Kit Galvin

Chlorpyrifos exposures in Egyptian cotton field workers

Neurobehavioral deficits have been reported in Egyptian pesticide application teams using organophosphorus (OP) pesticides, but whether these effects are related to OP pesticide exposures has yet to be established. In preparation for a comprehensive study of the relationship between OP pesticide dose and neurobehavioral deficits, we assessed exposure within this population. We conducted occupational surveys and workplace observations, and collected air, dermal patch and biological samples from applicators, technicians and engineers involved in chlorpyrifos applications during cotton production to test the hypotheses that: (1) dermal exposure was an important contributor to internal dose and varied across body regions; and (2) substantial differences would be seen across the three job categories. Applicators were substantially younger and had shorter exposure histories than did technicians and engineers. Applicators and technicians were observed to have relatively high levels of skin or clothing contact with pesticide-treated foliage as they walked through the fields. Both dermal patch loadings of chlorpyrifos and measurements of a chlorpyrifos-specific metabolite (TCPy) in urine confirmed substantial exposure to and skin absorption of chlorpyrifos that varied according to job category; and dermal patch loading was significantly higher on the thighs than on the forearms. These findings support our hypotheses and support the need for research to examine neurobehavioral performance and exposures in this population. More importantly, the exposures reported here are sufficiently high to recommend urgent changes in work practices amongst these workers.

Contributions of inhalation and dermal exposure to chlorpyrifos dose in Egyptian cotton field workers.

Abstract Objective: Chlorpyrifos exposures were assessed in 12 Egyptian cotton field workers. Methods: 3,5,6-trichloro-2-pyridinol (TCPy) was measured in 24-hour urine samples to estimate absorbed dose. Workshift air samples were used to calculate chlorpyrifos inhalation dose. Results: Patches on legs had the highest chlorpyrifos loading rates among body regions sampled. Geometric mean chlorpyrifos air concentrations were 5·1, 8·2, and 45·0 μg/m3 for engineers, technicians, and applicators, respectively; peak TCPy urinary concentrations were 75–129, 78–261, and 487–1659 μg/l, respectively; geometric mean doses were 5·2–5·4, 8·6–9·7, and 50–57 μg/kg, respectively, considering TCPy excretion half-life values of 27 and 41 hours. All worker doses exceeded the acceptable operator exposure level of 1·5 μg/kg/day. An estimated 94–96% of the dose was attributed to dermal exposure, calculated as the difference between total dose and inhalation dose. Discussion: Interventions to reduce dermal exposure are warranted in this population, particularly for the hands, feet, and legs.

Experimental strategy for translational studies of organophosphorus pesticide neurotoxicity based on real-world occupational exposures to chlorpyrifos

Translational research is needed to understand and predict the neurotoxic consequences associated with repeated occupational exposures to organophosphorus pesticides (OPs). In this report, we describe a research strategy for identifying biomarkers of OP neurotoxicity, and we characterize pesticide application workers in Egypts Menoufia Governorate who serve as our anchor human population for developing a parallel animal model with similar exposures and behavioral deficits and for examining the influence of human polymorphisms in cytochrome P450 (CYP) and paraoxonase 1 (PON1) enzymes on OP metabolism and toxicity. This population has previously been shown to have high occupational exposures and to exhibit a broad range of neurobehavioral deficits. In addition to observational studies of work practices in the field, questionnaires on demographics, lifestyle and work practices were administered to 146 Egyptian pesticide application workers applying pesticides to the cotton crop. Survey results indicated that the application workforce uses standard operating procedures and standardized equipment provided by Egypts Ministry of Agriculture, which provides a workforce with a stable work history. We also found that few workers report using personal protective equipment (PPE), which likely contributes to the relatively high exposures reported in these application workers. In summary, this population provides a unique opportunity for identifying biomarkers of OP-induced neurotoxicity associated with occupational exposure.

