Priscila Christen Nalevaiko

Characterization of the virulence, growth temperature and antibiotic resistance of the Campylobacter jejuni IAL 2383 strain isolated from humans

The objective of this study was to characterize the C. jejuni IAL2383 strain isolated from humans in Brazil. Transcripts for the racR, dnaJ and ciaB genes were found and flaA, plda and cadF genes were present in the genome and bacteria was sensitive to most of the important antimicrobials used to treat humans. C. jejuni IAL2383 is a good experimental model to analyze the interactions with cells.

Campylobacter jejuni in commercial eggs

This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.

Feed can be a source of Campylobacter jejuni infection in broilers

ABSTRACT 1. The aim was to determine the importance of a contaminated diet as a possible cause of Campylobacter jejuni infection in broilers. 2. This study evaluated the viability of C. jejuni in both starter and finisher diets and the interference from other mesophilic bacteria in this viability. 3. Starter and finisher samples of broiler diet were deliberately contaminated with 3 or 5 log CFU·g−1 of C. jejuni (NCTC 11351) and then maintained at two different storage temperatures (25°C or 37°C) for 3 or 5 d. 4. C. jejuni survived during this period and, when inoculated at 103 CFU·g−1, multiplied with greater proliferation at a storage temperature of 37°C. There was no relationship between the amount of mesophilic bacteria and C. jejuni viability. 5. This study highlights the importance of the diet in the epidemiology of C. jejuni in broilers.

Apostila ilustrada de cirurgia veterinária

This material is an illustrated textbook of surgery that has the function to offer consultation support to the learner of Veterinary Surgery, addressing the topics: Instrumentalization Surgical, Suture Materials, Surgical General Principles, Surgical Incisions of the Abdominal Wall and Sutures Used in Veterinary Medicine.

Avaliação in vitro de óleos essenciais na inibição de Staphylococcus aureus e Escherichia coli

This work was carried through with the objective to evaluate the effect in vitro of essential oils in the inhibition of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. We tested the essential oils of rosemary (Rosmarinus officinalis), marjoran (Origanum marjorana L.), basil (Ocimum basilicum L.), eucalyptus (Eucalyptus citriodora), citronela (Cymbopogon nardus), grass-lemon (Cymbopogon citratus), previously extracted for the technique of drag the vapor. The oils had been submitted at 10 different dilutions (1/9, 2/8, 3/7, 4/6, 5/5, 6/4, 7/3, 8/2, 1/9, 1). The used technique was the embodiment of the samples in barren absorbent records that had been added on agar to contain the microorganisms and reading to subsequent of inhibition halos. The results had demonstrated that all the essential oils in the tested concentrations had not presented innibitory characteristics front to the Escherichia aureus coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Campylobacter em frangos no Brasil – Revisão de literatura

Campylobacter sp. is recognized worldwide as the bacterium that causes more diarrhea in humans, being reported as the most common zoonotic infection. Poultry meat and its derivatives are the most incriminated in the transmission to humans. The need for methods faster and less laborious detection has led to significant advances in research development and commercialization of diagnostic kits with high sensitivity and specificity in the world. Only with the knowledge of the different factors that influence the presence and multiplication of the microorganism, it is possible to establish preventive policies to prevent or control it. Thus, it is necessary to monitor diagnostic techniques are reproducible so safely subsidize the different actions for its control in Brazil.