L. R. Coelho

Identificação sorológica e relação filogenética de Salmonella spp. de origem suína

Salmonella spp. is an important zoonotic pathogen that can spread along the production chain of swines. The objective was to evaluate the incidence of Salmonella spp. in feces of swines in termination phase in the farm, in the pre-slaughter and environmental samples, identify the serotypes and establish a phylogenetic relationship among the isolates. Three collections were done in different batches of pigs housed in the termination pen and in the same animals after transport to the slaughterhouse totaling 90 plots and 9 environmental samples. The transport does not influenced the percentage of isolation of the microorganism (p>0.05). Of the total of 99 samples, 50 (50.5%) were identified as Salmonella spp., and was identified a variety of serovars: Agona (30%), Typhimurium (26%), Minnesota (24%), Infantis (18%) and Panama (2%). Dendrograms showed homology among isolates of different serovars grouped into clusters. The similarity was independent of the local of isolation, indicating the presence of several clones. The main sources of infection were cross-contamination between animals and environment and the consumption of contaminated feed. The diversity of strains and homology among the isolates indicates a common origin, demonstrating a need for monitoring of zoonotic bacterias and the deployment of more effective control measures for Salmonella spp. in swines.

Campylobacter jejuni in commercial eggs

This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.

Avaliação in vitro de óleos essenciais na inibição de Staphylococcus aureus e Escherichia coli

This work was carried through with the objective to evaluate the effect in vitro of essential oils in the inhibition of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. We tested the essential oils of rosemary (Rosmarinus officinalis), marjoran (Origanum marjorana L.), basil (Ocimum basilicum L.), eucalyptus (Eucalyptus citriodora), citronela (Cymbopogon nardus), grass-lemon (Cymbopogon citratus), previously extracted for the technique of drag the vapor. The oils had been submitted at 10 different dilutions (1/9, 2/8, 3/7, 4/6, 5/5, 6/4, 7/3, 8/2, 1/9, 1). The used technique was the embodiment of the samples in barren absorbent records that had been added on agar to contain the microorganisms and reading to subsequent of inhibition halos. The results had demonstrated that all the essential oils in the tested concentrations had not presented innibitory characteristics front to the Escherichia aureus coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Campylobacter em frangos no Brasil – Revisão de literatura

Campylobacter sp. is recognized worldwide as the bacterium that causes more diarrhea in humans, being reported as the most common zoonotic infection. Poultry meat and its derivatives are the most incriminated in the transmission to humans. The need for methods faster and less laborious detection has led to significant advances in research development and commercialization of diagnostic kits with high sensitivity and specificity in the world. Only with the knowledge of the different factors that influence the presence and multiplication of the microorganism, it is possible to establish preventive policies to prevent or control it. Thus, it is necessary to monitor diagnostic techniques are reproducible so safely subsidize the different actions for its control in Brazil.