Longitudinal assessment of occupational exposures to the organophosphorous insecticides chlorpyrifos and profenofos in Egyptian cotton field workers

Chlorpyrifos (CPF) and profenofos (PFF) are organophosphorus (OP) insecticides that are applied seasonally in Egypt to cotton fields. Urinary trichloro-2-pyridinol (TCPy), a specific CPF metabolite, and 4-bromo-2-chlorophenol (BCP), a specific PFF metabolite, are biomarkers of exposure, while inhibition of blood butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities are effect biomarkers that may be associated with neurotoxicity. Urinary TCPy and BCP and blood BChE and AChE activities were measured in 37 adult Egyptian Ministry of Agriculture workers during and after 9-17 consecutive days of CPF application followed by an application of PFF (9-11 days), and a second CPF application (5 days) in 2008. During the OP applications, urinary TCPy and BCP levels were significantly higher than baseline levels, remained elevated following the application periods, and were associated with an exposure related inhibition of blood BChE and AChE. Analysis of blood AChE levels before and after the PFF application period suggests that individual workers with peak BCP levels greater than 1000 μg/g creatinine exhibited further inhibition of blood AChE with PFF application, demonstrating that PFF exposure had a negative impact on AChE activity in this highly exposed worker population. While large interindividual differences in exposure were observed throughout this longitudinal study (peak urinary BCP and peak TCPy levels for individuals ranging from 13.4 to 8052 and 16.4 to 30,107 μg/g creatinine, respectively), these urinary biomarkers were highly correlated within workers (r=0.75, p<0.001). This suggests that the relative exposures to CPF and PFF were highly correlated for a given worker. The variable exposures between job classification and work site suggest that job title and work location should not be used as the sole basis for categorizing OP exposures when assessing neurobehavioral and other health outcomes in Egyptian cotton field workers. Together, these findings will be important in educating the Egyptian insecticide application workers in order to encourage the development and implementation of work practices and personal protective equipment to reduce their exposure to CPF and PFF.

Organophosphorus pesticide exposure and neurobehavioral performance in Latino children living in an orchard community

Children living in agricultural communities have a greater risk from pesticides due to para-occupational pathways. The goal of this study was to assess the impact of exposure to organophosphorus pesticides on the neurobehavioral performance of school-aged Latino children over time. Two exposure measures were used to estimate childrens pesticide exposure: parents occupation (agricultural or non-agricultural) and organophosphate residues in home carpet dust samples. During 2008-2011, 206 school-aged children completed a battery of neurobehavioral tests two times, approximately one year apart. The associations between both exposure measures and neurobehavioral performance were examined. Pesticide residues were detected in dust samples from both agricultural and non-agricultural homes, however, pesticides were detected more frequently and in higher concentrations in agricultural homes compared to non-agricultural homes. Although few differences were found between agricultural and non-agricultural children at both visits, deficits in learning from the first visit to the second visit, or less improvement, was found in agricultural children relative to non-agricultural children. These differences were significant for the Divided Attention and Purdue Pegboard tests. These findings are consistent with previous research showing deficits in motor function. A summary measure of organophosphate residues was not associated with neurobehavioral performance. Results from this study indicate that children in agricultural communities are at increased risk from pesticides as a result of a parent working in agricultural. Our findings suggest that organophosphate exposure may be associated with deficits in learning on neurobehavioral performance, particularly in tests of with motor function. In spite of regulatory phasing out of organophosphates in the U.S., we still see elevated levels and higher detection rates of several organophosphates in agricultural households than non-agricultural households, albeit lower levels than prior studies.

Practical Solutions for Pesticide Safety: A Farm and Research Team Participatory Model

ABSTRACT Development of the Practical Solutions for Pesticide Safety guide used participatory research strategies to identify and evaluate solutions that reduce pesticide exposures for workers and their families and to disseminate these solutions. Project principles were (1) workplace chemicals belong in the workplace, and (2) pesticide handlers and farm managers are experts, with direct knowledge of production practices. The project’s participatory methods were grounded in self-determination theory. Practical solutions were identified and evaluated based on five criteria: practicality, adaptability, health and safety, novelty, and regulatory compliance. Research activities that had more personal contact provided better outcomes. The Expert Working Group, composed of farm managers and pesticide handlers, was key to the identification of solutions, as were farm site visits. Audience participation, hands-on testing, and orchard field trials were particularly effective in the evaluation of potential solutions. Small work groups in a Regional Advisory Committee provided the best direction and guidance for a “user-friendly” translational document that provided evidence-based practical solutions. The “farmer to farmer” format of the guide was endorsed by both the Expert Working Group and the Regional Advisory Committee. Managers and pesticide handlers wanted to share their solutions in order to “help others stay safe,” and they appreciated attribution in the guide. The guide is now being used in educational programs across the region. The fundamental concept that farmers and farmworkers are innovators and experts in agricultural production was affirmed by this study. The success of this process demonstrates the value of participatory industrial hygiene in agriculture.

