Laura K. Young

CANARY: the on-sky NGS/LGS MOAO demonstrator for EAGLE

EAGLE is a multi-object 3D spectroscopy instrument currently under design for the 42-metre European Extremely Large Telescope (E-ELT). Precise requirements are still being developed, but it is clear that EAGLE will require (~100 x 100 actuator) adaptive optics correction of ~20 - 60 spectroscopic subfields distributed across a ~5 arcminute diameter field of view. It is very likely that LGS will be required to provide wavefront sensing with the necessary sky coverage. Two alternative adaptive optics implementations are being considered, one of which is Multi-Object Adaptive Optics (MOAO). In this scheme, wavefront tomography is performed using a set of LGS and NGS in either a completely open-loop manner, or in a configuration that is only closed-loop with respect to only one DM, probably the adaptive M4 of the E-ELT. The fine wavefront correction required for each subfield is then applied in a completely open-loop fashion by independent DMs within each separate optical relay. The novelty of this scheme is such that on-sky demonstration is required prior to final construction of an E-ELT instrument. The CANARY project will implement a single channel of an MOAO system on the 4.2m William Herschel Telescope. This will be a comprehensive demonstration, which will be phased to include pure NGS, low-order NGS-LGS and high-order woofer-tweeter NGS-LGS configurations. The LGSs used for these demonstrations will be Rayleigh systems, where the variable range-gate height and extension can be used to simulate many of the LGS effects on the E-ELT. We describe the requirements for the various phases of MOAO demonstration, the corresponding CANARY configurations and capabilities and the current conceptual designs of the various subsystems.

Deformable mirror model for open-loop adaptive optics using multivariate adaptive regression splines

Open-loop adaptive optics is a technique in which the turbulent wavefront is measured before it hits the deformable mirror for correction. We present a technique to model a deformable mirror working in open-loop based on multivariate adaptive regression splines (MARS), a non-parametric regression technique. The models input is the wavefront correction to apply to the mirror and its output is the set of voltages to shape the mirror. We performed experiments with an electrostrictive deformable mirror, achieving positioning errors of the order of 1.2% RMS of the peak-to-peak wavefront excursion. The technique does not depend on the physical parameters of the device; therefore it may be included in the control scheme of any type of deformable mirror.

Vision science and adaptive optics, the state of the field.

Adaptive optics is a relatively new field, yet it is spreading rapidly and allows new questions to be asked about how the visual system is organized. The editors of this feature issue have posed a series of question to scientists involved in using adaptive optics in vision science. The questions are focused on three main areas. In the first we investigate the use of adaptive optics for psychophysical measurements of visual system function and for improving the optics of the eye. In the second, we look at the applications and impact of adaptive optics on retinal imaging and its promise for basic and applied research. In the third, we explore how adaptive optics is being used to improve our understanding of the neurophysiology of the visual system.

Not all aberrations are equal: reading impairment depends on aberration type and magnitude

The eyes optical components are imperfect and cause distortions in the retinal image that cannot be corrected completely by conventional spectacles. It is important to understand how these uncorrected aberrations (those excluding defocus and primary astigmatism) affect visual performance. We assessed reading performance using text with a simulated monochromatic aberration (defocus, coma, or secondary astigmatism), all of which typically occur in the normal population. We found that the rate of decline in reading performance with increasing aberration amplitude was smaller for coma than for secondary astigmatism or defocus. Defocus and secondary astigmatism clearly had an impact on word identification, as revealed by an analysis of a lexical frequency effect. The spatial form changes caused by these aberrations are particularly disruptive to letter identification, which in turn impacts word recognition and has consequences for further linguistic processing. Coma did not have a significant effect on word identification. We attribute reading impairment caused by coma to effects on saccade targeting, possibly due to changes in the spacings between letters. Effects on performance were not accompanied by a loss of comprehension confirming that even if an aberration is not severe enough to make text illegible it may still have a significant impact on reading.

Accounting for the phase, spatial frequency and orientation demands of the task improves metrics based on the visual Strehl ratio.

Advances in ophthalmic instrumentation have allowed high order aberrations to be measured in vivo. These measurements describe the distortions to a plane wavefront entering the eye, but not the effect they have on visual performance. One metric for predicting visual performance from a wavefront measurement uses the visual Strehl ratio, calculated in the optical transfer function (OTF) domain (VSOTF) (Thibos et al., 2004). We considered how well such a metric captures empirical measurements of the effects of defocus, coma and secondary astigmatism on letter identification and on reading. We show that predictions using the visual Strehl ratio can be significantly improved by weighting the OTF by the spatial frequency band that mediates letter identification and further improved by considering the orientation of phase and contrast changes imposed by the aberration. We additionally showed that these altered metrics compare well to a cross-correlation-based metric. We suggest a version of the visual Strehl ratio, VScombined, that incorporates primarily those phase disruptions and contrast changes that have been shown independently to affect object recognition processes. This metric compared well to VSOTF for letter identification and was the best predictor of reading performance, having a higher correlation with the data than either the VSOTF or cross-correlation-based metric.

