László Láng

Composition and End-Use Quality of 150 Wheat Lines Selected for the HEALTHGRAIN Diversity Screen

The HEALTHGRAIN program is focused on developing new healthy food products based on wholegrains of wheat and other cereals, by combining enhanced nutritional quality with good agronomic performance and processing quality. A sample set comprising 130 winter and 20 spring wheat varieties was therefore selected to identify the range of variation in a number of phytochemical and dietary fiber components. These lines were also analyzed for their technological properties (protein and gluten contents, Zeleny sedimentation, bran yield, kernel hardness, etc.), using samples grown on adjacent sites for two successive seasons (2004-2005, 2005-2006). On the basis of the frequency distribution and principal component analysis it was concluded that significant variation for technological quality traits is present in the 150 wheat lines and that it is possible to combine enhanced nutritional quality with good agronomic performance and processing properties.

Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality

An amaranth (Amaranthus hypochondriacus) albumin gene, encoding the 35-kDa AmA1 protein of the seed, with a high content of essential amino acids, was used in the biolistic transformation of bread wheat (Triticum aestivum L.) variety Cadenza. The transformation cassette carried the ama1 gene under the control of a powerful wheat endosperm-specific promoter (1Bx17 HMW-GS). Southern-blot analysis of T1 lines confirmed the integration of the foreign gene, while RT-PCR and Western-blot analyses of the samples confirmed the transcription and translation of the transgene. The effects of the extra albumin protein on the properties of flour, produced from bulked T2 seeds, were calculated using total protein and essential amino acid content analysis, polymeric/monomeric protein and HMW/LMW glutenin subunit ratio measurements. The results indicated that not only can essential amino acid content be increased, but some parameters associated with functional quality may also be improved because of the expression of the AmA1 protein.

Diversity of agronomic and morphological traits in a mutant population of bread wheat studied in the Healthgrain program

A mutant population of spring wheat cv. Cadenza was produced at Rothamsted Research in 2004–5, both for TILLING and to generate variation in the contents of phytochemical components studied in the Healthgrain program. The agronomic and morphological properties of this mutant hexaploid wheat population (generations M3–M6) were studied in a 3-year field experiment. Most of the traits were scored according to UPOV TG/3/11, namely the time of ear emergence, plant height, ear glaucosity, shape, density and length, presence of awns and scurs, seasonal type, and grain colour. Other characters such as visible mutant phenotypes, ear sterility, heterogeneity of head rows, leaf colour and responses to powdery mildew and leaf rust were also studied. Variation in certain breadmaking quality parameters was also studied. The EMS mutant Cadenza lines studied showed wide diversity in terms of morphological and agronomic properties. The variation in agronomic properties was lower in 2007 and 2008 than in 2006, partly because of the SSD (single seed descent) in the M4 generation and partly because of the loss of late heading genotypes. The diversity was lowest in 2007, probably due to the extremely dry weather.

Effect of heat and drought stress on the structure and composition of arabinoxylan and β-glucan in wheat grain

The effects of heat (H), drought (D) and H+D (from 12th day after heading for 15 days) on the dietary fiber content and composition (arabinoxylan (AX) and β-glucan) of three winter wheat varieties (Plainsman V, Mv Magma and Fatima 2) were determined. Results showed that H and D stress decreased the TKW, the β-glucan contents of the seeds and the quantity of the DP3+DP4 units, while the protein and AX contents increased. The highest amounts of AX and proteins were in the H+D stressed samples with heat stress also increasing the water extractability (WE) of the AX. However, while the content of AX content was generally increased by all stresses, drought stress had negative effect on the AX content of the drought tolerant Plainsman V. Fatima 2 behaved similarly to Plainsman V as regards to its drought tolerance, but was very sensitive to heat stress, while Mv Magma was the most resistant to heat stress.

Identification, Cloning and Characterisation of a HMW-Glutenin Gene from an Old Hungarian Wheat Variety, Bánkúti 1201

Despite its good functional properties, the varietyBánkúti 1201 has been found to possess 2 + 12 or 3 + 12 allelecomposition on chromosome 1D. In order to determine the reason for itsquality traits a gene-specific PCR technique was applied in preliminaryexperiments to examine the HMW glutenin allele composition of thevariety. In the course of the analysis a fragment characteristic ofBánkúti 1201 was identified and the nucleotide sequence wasdetermined. This showed the presence of a 1Ax2* gene variantwhich, despite near homology, differed from the original 1Ax2* geneat one important point. At 1181 bp of the 1Ax2* sequencenucleotide exchange was observed which is the middle nucleotide of theTCT–TGT base triplet, involving the exchange of serine for cysteine. Thegene was designated 1Ax2*B. The presence of an extrasulphydryl group, like that of the extra cysteine in the 1Dx5 gene,facilitates the formation of further disulphide bonds, might lead to animprovement in gluten quality characters.

