Kobun Rovina

Heavy Metal Contaminants Removal from Wastewater Using the PotentialFilamentous Fungi Biomass: A Review

Heavy metal pollution of wastewater currently becomes a key environmental problem throughout the whole world. Conventional methods for the removal of heavy metals from aqueous solutions are not economically and environmental friendly because it has produced massive quantity of toxic chemical compounds. Recently, the removal of heavy metals from wastewater are extensively used various conventional methods such as chemical precipitation, coagulation-flocculation, flotation, ion exchange and membrane filtration. Biological treatments, especially filamentous fungi have gained an increasing attention for heavy metal removal and recovery due to their upright performances, low cost and huge quantities. The filamentous fungi have a great potential to produce large amount of biomasses which are widely used for metal adsorption capacities of Pb, Zn, Cd, Cu, Cr, As and Ni. Production of biomass has offered great potential for adopting metal-recovery system. The main aim of this review paper is to discuss the available information of heavy metals removal for the utilization of filamentous fungi biomass and scrutinize the practical of exploiting them for heavy metal remediation.

Extraction, Analytical and Advanced Methods for Detection of Allura Red AC (E129) in Food and Beverages Products.

Allura Red AC (E129) is an azo dye that widely used in drinks, juices, bakery, meat, and sweets products. High consumption of Allura Red has claimed an adverse effects of human health including allergies, food intolerance, cancer, multiple sclerosis, attention deficit hyperactivity disorder, brain damage, nausea, cardiac disease and asthma due to the reaction of aromatic azo compounds (R = R′ = aromatic). Several countries have banned and strictly controlled the uses of Allura Red in food and beverage products. This review paper is critically summarized on the available analytical and advanced methods for determination of Allura Red and also concisely discussed on the acceptable daily intake, toxicology and extraction methods.

A Review of Peptide Nucleic Acid

Peptide Nucleic Acid (PNA) is a nucleobase oligomer in which the whole backbone is mainly replaced by N-(2- aminoethyl) glycine units. PNA is considered as DNA with a neutral peptide backbone due to negative charged sugar–phosphate backbone. It is chemically stable and resistant to hydrolytic cleavage. PNA can be categorized specific sequences of DNA and RNA according to Watson–Crick hydrogen bonding structure. Hybridization process showed high thermal stability and unique ionic strength effects. It is formed a stable PNA/DNA/PNA triplex with a looped-out DNA strand. PNA hybridization technology is promptly developed within in situ hybridization. In our review paper was elaborated the PNA superior hybridization characteristics, importance’s of PNA and major applications of PNA in the diagnostic and pharmaceutical fields. And also PNA could be replaced DNA in uses as a probe for many investigation purposes. PNAs antisense activities have found in nerve cells and even in rats upon injection into the brain, and in Escherichia coli.

A Review of Extraction and Analytical Methods for the Determination of Tartrazine (E 102) in Foodstuffs

ABSTRACT Tartrazine is an azo food dye, which is orange-colored and water soluble. It is usually used in foods, pharmaceuticals, cosmetics, and textiles. Tartrazine has the potential to cause an adverse health effect on humans, such as hyperactivity in children, allergy, and asthma. Joint FAO/WHO Expert Committee on Food Additive and EU Scientific Committee for Food have standardized the acceptable daily intake for tartrazine that is 7.5 mg kg−1 body weight. Many researchers have detected the presence of tartrazine for monitoring the quality and safety of food products. In this review paper, we highlighted various tartrazine detection and extraction methods. Some of the analytical methods are available such as high-performance liquid chromatography, electrochemical sensor, thin-layer chromatography, spectrophotometry, capillary electrophoresis, and liquid chromatography-tandem mass spectrometry. Also, we discuss following extraction steps: liquid–liquid extraction, solid-phase extraction, membrane filtration, cloud point extraction, and other extraction method. In addition, a brief overview is presented explaining the synthesis process and metabolism of tartrazine and the maximum permitted level in different countries. This review paper will give an insight into different extraction and analytical methods for the determination of tartrazine in healthy foods, which will attract the attention of public toward food safety and quality, and also the interest of food industry and government bodies.

Highly sensitive determination of sunset yellow FCF (E110) in food products based on Chitosan/Nanoparticles/MWCNTs with modified gold electrode

Sunset Yellow belongs to the family of azo dyes, commonly used in food industry. High consumption of Sunset Yellow can cause health problem to human. Due to arising of the health issues, there are several analytical methods available for determination of Sunset Yellow. However, these methods are required skilled manpower, complicated procedures, time consuming and high cost. Herein, an electrochemical sensor was developed based on the combination of chitosan (CHIT), calcium oxide nanoparticles (CaONPs) and multiwall carbon nanotubes (MWCNTs) sensing film for detection of Sunset Yellow in food products. Electrochemical behavior of the modified gold electrode in the presence of Sunset Yellow was studied by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The morphological characteristics of CHIT/CaONPs/MWCNTs were observed under scanning electron microscope and transmission electron microscope. Under optimal conditions, the DPV was detected with different concentrations of Sunset Yellow in the range of 0.9 to 10 ppm, with detection limit of 0.8 ppm. The developed method has successfully applied for monitoring the presence of Sunset Yellow with different food products including candy, royal jelly, ice cream and soft drink with satisfactory results.

Analytical and advanced methods-based determination of melamine in food products

Abstract Melamine is nitrogen-rich (66%) chemical that has currently received more attention due to highly publicized domestic and international food safety incidents. The contamination of pet food caused hundreds of pet deaths in North America during 2007. In Sep. 2008, it became a big issue in China when 6 infants died and more than 300,000 people were hospitalized due to melamine contamination of milk powder. Melamine is added in foods purposely to elevate the actual protein content in order to achieve a better price and gain high profit from the products. Recently, several methods have been established for determination of melamine. Unfortunately, most of the methods require complicated preconcentration, time-consuming methods, and costly instruments. However, there are increasing demands to develop a new, fast, simple, convenient, and sensitive method for determination of melamine. The use of simpler and faster analytical procedures based on sensors has emerged in scientific literature as a very promising alternative method. This chapter discusses the current state of advances detection techniques in melamine and its compounds. Other sections deal with current and past melamine contamination incidents, together with the modern instrumental analytical methods for determining the presence of melamine and its analogs.