Jung Ae Park

In vitro Inhibitory Effect of Aged Black Garlic Extract with Antioxidant Activity on MMP-2 and MMP-9 Related to Metastasis

The oxidative damage of lipids, protein, and DNA is known to be involved in not only chronic inflammations such as arthritis, hepatitis, nephritis, gastritis, colitis, and periodontitis but also metastasis. It has given impetus to searching for natural compounds without toxicity, which prevent the development of these diseases. The direct scavenging effects of aged black garlic extract (ABGE) were evaluated in vitro on DPPH radical, hydroxyl radical, hydrogen peroxide, and genomic DNA damage related to oxidative stress. Furthermore, its antioxidant effect on lipid peroxidation was investigated in human fibrosarcoma cells (HT1080), which were exposed to the hydroxyl radical generated by the Fenton reaction. It was observed that ABGE exhibited a greater inhibitory effect on hydrogen peroxide than other reactive oxygen species, and also blocked DNA oxidation and lipid peroxidation induced by the hydroxyl radical. The oxidative stress in live cells was also inhibited in the presence of ABGE. In addition, its inhibitory effects on the activity and expression of MMP-2 and -9 related to metastasis were determined using gelatin zymography and western blot. The data showed that it inhibited MMP-2 and -9 in PMA-stimulated HT1080 cells. Therefore, these results suggest that ABGE show potential as an excellent agent for prevention of metastasis related to oxidative stress.

Antiadipogenic Effect of Vitis amurensis Root Methanol Extract and Its Solvent Fractions in 3T3-L1 Preadipocytes

왕머루(Vitis amurensis Rupreche)는 아시아 지역에서 자생하는 포도의 한 종류로서 항산화, 항염증, 뇌신경보호, 신생혈관 억제능 등의 생리활성이 알려져 있다. 왕머루의 뿌리는 한국에서 전통적인 약재로 사용되어왔으나 그 생리활성에 대해서는 보고된 바가 적다. 이에 본 연구에서는 머루근 MeOH 추출물(VARM)과 그 용매 분획물이 지방세포분화 및 지방 생성에 미치는 영향을 3T3-L1 preadipocyte를 이용하여 분석하였다. 그 결과 VARM은 3T3-L1 preadipocyte의 지방 세포로의 분화, 지방 축적, 중성 지방 생성량을 독성 없이 농도의존적으로 유의적으로 억제시켰다. 활성 물질의 규명을 위해 VARM으로부터 dichloromethane (

Antiobesity Activity of Chrysanthemum zawadskii Methanol Extract

CH_2Cl_2

Inhibitory Effect of Aged Black Platycodi Radix Extract on Expression and Activation of Matrix Metalloproteinases in Oxidative-stressed Melanoma Cells

), ethyl acetate (EtOAc), n-butanol (n-BuOH), 그리고 물(

Anti-Oxidant, Anti-Melanogenic, and Anti-Inflammatory Activities of Zanthoxylum schinifolium Extract and its Solvent Fractions

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The Anti-Obesity Effect of Smilax china Extract

) 층의 순서로 용매 분획을 실시한 후 지방 생성에 미치는 영향을 분석한 결과

Anti-Oxidative and Anti-Obesity Activities of Tetrapanax papyriferus and Siegesbeckia pubescens Extracts and their Synergistic Anti-Obesity Effects

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Anti-Oxidative and Anti-Obesity Effects of Amomum Cardamomum L. Extract

층을 제외한 모든 분획물에서 지방 생성 억제능이 관찰되었으며 특히

머루근 추출물 및 분획물의 항비만 활성

CH_2Cl_2

Anti-Obesity Activity of Euptelea Pleiosperma Ethanol Extract

층 분획물이 가장 강력한 항비만 활성을 보였고 이는 주요 지방 생성 전사 인자인