Kenio Costa de Lima

Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite.

Given the importance of bacteria in the development of periradicular lesions, the eradication of the root canal infection is paramount in endodontic treatment. This study evaluated the in vitro intracanal bacterial reduction produced by instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite (NaOCl) or saline solution. Root canals inoculated with Enterococcus faecalis were instrumented and irrigated with the solutions tested. Canals were sampled before and after preparation. After serial dilution, samples were plated onto Mitis salivarius agar, and the colony-forming units grown were counted. Inhibitory effects of the three NaOCl solutions on E. faecalis were also evaluated by means of the agar diffusion test. All test solutions significantly reduced the number of bacterial cells in the root canal (p < 0.05). There was no significant difference between the three NaOCl solutions tested (p > 0.05). Nonetheless, all NaOCl solutions were significantly more effective than saline solution in reducing the number of bacterial cells within the root canal (p < 0.05). The three NaOCl concentrations showed large zones of inhibition against E. faecalis. The results of this study suggest that regular exchange and the use of large amounts of irrigant should maintain the antibacterial effectiveness of the NaOCl solution, compensating for the effects of concentration.

Mechanical reduction of the bacterial population in the root canal by three instrumentation techniques

The in vitro reduction of the bacterial population in the root canal by the mechanical action of instrumentation and irrigation was evaluated. Root canals inoculated with a Enterococcus faecalis suspension were instrumented using hand Nitiflex files, Greater Taper (GT) files, and Profile 0.06 taper Series 29 rotary instruments. Irrigation was performed using sterile saline solution. Root canals were sampled before and after instrumentation. In the group of the Nitiflex files, samples were also taken after each file size. After serial dilution, samples were plated onto Mitis-Salivarius agar, and the colony forming units grown were counted. All techniques and instruments tested were able to reduce significantly the number of bacterial cells in the root canal. Instrumentation to a Nitiflex #30 was significantly more effective than GT files. There were no significant differences when comparing the effects of the Profile instrument #5 with either the GT files or the Nitiflex #30. Enlargement to a Nitiflex #40 was significantly more effective in eliminating bacteria when compared with the other techniques and instruments tested (p < 0.05). The results of this study showed that the instrumentation and irrigation can mechanically remove more than 90% of bacterial cells from the root canal.

Efficacy of Instrumentation Techniques and Irrigation Regimens in Reducing the Bacterial Population within Root Canals

The purpose of this study was to compare the in vitro intracanal bacterial reduction produced by using two instrumentation techniques and different irrigation methods. Root canals inoculated with Enterococcus faecalis were prepared by using the following techniques and irrigants: alternated rotary motions (ARM) technique, hand nickel-titanium files and 2.5% sodium hypochlorite (NaOCl) as irrigant; ARM technique and combined irrigation with 2.5% NaOCl and citric acid; ARM technique and combined irrigation with 2.5% NaOCl and 2% chlorhexidine gluconate; and Greater Taper rotary files, using 2.5% NaOCl as irrigant. Controls were instrumented by using the ARM technique and irrigated with sterile saline. Canals were sampled before and after preparation. After serial dilution, samples were plated onto Mitis-Salivarius agar, and the colony forming units that were grown were counted. All test techniques and solutions significantly reduced the number of bacterial cells within the root canal (p < 0.05). There was no significant difference between the experimental groups (p > 0.05). Nonetheless, all of them were significantly more effective than the control group (p < 0.05). These findings support the importance of using antimicrobial irrigants during the chemomechanical preparation, regardless of the solutions or instrumentation techniques used.

Antimicrobial Activity and Flow Rate of Newer and Established Root Canal Sealers

Endodontic sealers that possess both optimum flow ability and antimicrobial properties may theoretically assist in the elimination of microorganisms located in confined areas of the root canal system. The antimicrobial effects and the flow rate of the following sealers were investigated and compared: Kerr Pulp Canal Sealer EWT, Grossmans Sealer, ThermaSeal, Sealer 26, AH Plus, and Sealer Plus. The agar diffusion test was used to assess the antimicrobial activity of the sealers. In the flow assay, the sealers were placed between two glass slabs and a weight of 500 g was placed on the top of the glass. The diameters of the formed discs were recorded. All root canal sealers tested showed some antimicrobial activity against most of the microorganisms. There were no significant differences between the materials tested (p > 0.05). All root canal sealers also flowed under the conditions of this study. Statistical analysis of the results revealed that AH Plus and Kerr Pulp Canal Sealer EWT had flow values significantly superior to the other sealers tested (p > 0.05). Taken together, these findings suggest that these sealers have the potential to help in the microbial control in the root canal system.

Susceptibilities of Enterococcus faecalis Biofilms to Some Antimicrobial Medications

Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulose nitrate membrane filters. Each biofilm-containing membrane was thoroughly covered with 1 ml of the test medications and incubated for 1 day at 37 degrees C. Treated biofilms were then aseptically transferred to vials containing a neutralizing agent in saline solution and vortexed. Suspensions were 10-fold diluted, seeded onto Mitis salivarius agar plates, and the colony-forming units counted after 48 h of incubation. There were significant differences between the formulations tested. The association of clindamycin with metronidazole significantly reduced the number of cells in 1-day biofilms. However of all medications tested, only 2% chlorhexidine-containing medications were able to thoroughly eliminate most of both 1-day and 3-day E. faecalis biofilms.

