Kyong-Ho Kim

Phytoremediation of heavy metals in contaminated water and soil using Miscanthus sp. Goedae-Uksae 1.

The aim of this study is to characterize the heavy metal phytoremediation potential of Miscanthus sp. Goedae-Uksae 1, a hybrid, perennial, bio-energy crop developed in South Korea. Six different metals (As, Cu, Pb, Ni, Cd, and Zn) were used for the study. The hybrid grass effectively absorbed all the metals from contaminated soil. The maximum removal was observed for As (97.7%), and minimum removal was observed for Zn (42.9%). Similarly, Goedae-Uksae 1 absorbed all the metals from contaminated water except As. Cd, Pb, and Zn were completely (100%) removed from contaminated water samples. Generally, the concentration of metals in roots was several folds higher than in shoots. Initial concentration of metals highly influenced the phytoremediation rate. The results of the bioconcentration factor, translocation factor, and enrichment coefficient tests indicate that Goedae-Uksae 1 could be used for phytoremediation in a marginally contaminated ecosystem.

Effect of Film Layers and Storing Period on the Fermentation Quality of Whole Crop Barley Silage

Dept. Animal bio, Chanbuk National University, Jeonju, 561-756ABSTRACTThis study was undertaken to characterize feed value and silage quality according to storage period and film layers for whole-crop barley silage. The crude protein(CP) content increased in all silage during the storage periods compared to those before silage,this content slightly increased over the prolonged storage period but it was not significant(p>0.05). Depending on the film layers of silage, 6 layers were higher than 4 layers. The neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents also increased in all silage during storage periods compared to those before silage(P<0.05), but they were maintained at similar levels during the storage period. Depending on the film layers of silage, 6 layers were higher than 4 layers. The total digestible nutrients(TDN) content decreased in all silage during the storage periods. However, it was maintained at a similar level for the duration of each storage period. Depending on the film layers of silage, 6 layers were lower than 4 layers. The pH value was decreased during the prolonged storage period and depending on the film layers, 6 layers were lower than 4 layers.In the organic acid contents during the prolonged storage period, lactic acid increased, acetic acid was lower, and butyric acid was significantly higher(p<0.05). Depending on the film layers, 6 layers showed higher levels of lactic acid and lower levels of butyric acid(p<0.05). Therefore, these results showed that 6 layer wrapping was advantageous for long term storage of whole crop barley silage, while also indicating that it is desirable to use 4 layer wrapping within a six month period.(Key words :Whole crop barley, Storing period, Film layers, Fermentation quality)

Comparative Pathogenicity of Fusarium graminearum Isolates from Wheat Kernels in Korea

Fusarium head blight (FHB) caused by Fusarium species is a major disease of wheat and barley around the world. FHB causes yield reductions and contamination of grains with trichothecene mycotoxins including; nivalenol (NIV), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), and 15-acetylde-oxynivalenol (15-ADON). The objectives of this study were to identify strains of F. graminearum isolated in Korea from 2012-harvested wheat grain and to test the pathogenicity of these NIV- and DON-producing isolates. Three hundred and four samples of wheat grain, harvested in 2012 in Chungnam, Chungbuk, Gyeongnam, Jeonbuk, Jeonnam, and Gangwon provinces were collected. We recovered 44 isolates from the 304 samples, based on the PCR amplification of internal transcribed spacer (ITS) rRNA region and sequencing. Our findings indicate that F. asiaticum was the predominant (95% of all isolates) species in Korea. We recovered both F. asiaticum and F. graminearum from samples collected in Chungnam province. Of the 44 isolates recovered, 36 isolates had a NIV genotype while 8 isolates belonged to the DON genotype (3-ADON and 15-ADON). In order to characterize the pathogenicity of the strains collected, disease severity was assessed visually on various greenhouse-grown wheat cultivars inoculated using both NIV- and DON-producing isolates. Our results suggest that Korean F. graminearum isolates from wheat belong to F. asiaticum producing NIV, and both F. graminearum and F. asiaticum are not significantly different on virulence in wheat cultivars.

Covered Barley(Hordeum vulgare L.) Variety, ‘Hyeyang’ with BaYMV Resistance, High Yield and Good Malt Quality

Improvement of winter hardiness, lodging resistance and good quality have been recently received more attention by covered barley(Hordeum vulgare L.) breeders than ever in Korea. ‘Hyeyang’, a new covered barley cultivar with early maturing and high yield was developed by the National Institute of Crop Science, RDA in 2010. It was derived from the cross between‘Milyang87’and‘Wolsung8120’with good quality. The initial cross was done in 1999 and the selected line, ‘Iksan432’ (YB4494-B-B-21-2), showed high yield and good quality characteristics under yield trial test from 2008 to 2010. The following is the characteristics of‘Hyeyang’that is characterized as the vernalization of I, green leaf, compact spike and long rough awns. The heading date of‘Hyeyang’were similar to‘Olbori’. The culm length was 82 cm which was 6 cm shorter than‘Olbori’. It showed the spike length of 4.3 cm, 798 spikes / m 2 , 56 grains / spike and 26.7 g for 1,000 grains weight. It showed similar winter hardiness and stronger resistance to barley yellow mosaic virus (BaYMV) compare to‘Olbori’. It showed higher diastatic power 235DP than that of check cultivar, ‘Olbori’. Average yield of ‘Hyeyang’ was 4.44 MT/ha in the regional yield trials carried out in four location at double cropping system in Korea from 2008 to 2010, which was 8 percent higher than ‘Olbori’. ‘Hyeyang’would be suitable for the area above –8°C of daily minimum temperature in January in Korean peninsula.