Organophosphorus pesticide residue levels in homes located near orchards

Abstract Organophosphorus pesticides (OPs) are commonly applied to agricultural crops. Families living in these communities may have higher exposure to OPs due to take home exposures and close proximity to agricultural fields. The objectives of this study were to measure OP concentrations in home carpet dust in agricultural and non-agricultural households and examine factors that may impact OP concentrations such as occupation, housing characteristics, and resident behaviors. Agricultural households had at least one parent who worked in agriculture during the previous 5 years. Carpet dust samples were collected at two time points from 278 households in an agricultural community located in the Pacific Northwest from 2008–2011. Samples were analyzed for four types of OPs: azinphos-methyl, phosmet, malathion, and chlorpyrifos. Overall, OP detection frequencies and concentrations were higher in agricultural households compared to non-agricultural households. Factors associated with higher OP concentrations in home carpet dust were identified and included: (1) homes with two or more agricultural workers living in the home, (2) homes located in close proximity to an agricultural field or orchard, (3) having an entry floor mat, and (4) frequently vacuuming the house. Having air conditioning in the home had a protective effect with OP concentrations. While the use of these four OPs is restricted or limited for residential use in the United States, results show that they were still found in the indoor environment. The understanding of the impact of agricultural work and other factors that elevate levels of OPs in the home is crucial to mitigating pesticide exposure in agricultural communities.

Practical Solutions for Pesticide Safety: Translating and Disseminating Research, Evaluating Impact

and website. The media campaign, “Parent First, Farmer Second,” was designed to motivate by acknowledging the many important roles that farmers have while reminding them that the most important role is keeping their children safe. The website (cultivatesafety.org) was developed to be a parent-friendly site housing easy-to-use information on child development and best safety practices. The 5-month pilot media campaign began March 2013 in Wisconsin with radio ads, print ads in agricultural newspapers/journals, and Internet ads on agricultural news sites directing traffic to the website. Website analytics and uploads are monitoring “actions,” which include: 1) visitor traffic based on the timing and type of ad; 2) website referrals; and 3) website utilization (visit time, page views, downloads and testimonial uploads). Over the 5-month pilot media campaign, 12 print ads, 120 radio ads, and 25,000–50,000 banner ads/month on agricultural news sites featured the campaign. The website averaged 10 visits per day and the visits did not appear to be influenced by the timing of ads. The site received 50% direct traffic from print and radio ads, 38% traffic from referral websites such as www.agriculture.com and 12% traffic from keyword searches. Website utilization was characterized with a 0-10 second engagement by 67% of the visitors and a 63% bounce rate (calculated when visitors leave the site rather than viewing other pages). Overall, the media campaign appeared to have only a small effect on website visits and utilization. With this project, we bridged research findings on the efficacy of childhood farm injury bestpractice guidelines with practical strategies to get easy-to-use information into the hands of farmers.

Comparison of Exposure to Repetitive Upper Arm Motions and Non-neutral Upper Arm Postures between Apple Harvesting with Ladders and Mobile Platforms

In attempts to improve productivity in agriculture, a harvest-assisting mobile platform has been developed and is being evaluated for use in Washington State apple orchards; however, its ergonomic effect on work postures and repetitive motions has not yet been characterized. The purposes of this study were: 1) to compare physical exposures between two methods of apple harvesting: a conventional ladder and a newly-developed mobile platform, and 2) to develop systematic methods for characterizing work repetitions, specifically in the upper arms. Using self-contained tri-axial inclinometers with built-in memory, upper arm inclinations were continuously monitored from twenty male workers over a full day of apple harvesting. At the beginning and the end of work shift, Borg CR10 scales were used as self-report measures of perceived fatigue in the shoulder. Postural exposures were characterized as the percent of time the upper arm postures exceeded specified angle ranges. Repetition was evaluated using changes in upper arm angles ranging from 5 to 30 degrees. In terms of prolonged postural exposures, repetitive work exposure and perceived fatigue in the shoulders, the workers using the mobile platform had less physical stress compared to the workers using the ladders. In addition, the upper arm repetition rates were high (over 15 repetitions per minute) for both harvesting methods. A more systematic study of the harvesting methods is needed to characterize differences in repetition rates and productivity between the mobile platforms and ladders. However, the results indicate that the mobile platform could improve workability and may enable more diverse workers to participate in harvesting activities.