Nozomu Nishi

Time of ovulation and prolonged pregnancy

Abstract In a prospective study of 110 women who were delivered of live infants following the spontaneous onset of labor, the time was calculated between delivery and (1) the first day of the last menstrual period, and (2) the day on which ovulation had probably taken place. The results of basal body temperature recordings were used to estimate the day of ovulation. Statistical analysis showed that delay of ovulation was the major contributing cause of apparent prolongation of pregnancy over 295 days.

Demonstration of immunoreactive somatostatin-like substance in villi and decidua in early pregnancy☆

With the use of the radioimmunoassay for growth hormone--releasing inhibiting factor (GIF), it was found that measurable amounts of GIF-like substance existed in the chorionic villi and decidua of pregnant women. The indirect immunofluorescent method revealed that the higher intensity of GIF-like immunofluorescence was presented in cytotrophoblasts rather than in syncytiotrophoblasts of the villi and in stromal cells of the decidua.

Site of Ovulation and Ectopic Pregnancy

Abstract. In a study of 130 tubal pregnancies the relationships as to location of the corpus luteum, the implantation site, and the gross findings of the non‐pregnant fallopian tube were analyzed. A contralateral corpus luteum was found in 20% of the cases. Hydrosalpinx, peritubal adhesions and/or thickening of the tubal wall were observed in 33% of the non‐pregnant tubes. Grouping by implantation site—ipsilateral or contralateral corpus luteum—showed no statistical differences. The incidence of chronic pelvic inflammatory diseases was no greater in the subjects above 30 years of age and those having had more than 2 pregnancies. The results suggest that “tube locking” of the ovum, sometimes a result of previous tubal inflammatory disease, sometimes a result of supposed insufficiency of tubal peristalsis. was the major cause of tubal gestation. External migration of the ovum alone may not be an important factor in the genesis of tubal pregnancy.

Effect of induced hyperprolactinemia on the plasma levels of C19 steroids in humans

In order to investigate the relationship between the increment of plasma prolactin (PRL) levels and the change of plasma levels of PRL, DHEA-S, cortisol, aldosterone and 17 alpha OH delta 5-P were quantified by respective RIA in patients treated with TRH parenterally or with sulpiride orally. The results were as follows: During the infusion of TRH, plasma levels of PRL reached the maximal values within 30 min and then declined gradually in spite of continued TRH stimulation. Sulpiride given orally for 12 consecutive days in the luteal phase of the menstrual cycle caused a significant increase in the plasma PRL level. This increase was higher than that obtained in the experiment during the follicular phase. No significant change in the plasma level of 17 alpha OH delta 5-P was observed TRH sulpiride administration. In 3 out of 9 cases, plasma DHEA-S levels were elevated significantly on day 13 to 14 of the experimental course. These results indicate that the responsiveness of plasma levels of adrenal androgens to the raised plasma PRL level requires a certain period of time in the hyperprolactinemic state in the human female.

The Immunoreactive-GIF Concentrations in Various Fetal Tissues Measured by Two Different RIA Systems

Since the discovery of the structures of somatostatin (GIF) in 1973 by Brazeau et al, its measurement by the radioimmunoassay (RIA) methods has been reported by Arimura et al (1975), Yanaihara et al (1978) and Sawano et al (1978). As GIF does not contain tyrosine and histidine, which can be radioiodized, analogues of GIF are being used as tracers in its radioimmunoassay. In this study, two different types of tracers(1251-tyr8-GIF and 125I-tyrosyl-GIF) were used in RIA to measure the immunoreactive GIF of fetal tissues, and their results were compared.