Kumasaka T

Time of ovulation and prolonged pregnancy

Abstract In a prospective study of 110 women who were delivered of live infants following the spontaneous onset of labor, the time was calculated between delivery and (1) the first day of the last menstrual period, and (2) the day on which ovulation had probably taken place. The results of basal body temperature recordings were used to estimate the day of ovulation. Statistical analysis showed that delay of ovulation was the major contributing cause of apparent prolongation of pregnancy over 295 days.

Effect of Postovulatory Treatment with a Luteinizing Hormone-Releasing Hormone Analog on the Plasma Level of Progesterone in Women

(des-Gly-NH2(10),Pro-ethylamide9) luteinizing hormone-releasing hormone (LH-RH) (100 microgram) was administered subcutaneously once daily for 5 days during the postovulatory period in six women with regular menstrual cycles. Plasma levels of luteinizing hormone, follicle-stimulating hormone, estradiol, and progesterone were measured daily by radioimmunoassay from the ovulatory stage to menses. Suppression of plasma progesterone occurred during the treated luteal phase as compared with that of the control luteal phase. This finding suggests that repetitive, massive, endogenous luteinizing hormone or massive, exogenous, LH-RH itself during the early luteal phase of the cycle may induce functional luteolysis.

Demonstration of immunoreactive somatostatin-like substance in villi and decidua in early pregnancy☆

With the use of the radioimmunoassay for growth hormone--releasing inhibiting factor (GIF), it was found that measurable amounts of GIF-like substance existed in the chorionic villi and decidua of pregnant women. The indirect immunofluorescent method revealed that the higher intensity of GIF-like immunofluorescence was presented in cytotrophoblasts rather than in syncytiotrophoblasts of the villi and in stromal cells of the decidua.

Site of Ovulation and Ectopic Pregnancy

Abstract. In a study of 130 tubal pregnancies the relationships as to location of the corpus luteum, the implantation site, and the gross findings of the non‐pregnant fallopian tube were analyzed. A contralateral corpus luteum was found in 20% of the cases. Hydrosalpinx, peritubal adhesions and/or thickening of the tubal wall were observed in 33% of the non‐pregnant tubes. Grouping by implantation site—ipsilateral or contralateral corpus luteum—showed no statistical differences. The incidence of chronic pelvic inflammatory diseases was no greater in the subjects above 30 years of age and those having had more than 2 pregnancies. The results suggest that “tube locking” of the ovum, sometimes a result of previous tubal inflammatory disease, sometimes a result of supposed insufficiency of tubal peristalsis. was the major cause of tubal gestation. External migration of the ovum alone may not be an important factor in the genesis of tubal pregnancy.

The prolactin-releasing mechanism of the hypothalamo-pituitary axis in pregnancy**Presented at the Thirty-Third Annual Meeting of the Japan Society of Obstetrics and Gynecology, May 11, 1981, Niigata, Japan.

The purpose of this study was to investigate the prolactin (PRL)-releasing mechanism of the hypothalamo-pituitary axis in pregnancy. Forty-six gravidas in the first and second trimesters received bromocriptine (BRC), 2.5 mg orally, metoclopramide (MCP), 10 mg intravenously, or thyrotropin-releasing hormone (TRH), 500 micrograms intravenously. Additionally, BRC was given orally to another 42 gravidas 60 minutes prior to the intravenous injection of MCP or TRH. The plasma PRL levels decreased significantly after BRC and remained significantly elevated after MCP or TRH administration. However, there were no significant differences of PRL response to these agents between the first- and second-trimester groups. The PRL release from the pituitary by MCP or TRH was suppressed significantly by pretreatment of BRC in the gravidas. We concluded that the control mechanism of PRL secretion remained unchanged in the first and second trimesters.

The Immunoreactive-GIF Concentrations in Various Fetal Tissues Measured by Two Different RIA Systems

Since the discovery of the structures of somatostatin (GIF) in 1973 by Brazeau et al, its measurement by the radioimmunoassay (RIA) methods has been reported by Arimura et al (1975), Yanaihara et al (1978) and Sawano et al (1978). As GIF does not contain tyrosine and histidine, which can be radioiodized, analogues of GIF are being used as tracers in its radioimmunoassay. In this study, two different types of tracers(1251-tyr8-GIF and 125I-tyrosyl-GIF) were used in RIA to measure the immunoreactive GIF of fetal tissues, and their results were compared.

VAGINAL REPAIR IN THE RADICAL OPERATION FOR CERVICAL CARCINOMA

An attempt was made to preserve postoperative sexual function and the recovery of bladder function by a simple modification of technique in 22 cases of radical operations for invasive carcinoma of the cervix. The procedures consisted of two parts. After removal of the specimen, the bladder peritoneal flap is sutured to the anterior vaginal wall leaving a 2 to 3 cm margin between the line of suture and the edge of the peritoneum. Similarly, the posterior peritoneal flap is sutured to posterior vaginal wall leaving a margin. Both edges of the peritoneum are then closed forming a pouch as an extension of vaginal canal. The length of the vagina was successfully elongated by this procedure, thus adding to better postoperative sexual function.

Der Effekt von synthetischem LH-RH auf Serum LH, FSH und Oestrogene, Pregnandiol bzw. Pregnantriol im Urin an verschiedenen Phasen des normalen menstruellen Cyclus

4. Herren M. Saito, T. Kumasaka, ~. I~/iehi, K. Kato, Y. Yaoi, T. Koyama und T. 0hkura (Department of Obstetrics and Gynecology, Tokyo Medical and Dental University Tokyo, Japan) und A. Arimura (School of Medicine, Tulane University New Orleans, La., U.S.A.): Der Effekt yon synthetisehem LH-RtI auf Serum LH, FSH und 0estrogene, Pregnandiol bzw. Pregnantriol im Urin an versehiedenen Phasen des normalen menstruellen Cyelus.