ting Huang; Keiichi Itakura

Archive | 1984

[21-leucine] human urogastrone, corresponding gene, corresponding recombinant plasmid, transformed cell and process for production thereof

Kenichi Miyoshi; Shin-ichiro Sumi; Akira Hasegawa; Masanori Suzuki

Archive | 1988

Method for detecting target nucleic acid in specimen

Akio Yamane; Takanori Oka; Satoru Nakagami; Kenichi Miyoshi

Journal of the American Chemical Society | 1981

Solid-phase synthesis of hentriacontanucleotide

Piotr Dembek; Kenichi Miyoshi; Keiichi Itakura

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Collaboration

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Keiichi Itakura

Beckman Research Institute

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Masanori Suzuki

Laboratory of Molecular Biology

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Shin-ichiro Sumi

Yamaguchi University

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Ting Huang

City of Hope National Medical Center

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Akira Hasegawa

Laboratory of Molecular Biology

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Akira Hasegawa

Laboratory of Molecular Biology

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Fusakazu Misoka

Nagasaki University

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Masanori Sugiyama

Hiroshima University

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Takanori Oka

Takata Corporation

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Rene Arentzen

City of Hope National Medical Center

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