Inclusion of oral health teams in the Family Health Program and its impact on the use of dental services

The aim of this study was to determine whether the incorporation of Oral Health Teams (OHT) into the Family Health Program (FHP) led to greater use of dental services in Natal, Rio Grande do Norte State, Brazil. Twenty-two census tracts were studied, 11 located in areas covered by the FHP for at least one year and paired according to socioeconomic criteria with 11 tracts in areas without coverage. The final sample consisted of 4,604 subjects. The areas covered by OHT in the FHP (model 1) were compared to areas covered by the FHP but without OHT (model 2) and to areas covered by traditional primary care services or without any coverage (model 3). In areas covered by model 1, 32% of the population used dental care, whereas in models 2 and 3 the rates were 45.4% and 17.5%, respectively (p < 0.001). With respect to age, model 1 only exceeded model 2 in the age bracket 12 years and under. The study concludes that OHT in the FHP only had a positive impact on the target indicator when compared to model 3.

A utilização de serviços odontológicos entre crianças e fatores associados em Sobral, Ceará, Brasil

The aim of this study was to analyze the use of dental services by children in Sobral, Ceará State, Brazil, relating such use to socioeconomic factors and patterns of service consumption. An analytical cross-sectional study was performed, interviewing parents or guardians of 3,425 children. The relationship between independent variables and use of dental services was analyzed using tests of association (chi-squared), odds ratios (OR) and respective confidence intervals, and multiple logistic regression. 50.9% of children had used dental services at least once in their lives. Of these, 65.3% had used such services in the previous year and 85.4% in public dental services. The importance of the Brazilian Unified National Health System is highlighted by the response in terms of access to dental services, considering that the vast majority of treatment was provided by this public system. The factors that most affected use of dental services were socioeconomic status, health plan coverage, owning a toothbrush, regular garbage collection, maternal knowledge, sewage treatment, and malnutrition.

Prevalence and factors associated to actinic cheilitis in beach workers.

OBJECTIVES Assess the prevalence and potential factors associated to actinic cheilitis (AC) in workers exposed to the sun. MATERIALS AND METHODS Workers assigned to urban beaches in a city in Northeastern Brazil completed a questionnaire containing personal and health data. Lips were examined to identify AC. RESULTS A total of 362 workers, of which 15.5% exhibited AC, were examined. Among those with AC, there was a prevalence of males (86%), aged 37years or older (61.4%) and light-skinned (52.6%). They were undocumented (96.5%), exposed to the sun (84.2%), worked up to 6h daily (57.9%), five or more times per week (52.6%), and for more than 8years (54.4%). Photoprotection was used by 89.5%, including sunscreen (42.0%), lip protector (17.5%), and cap/hat (87.7%). Approximately 30% smoked and 29.8% consumed alcohol, 56.2% over two standard shots per day. Most (91.2%) ate healthy food five or more times per week. Being male, aged 37years or older, having up to 6years of schooling, being light-skinned, wearing a cap/hat, and using sunscreen were factors associated to AC. CONCLUSION A high prevalence of actinic AC was observed. Special attention should be given to individuals chronically exposed to UV radiation, with the institution of educational, preventive, and curative measures.

A representação social do cuidado no programa saúde da família na cidade de Natal

This study examines the core and outlying representational elements of healthcare constructed by Family Healthcare Program teams in Natal, Brazil, using the theory of social representations and the central core theory. The sample consisted of ninety healthcare practitioners working with this Program, with data collected through free word association, a questionnaire and focus groups. The core representation consists of attention/love and comfort, disclosing different understandings and showing that accumulated knowledge is supported by a view that is close to care-giving. However, traditional values and trivial connotations are maintained, hindering the implementation of more effective interventions in by this Program. The core composition indicated that any capacity-building efforts that try to modify attitudes - and thus the daily practice of these practitioners - must assign high priority to discussions on redefining these elements of attention/love and comfort. They must consider the set of mental, emotional, and practical elaborations as well as explanations arising from daily life that are introduced into the constitution of the social representations under examination, influencing choices and shaping the strategies used by practitioners to provide care.

Relationship Between Fcγ Receptor and Interleukin-1 Gene Polymorphisms and Post-treatment Apical Periodontitis

INTRODUCTION Genetic polymorphisms have been reported to act as modifiers of diverse diseases and, as such, might theoretically influence the severity and response to treatment of apical periodontitis. The purpose of this study was to investigate the association of Fcgamma receptor and interleukin (IL)-1 gene polymorphisms with post-treatment apical periodontitis in Brazilian individuals. METHODS The study population consisted of 18 patients with post-treatment apical periodontitis and 44 individuals with root canal-treated teeth exhibiting healthy/healing periradicular tissues (controls). Patients were typed for the following genes (alleles): FcgammaRIIA (R131 or H131), FcgammaRIIIB (NA1 or NA2), IL-1A (1 or 2), and IL-1B (1 or 2). RESULTS No significant statistical differences were observed for all specific genotypes and almost all allele carriage rates of the test genes as well as combinations thereof with regard to association with disease (P > .05). Actually, only 2 genetic conditions were found to be associated with post-treatment apical periodontitis: carriage of allele H131 of the FcgammaRIIa gene (P = .04) and a combination of this allele with allele NA2 of the FcgammaRIIIb gene (P < .01). CONCLUSIONS Data from the present study suggest that some conditions associated with polymorphism of Fcgamma receptor genes might influence the patients response to endodontic treatment of teeth with apical periodontitis.