Computerised Data Management System for Cereal Breeding

A program package for wheat breeding was compiled inMartonvásár in 1983 and was used for over 10 years with minormodifications to satisfy breeding requirements. Due to new ideas andadvances made in the field of computer science and othertechnologies the data structure and program package have been redesigned.The chief emphasis in developing the Martonvásár Wheat BreedingSoftware is on the handling of genealogical and observation data and on theclassifications required for well-based selection decisions. The widely-usedWindows 9x/NT operational system, the MSAccess data base-handlingsystem and the Visual Basic 5.0 programming language have been chosenfor this purpose. The subprograms controlling major breeding tasks includethe transfer of the data of the selected lines, with the necessarymodifications, to the data base of the new experimental year, the handlingof new crosses and seed shipment data, the preparation of the field plan,various output (label, fieldbook) and input (manual, online) possibilities anda simple statistical module.

Breeding for breadmaking quality using old Hungarian wheat varieties

Due to their broad population diversity, old wheat varieties or landraces play an important role in increasing the genetic variability of agronomic traits. On these grounds, an analysis was made of the high molecular weight (HMW) glutenin subunit composition of the old Hungarian wheat variety Bankuti 1201. It was found that several genotypes with differing breadmaking qualities can be distinguished for this character. When using old varieties in breeding, it is possible to broaden the genetic background of characters responsible for breadmaking quality by separating the populations. A more detailed analysis of the protein composition of germplasm created in this way will be required to obtain a better understanding of this complex character for its conscious introduction into breeding programmes.

A retrospective analysis of HMW and LMW glutenin alleles of cultivars bred in Martonvásár, Hungary

The glutenin allele gene-pool, the distribution of the individual alleles on the 6 loci coding for glutenin subunits and their combinations were determined in a sample population containing 107 cultivars bred and grown in Martonvasar, Hungary at the Agricultural Research Institute of the Hungarian Academy of Sciences. The database is based on the results of three independent analytical procedures carried out using the traditional SDS-PAGE based allele identification, the state-of-art MALDI-TOF technology and the high throughput capillary electrophoresis based on the lab-on-a-chip technique. The usefulness of integrating the information on both HMW GS and LMW GS allelic composition for future genetic and technological improvement is discussed.

Informatical background of field experiments

Our objectives were to assess and rank 3 soil condition variants - root zone improving (RI), mulch leaving (NIL) and minimum disturbing (MD) - suitability to soil quality improvement and demonstrate the plant responses to different soil state and rind a possible relation between soil condition and plant growth under extreme climate. To rank the soil-plant interrelations twelve soil and plant factors were assessed; quality and depth of loosening state, surface protection by plants and stubble residues, soil aggregation, earthworm number, water supply in the root zone, tillage-induced C-flux, rooting depth of plants, yield, dry biomass and biomass recycling. Finally, seven fundamental requirements were selected to mitigate climate-stress and to improve plant resistance to the heat damage. A climate damage mitigation soil tillage strategy can establish a harmony between soil conservation and the demands of crop production.The results of a small-pot experiment of cadmium-loading (5 mgkg) with the addition of zinc (5 mgkg) carried out are introduced in this paper. For this, soil samples were taken from 4 sites of plough-land, grassland and forest land-use types. Accumulation characteristics of the two metals as well as their interaction with the soil properties were analysed. The results show that land-use has significant effect on zinc uptake. There is close correlation between the zinc and cadmium uptake of plants as well as that cadmium concentration of soils plays an important role in it. Zinc shows positive correlation while cadmium shows negative correlation with organic matter. Beside organic colloids, inorganic colloids i.e. clay fraction also showed close correlation.The effects of soil and climatic properties was studied on the winter wheat (Triticum aestivum L.) concerning on the heavy metal content of the plants. During this study the distribution of cobalt, copper, iron, manganese, nickel, and zinc was studied within a plant. Sampling was carried out in the same period of subsequent three years between 1995 and 1997. Our hypothesis was that the individuals of the same species has similar physiological answer for the same environmental properties. In this study we found no significant differences among the different plant’s parts and years. Our results can reveal that the ecological indication of plants could be major driver of agro-environmental studies to recognize unusual patterns of soil attributes.

Cultivar identification by molecular markers

A total of 96 winter wheat (Triticum aestivum L.) cultivars registered in Hungary were analysed using 15 wheat microsatellite markers located on different chromosome arms. Analyses revealed 91 SSR alleles with sizes ranging from 123–239 base pairs. The total number of alleles per locus ranged from 2 (Gwm664 and Gwm415) to 11 (Gwm219) with an average number of 6.1. The polymorphic information content (PIC) values ranged from 0.06 to 0.85 with an average number of 0.60 for all markers. Several markers included allele sizes characteristic of a single or a small number of cultivars. At most 9 SSR markers were required to distinguish the 96 cultivars, so the simple sequence repeats could serve as a relatively cheap, rapid method for identifying winter wheat cultivars.