L. L. P. Vrijmoed

A novel N-cinnamoylcyclopeptide containing an allenic ether from the fungus Xylaria sp. (strain #2508) from the South China Sea

Abstract A novel cyclic peptide containing an allenic ether of a N-(p-hydroxycinnamoyl)amide, and two aromatic allenic ethers were isolated from the endothytic fungus Xylaria sp. from the South China Sea. Their structures were determined by analysis of spectroscopic data, mainly 2D NMR experiments.

Two new metabolites of a marine endophytic fungus (No. 1893) from an estuarine mangrove on the South China Sea coast

Abstract The endophytic fungus No. 1893 was isolated from the dropper of Kandelia candel from an estuarine mangrove on the South China Sea coast. The ethyl acetate extract of the fermentation broth of this fungus strain exhibited cytotoxicity toward NCI4460 and Bel-7402, and high activities against Heliothis armigera (Huhner) and Sinergasilus spp. Two new lactones, 1893A ( 1 ) and B ( 2 ), together with 5-(p-hydroxybenzyl)hydantoin and two cyclodipeptides, cyclo-(Ser-Leu) and cyclo-(Phe-Gly), were isolated from the extract. Their structures were determined by spectroscopic experiments, including X-ray diffraction.

Eicosapentaenoic and docosahexaenoic acids production by and okara-utilizing potential of thraustochytrids.

Nine thraustochytrid strains isolated from subtropical mangroves were screened for their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production potential in a glucose yeast extract medium. Their ability to utilize okara (soymilk residue) for growth and EPA and DHA production was also evaluated. EPA yield was low in most strains, while DHA level was high on glucose yeast extract medium, producing 28.1–41.1% of total fatty acids, for all strains, with the exception of Ulkenia sp. KF13. The DHA yield of Schizochytrium mangrovei strains ranged from 747.7 to 2778.9 mg/l after 52 h of fermentation at 25°C. All strains utilized okara as a substrate for growth, but DHA yield was lower when compared with fermentation in a glucose yeast extract medium. Journal of Industrial Microbiology & Biotechnology (2001) 27, 199–202.

The metabolites of the mangrove fungus Verruculina enalia No. 2606 from a salt lake in the Bahamas

Two metabolites enalin A (1) and B (2), together with hydroxymethyl furfural (3) and three cyclodipeptides (4, 5 and 6), were isolated from the mangrove fungus Verruculina enaria from a salt lake in the Bahamas. Their structures were determined by spectroscopic methods, mainly by 2D NMR spectroscopic analyses. A possible biosynthetic scheme to 1 and 2 is presented.

Benzofuran Derivatives from the Mangrove Endophytic Fungus Xylaria sp. (#2508)

Three metabolites, named xyloketal J (1), xyloester A (2), and xyloallenolide B (3), together with the known substituted dihydrobenzofuran (4) were isolated from the mangrove endophytic fungus Xylaria sp. (#2508). Structures were determined by spectroscopic methods, mainly 1D and 2D NMR.

Penicillazine, a Unique Quinolone Derivative with 4 H -5,6-Dihydro-1,2-oxazine Ring System from the Marine Fungus Penicillium sp. (Strain #386) from the South China Sea

Abstract Penicillazine (1) is a new compound with both quinolone and 4H-5,6-dihydro-1,2-oxazine ring systems that was isolated from a culture of the marine fungus Penicillium sp. (strain #386). Its structure was elucidated using spectroscopic methods, primarily 2D NMR techniques, and was confirmed by an X-ray diffraction analysis. Variations in the 1H NMR spectrum of penicillazine (1) were observed over a 65°C temperature range.

Xyloketal G, a novel metabolite from the mangrove fungus Xylaria sp. 2508

A novel metabolite 1, named xyloketal G, was isolated from cultures of marine derived mangrove fungus Xylaria sp. 2508. Its structure was elucidated by analysis of spectroscopic data.

Extracellular cellulolytic enzyme profiles of five lignicolous mangrove fungi

Five lignicolous mangrove fungi (Hypoxlon oceanicum, Julella avicenniae, Lignincola laevis, Savoryella lignicola and Trematosphaeria mangrovei) produced extracellular endoglucanase, cellobiohydrolase and β-glucosidase when cultivated under static conditions in a defined liquid growth medium. Agitation of cultures completely repressed enzyme production. Cellulase production by H. oceanicum was 2–5 fold higher compared to other strains. Increasing salinity generally resulted in reduced enzyme production although responses varied, but J. avicenniae appeared relatively unaffected by salinity within the range 0–3.4%.

A new isochroman from the marine endophytic fungus 1893

A new isochroman, 6-hydroxy-3-methylisochroman-5-carboxylic acid, and six known compounds were isolated from the culture of marine-derived mangrove fungus 1893#. Their structures were elucidated by analysis of spectroscopic data.

1H and 13C NMR assignments for 6‐demethylvermistatin and two penicillide derivatives from the mangrove fungus Guignardia sp. (No. 4382) from the South China Sea

One new compound 6‐demethylvermistatin (1), together with two known compounds, the penicillide derivatives (2) and (3) were isolated from the mangrove fungus Guignardia sp. No. 4382 obtained from the South China Sea. Their structures were assigned using high‐resolution electron ionization mass spectrometry(HREIMS), 1H and 13C NMR spectra, DEPT, and by 2D COSY, HMQC, and HMBC experiments. The absolute configuration of 1 was established by comparison of its CD with that of vermistatin. Copyright