Status update of the CANARY on-sky MOAO demonstrator

The CANARY on-sky MOAO demonstrator is being integrated in the laboratory and a status update about its various components is presented here. We also discuss the alignment and calibration procedures used to improve system performance and overall stability. CANARY will be commissioned at the William Herschel Telescope at the end of September 2010.

Different aberrations raise contrast thresholds for single-letter identification in line with their effect on cross-correlation- based confusability

We previously showed that different types of aberration defocus, coma, and secondary astigmatism affect reading performance via different mechanisms. In this paper, we show the contrary result that, for identification of isolated letters, the effects of rendering different types of aberration can be described by a single cross-correlation-based metric. Aberrations reduce the effective resolution of an optical system, quantified by the high-frequency fall-off of the modulation transfer function. They additionally cause spatial-frequency-dependent phase and contrast changes, which have a size-dependent effect on letter forms. We used contrast threshold as our performance measure, instead of distance acuity, to separate the effects of form alterations from those of resolution limits. This measure is additionally appropriate in comparing single-letter-based performance to reading at a fixed distance. The relationship between a cross-correlation-based measure of letter confusability and performance was the same for all three types of aberration. For reading, we had found a different relationship for coma than for defocus and secondary astigmatism. We conclude that even when two tasks--letter identification and reading--use the same component stimulus set, the combination of multiple letters in a reading task produces functional differences between the effects of these aberrations that are not present for isolated letters.

Deformable mirror controller for open-loop adaptive optics

A Deformable Mirror Controller (DMC) has been devised to overcome the open-loop nature of Multi Object Adaptive Optics (MOAO), in particular for AO systems with update rates of 1 ms or less. The system is based on a figure sensor, which uses a monochromatic illumination source and a Shack-Hartmann (SH) wavefront sensor (WFS) to obtain a fine sampling of DMs 3D surface. The sensors beam is optically separated from the science path in order to not interfere with science observations. The DMC incorporates a real-time controller in charge of driving the DM. This controller runs in a dedicated Field-Programmable-Gate-Array (FPGA) based processor to keep up with stringent speed requirements. The DMC is being tested in the laboratory and is part of CANARY, an MOAO on-sky demonstrator to be installed at the William Hershel Telescope.

Critical band masking reveals the effects of optical distortions on the channel mediating letter identification

There is evidence that letter identification is mediated by only a narrow band of spatial frequencies and that the center frequency of the neural channel thought to underlie this selectivity is related to the size of the letters. When letters are spatially filtered (at a fixed size) the channel tuning characteristics change according to the properties of the spatial filter (Majaj et al., 2002). Optical aberrations in the eye act to spatially filter the image formed on the retina—their effect is generally to attenuate high frequencies more than low frequencies but often in a non-monotonic way. We might expect the change in the spatial frequency spectrum caused by the aberration to predict the shift in channel tuning observed for aberrated letters. We show that this is not the case. We used critical-band masking to estimate channel-tuning in the presence of three types of aberration—defocus, coma and secondary astigmatism. We found that the maximum masking was shifted to lower frequencies in the presence of an aberration and that this result was not simply predicted by the spatial-frequency-dependent degradation in image quality, assessed via metrics that have previously been shown to correlate well with performance loss in the presence of an aberration. We show that if image quality effects are taken into account (using visual Strehl metrics), the neural channel required to model the data is shifted to lower frequencies compared to the control (no-aberration) condition. Additionally, we show that when spurious resolution (caused by π phase shifts in the optical transfer function) in the image is masked, the channel tuning properties for aberrated letters are affected, suggesting that there may be interference between visual channels. Even in the presence of simulated aberrations, whose properties change from trial-to-trial, observers exhibit flexibility in selecting the spatial frequencies that support letter identification.

Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish

The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light. We report dramatically improved resolution and speed of cell targeting in the zebrafish kidney through the use of a selective plane illumination microscope (SPIM). Furthermore, through the novel incorporation of a Bessel beam into the SPIM imaging arm, we were able to improve on targeting speed and precision. The low diffraction of the Bessel beam coupled with the ability to tightly focus it through a high NA lens allowed precise, rapid targeting of subsets of cells at anatomical depth in live, developing zebrafish kidneys. We demonstrate that these specific targeting strategies significantly increase the speed of optoablation as well as fish survival.