Laerte Pereira de Almeida

Anemia ferropriva em crianças de 6 a 12 meses atendidas na rede pública de saúde do município de Viçosa, Minas Gerais

This study aimed at verifying the prevalence of anemia in 6 to 12-month-old infantsattended at the public health service of the city of Vicosa, state of Minas Gerais, and analyzing some possible risk factors. Information was obtained through questionnaire applied to those responsible for the infants and through the verification of the anthropometric measures and the hemoglobin dosage by portable hemoglobinometer. In diagnosing anemia, a 11g/dL cutoff point was used. The prevalence of iron deficiency anemia in a total of 204 infants reached 60.8%, and 55.6% of the cases of anemia were serious. The hemoglobin average was 9.28±1.07 mg/dL in anemic infants, and 12.07± 0.89 mg/dL in non-anemic ones. The fathers low school level and the mothers age showed statistical association with anemia (p<0.05). A high prevalence and severity of the anemia was found, emphasizing the need for prevention and control of this disease in the city of Vicosa.

Isolamento de Salmonella sp e Staphylococcus aureus no processo do abate suíno como subsídio ao sistema de Análise de Perigos e Pontos Críticos de Controle - APPCC

O presente estudo foi realizado para avaliar a presenca de Salmonella sp e o numero de Staphylococcus aureus na superficie de carcacas suinas e caracterizar os perigos microbiologicos em diferentes etapas do abate e pontos criticos de controle (PCCs), atraves da quantificacao de riscos (odds ratio). Um total de 120 esfregacos superficiais de carcaca suina foi coletado em um matadouro-frigorifico, apos o escaldamento/depilacao (ponto A), antes da evisceracao (B), apos evisceracao e serragem da carcaca (C) e apos 24 horas de refrigeracao (D). Salmonella sp foi encontrada com uma frequencia media de 11,7% (14) nas carcacas, enquanto o numero de S. aureus variou entre 1,2 e 1,5 log UFC/cm2 em 11,7% das carcacas amostradas, sem evidenciar diferenca estatistica entre os pontos A, B, C e D. Pode-se concluir que os riscos de contaminacao por Salmonella sp e S. aureus foram os mesmos nas etapas do abate de suinos consideradas neste estudo.

Biological Behaviour of Bonelike® Graft Implanted in the Tibia of Humans

This paper reports the ability of Bonelike® to regenerate bone defected areas when implanted in the tibia of 3 patients (average age of 59 years) during a high tibial osteotomy (HTO) to treat medial compartment osteoarthritis of the knee. Bonelike® is a synthetic bone graft designed to mimic the inorganic composition of bone using a patent process that consists of liquid sintering hydroxyapatite in the presence of CaO-P2O5 based glass. The preliminary clinical evaluation performed showed that an intimate contact between new formed bone and Bonelike® was established (67±10 %), with no fibrous interface. Therefore, Bonelike® is a single-handedly viable osteoconductive synthetic grafting material as it has been demonstrated from the profiles of radiological, histological and scanning electron microscopy analyses for the six months implantation period.

In Vitro Mineralisation of Human Bone Marrow Cells Cultured on Bonelike

Bonelike is a CaO-P2O5 based glass-reinforced hydroxyapatite (HA) designed to mimic the inorganic composition of the bone tissue. This work evaluates the response of human bone marrow cells to Bonelike  concerning cell proliferation and osteoblast differentiation. HA was used as control material. Results showed that Bonelike  allowed the proliferation of bone marrow cells and their complete differentiation, as evidenced by t he formation of cell-mediated mineralisation. In comparison with HA, Bonelike  had a positive effect on the expression of alkaline phosphatase and also on the formation of a mineralised matrix, two osteobla st markers. Introduction Bonelike is a synthetic hydroxyapatite (HA) that is sintered in the pr esence of CaO-P 2O5-based glasses using a patented process [1]. This synthetic bone graft w as designed to improve the mechanical properties of calcium phosphate ceramics and mimic the inorganic composition of bone tissue. The physicochemical and mechanical behaviour of Bonelike  hav been extensively reported in literature [1-3]. Previous in vitro biological studies showed that glass-reinforced HA composites al low the proliferation of MG63 osteoblast-like cells and human bone marrow ce lls and the expression of osteoblast markers [4-6]. Also, in vivo studies performed in a rabbit model demonstrated that Bonelike composites induced earlier new bone formation around implants than HA [7]. Recently, a composite prepared by the addition (4 wt %) of a glass with the composition of 65P2O5-15CaO-10CaF 2-10Na2O (in % mol) to HA was subject of clinical trials in implantol ogy and maxillofacial surgery [8]. This study demonstrated extensive new bone formation around implanted granules and continuous replacement by new bone. Osteoblasts a re the cells responsible for the formation of the bone tissue at the bone/material interface and the present work evaluates the response of human bone marrow cells to Bonelike  composite, with the same chemical composition, concerning cell proliferation and osteoblast differentiation. HA was used as control material. Key Engineering Materials Online: 2003-12-15 ISSN: 1662-9795, Vols. 254-256, pp 821-824 doi:10.4028/www.scientific.net/KEM.254-256.821