L. De Stefano

Marine Diatoms as Optical Biosensors

We have chemically modified the frustules of the marine diatom Coscinodiscus concinnus Wm. Smith to properly bind a highly selective bioprobe such as an antibody. By measuring the changes in the photoluminescence emission of diatoms frustules, we have monitored the molecular recognition event between the antibody and its ligand: the dissociation constant estimated is of the same order of that measured by standard Biacore. The nanostructured silica frustules, a low-cost and natural available material, have shown high sensitivity, equal to 1.2+/-0.2 nm microM(-1), and a detection limit of 100 nM, and thus are quite ideal candidates for lab-on-particle applications.

Highly sensitive optochemical gas detection by luminescent marine diatoms

The modifications of photoluminescence properties of silica frustules of different marine diatoms induced by adsorption of nitrogen dioxide, methane, and carbon monoxide are reported. Different species of diatoms were found to exhibit different relative responses and different gas concentration ranges of sensitivity, depending on the morphology and porosity of their frustules. The photoluminescence quenching shows surface signature, exhibiting a coverage-limited kinetics according to a Langmuir mechanism. Due to the large variety of dimensions, porosities, and surface morphologies available in nature, these materials appear to be promising to improve the selectivity of gas sensing based on photoluminescence optochemical transduction.

Solid state dehalogenation of PCBs in contaminated soil using NaBH4.

In this work we present the results of an experimental study on the abatement of polychlorinated biphenyl (PCBs) in contaminated soil using a high energy milling technique, that promotes a reaction only by impact between milling bodies. A sample of soil from a controlled landfill was treated with powdered NaBH(4) using two different hydride/soil ratios (5 and 2.5% w/w). The efficiency of the dehalogenation/hydrogenation reaction was studied as a function of the milling time (3.5 up to 30 h). After each run, the total PCBs content and the production of inorganic chloride were measured. The complete abatement was obtained with a starting PCBs concentration of about 2600 mg/kg. The residual PCBs concentration resulted to be <0.2 mg/kg. The final products of the treatment were biphenyl and NaCl. Other toxic or hazardous organic by-products were not generated. Boron was found as boric acid.

Porous silicon microcavities for optical hydrocarbons detection

Large, repeatable and selective red-shifts in the reflectivity spectra of porous silicon microcavities (PSMs) have been registered after exposure to vapour or dip into liquid aliphatic hydrocarbons with different molecular weights. The shift in the resonant peak of the optical microcavity is due to average refractive index changes, induced by capillary condensation of the organic species into the silicon pores.

Hybrid polymer-porous silicon photonic crystals for optical sensing

Porous silicon based one-dimensional photonic crystals, such as Bragg mirror and optical microcavity, has been modified by infiltration of a new biocompatible polymer, an amino functionalized poly(e-caprolactone) (PCL-NH2), and characterized as optical biochemical sensors. The entrapped polymer adds strong chemical stability to the nanocrystalline matrix on exposure to alkaline solutions preserving the device sensing abilities in monitoring volatile substances and chemical compounds. On the basis of these results, these hybrid structures can be proposed as a high performance platform for biochemical applications.

Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus

A crystalline silicon surface can be made biocompatible and chemically stable by a self-assembled biofilm of proteins, the hydrophobins (HFBs) purified from the fungus Pleurotus ostreatus. The protein-modified silicon surface shows an improvement in wettability and is suitable for immobilization of other proteins. Two different proteins were successfully immobilized on the HFBs-coated chips: the bovine serum albumin and an enzyme, a laccase, which retains its catalytic activity even when bound on the chip. Variable-angle spectroscopic ellipsometry (VASE), water contact angle (WCA), and fluorescence measurements demonstrated that the proposed approach in silicon surface bioactivation is a feasible strategy for the fabrication of a new class of hybrid devices.

Optical characterization of liquid crystals by combined ellipsometry and half-leaky-guided-mode spectroscopy in the visible-near infrared range

In this paper, we present our results for the anisotropic refractive index measurements of commonly used liquid crystal (LC) materials (E7 and 5CB) in the whole visible–near infrared range. In order to achieve a high accuracy in the obtained values, we have employed a combination of two techniques, namely, Mueller matrix spectroscopic ellipsometry in transmission and half-leaky-guided-mode (HLGM). Measurements with the HLGM technique are usually performed at a single wavelength. In order to obtain a spectroscopic measurement based on the HLGM technique in a wide wavelength range, we modified our commercial VASE® ellipsometer. In particular, we designed a sample holder by means of which measurements in guiding structures are also possible. Thus, we take advantage of the wide-spectrum light, emitted from a standard Xe lamp of the ellipsometer, also in the HLGM setup. The complementary and in-one-setup utilization of both techniques has allowed us to overcome most of the problems previously encountered in ap...

Preparation of activated carbons from heavy-oil fly ashes

The use of heavy oil fly ash with high ash content (45 wt.%) as a precursor for the preparation of activated carbons has been investigated. The raw fly ash and the fly ash with lower ash content, obtained by a HCl/HF washing treatment, have been pyrolyzed at 900 degrees C and then activated with CO(2) in the temperature range of 800-900 degrees C for different times. The activated carbons have been characterised as regards the surface area and the pore volume. The evolution of the porosity has been related to the burn-off degree.

Biologically enabled sub-diffractive focusing

Evolution shows that photonic structures are a constituent part of many animals and flora. These elements produce structural color and are useful in predator-prey interactions between animals and in the exploitation of light for photosynthetic organisms. In particular, diatoms have evolved patterned hydrated silica external valves able to confine light with extraordinary efficiency. Their evolution was probably guided by the necessity to survive in harsh conditions of sunlight deprivation. Here, we exploit such diatom valves, in conjunction with structured illumination, to realize a biological super-resolving lens to achieve sub-diffractive focusing in the far field. More precisely, we consider a single diatom valve of Arachnoidiscus genus which shows symmetries and fine features. By characterizing and using the transmission properties of this valve using the optical eigenmode technique, we are able to confine light to a tiny spot with unprecedented precision in terms of resolution limit ratio, corresponding in this case to 0.21λ/NA.

High accuracy optical characterization of anisotropic liquids by merging standard techniques

The design and fabrication of active and passive hybrid photonic devices, with tunable optical properties, based on liquid crystals require a very accurate knowledge of their anisotropic refractive indices up to 100ppm in the wavelength region of interest. At this aim, the authors have integrated two standard optical techniques commonly used in liquid crystals characterization, the variable angle spectroscopic ellipsometry and the half leaky guided mode spectroscopy, exploiting their best performances and overcoming their limits. The dispersion curves of nematic liquid crystal E7 have been estimated in the 450–1700nm wavelength interval with both precision and accuracy of 10−4.