Kyong-Suk Jin

Betulinic acid isolated from Vitis amurensis root inhibits 3-isobutyl-1-methylxanthine induced melanogenesis via the regulation of MEK/ERK and PI3K/Akt pathways in B16F10 cells.

Previously, betulinic acid was identified as one of the main compounds responsible for the anti-melanogenic effect in Vitis amurensis root. In this study, we investigated the precise mechanism underlying the anti-melanogenic activity of betulinic acid in B16F10 cells. Betulinic acid significantly attenuated 3-isobutyl-1-methylxanthine (IBMX)-induced melanin production by inhibiting tyrosinase, tyrosinase related protein (TRP)-1, and TRP-2 expression through the modulation of their corresponding transcription factors, microphthalamia associated transcription factor (MITF) and cAMP response element binding protein (CREB), in B16F10 cells. In addition, phosphorylation of mitogen-activated protein kinase kinase (MEK)/extracellular regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K)/Akt, involved in the melanogenic processes, were ameliorated by betulinic acid treatment. Role of MEK/ERK and PI3K/Akt signaling pathway in the melanogenesis was confirmed by using specific inhibitors, PD98059 (for MEK/ERK) and LY294002 (for PI3K/Akt), respectively. As a result, betulinic acid inhibited melanin production by tyrosinase, TRP-1, and TRP-2 inhibition through the regulation of CREB and MITF, which was accompanied with MEK/ERK and PI3K/Akt inactivation in IBMX-stimulated B16F10 cells. Consequently, these results demonstrate a novel molecular function of betulinic acid derived from V. amurensis root in melanogenesis, which in turn enhances our understanding on the application of cosmetic therapy for reducing skin hyperpigmentation.

Antiadipogenic Effect of Vitis amurensis Root Methanol Extract and Its Solvent Fractions in 3T3-L1 Preadipocytes

왕머루(Vitis amurensis Rupreche)는 아시아 지역에서 자생하는 포도의 한 종류로서 항산화, 항염증, 뇌신경보호, 신생혈관 억제능 등의 생리활성이 알려져 있다. 왕머루의 뿌리는 한국에서 전통적인 약재로 사용되어왔으나 그 생리활성에 대해서는 보고된 바가 적다. 이에 본 연구에서는 머루근 MeOH 추출물(VARM)과 그 용매 분획물이 지방세포분화 및 지방 생성에 미치는 영향을 3T3-L1 preadipocyte를 이용하여 분석하였다. 그 결과 VARM은 3T3-L1 preadipocyte의 지방 세포로의 분화, 지방 축적, 중성 지방 생성량을 독성 없이 농도의존적으로 유의적으로 억제시켰다. 활성 물질의 규명을 위해 VARM으로부터 dichloromethane (

Antiobesity Activity of Chrysanthemum zawadskii Methanol Extract

CH_2Cl_2

Juniperus chinensis and the functional compounds, cedrol and widdrol, ameliorate α-melanocyte stimulating hormone-induced melanin formation in B16F10 cells

), ethyl acetate (EtOAc), n-butanol (n-BuOH), 그리고 물(

Vitis amurensis Ruprecht root inhibited α-melanocyte stimulating hormone-induced melanogenesis in B16F10 cells

H_2O

Antioxidative and Anti-inflammatory Activities of Ardisia arborescens Ethanol Extract

) 층의 순서로 용매 분획을 실시한 후 지방 생성에 미치는 영향을 분석한 결과

Anti-oxidative and Anti-inflammatory Activities of Decaisnea insignis Ethanol Extract

H_2O

Anti-Oxidative, Anti-Inflammatory, and Anti-Melanogenic Activities of Endlicheria Anomala Extract

층을 제외한 모든 분획물에서 지방 생성 억제능이 관찰되었으며 특히

Induction of G2/M Arrest and Apoptosis by the Methanol Extract of Typha orientalis in Human Colon Adenocarcinoma HT29 Cells

CH_2Cl_2

Anti-Oxidant, Anti-Melanogenic, and Anti-Inflammatory Activities of Zanthoxylum schinifolium Extract and its Solvent Fractions

층 분획물이 가장 강력한 항비만 활성을 보였고 이는 주요 지방 생성 전사 인자인