Leandro Silva Barbosa

Desenvolvimento de Nasonia vitripennis (Walker, 1836) (Hymenoptera: Pteromalidae) em pupas de Cochliomyia macellaria (Fabricius, 1775) (Diptera: Calliphoridae), utilizando diferentes densidades do parasitóide

The post-embryonic development, the productivity of the pupae, the rate of parasitism and the sexual ratio of Nasonia vitripennis (Walker) reared in pupae of Cochliomyia macellaria (Fabricius) were studied. Different densities of the parasitoid were used (proportions 1:1, 2:1, 3:1, 4:1, and 5:1) with exposition of 72 hours. Nulliparous females originating from the stock colony were individualized in test tubes covered with hydrophobic cotton and containing the host pupae. Each treatment was constituted by 10 repetitions. After the exposition, the wasps were discarded and the host pupae were individualized in test tubes until the emergency of the adults of C. macellaria or N. vitripennis. Samples of host pupae not exposed to parasitism and receiving the same experimental treatment were used as control. The post-embryonic period was significantly shorter in the proportion of 2:1 (13,73 days) and longer in the relation of 5:1. The peaks of emergence occurred in the 14th day after the exposition of the parasitoid to the host in all relations, except for the relation of 4:1(13rd day). The productivity of N. vitripennis does not vary significantly among the different proportions. As the number of parasitoids grows, its post-embryonic development gets slower and the sexual ratio of males grows up. The rate of parasitism showed a decrease when the host was exposed to five parasitoids.

Hemilucilia segmentaria (Fabricius, 1805) (Diptera: Calliphoridae) as new biological vector of eggs of Dermatobia hominis (Linnaeus Jr., 1781) (Diptera: Oestridae) in Reserva Biológica do Tinguá, Rio de Janeiro, Brazil

The aim of this note was to record for the first time the finding of Hemilucilia segmentaria acting as biological vector of Dermatobia hominis, during a study of the diversity of Calliphoridae at Reserva Biol gica do Tinguá, Rio de Janeiro, Brazil. The insects were captured using traps baited with chicken vicera, for a period of 28-30 h twice per month. In the period of one year, 1987 insects were collected, 7.5% of which belonged to the H. segmentaria; of these a female was captured in May 2001, carrying a mass of 20 eggs on the left side of its abdomen.

Diversity of yellow fever mosquito vectors in the Atlantic Forest of Rio de Janeiro, Brazil

INTRODUCTION Environmental modifications caused by human activities have led to changes in mosquito vector populations, and sylvatic species have adapted to breeding in urban areas. METHODS Mosquitoes were collected using ovitraps in three sampling sites in the Atlantic Forest in the State of Rio de Janeiro, Brazil. RESULTS We collected 2,162 Culicidae specimens. Haemagogus janthinomys and Haemagogus leucocelaenus, both sylvatic yellow fever virus vectors, were the most common species found. CONCLUSION There is a potential for the transmission of arboviruses in and around these natural reserves. Therefore, it is necessary to maintain entomological surveillance programs in the region.

Influência do aumento do número de pupas hospedeiras de Cochliomyia macellaria (Diptera, Calliphoridae) no desenvolvimento do parasitóide Nasonia vitripennis (Hymenoptera, Pteromalidae) em laboratório

The post-embryonic development, productivity, rate of parasitism and sexual ratio of Nasonia vitripennis (Walker, 1836) reared in pupae of Cochliomyia macellaria (Fabricius, 1775) were studied. Different densities of the host were used (proportion 1:1, 1:2, 1:3, 1:4, and 1:5) and the exposition in each one lasted 72 hours. Nulliparous females originating from the stock colony were individualized in test tubes covered with hydrophobic cotton and containing the host pupae. Each treatment was constituted by 10 repetitions. After the exposition, the wasps were discarded and the host pupae were individualized in test tubes until the emergence of the adults of C. macellaria or N. vitripennis. Samples of host pupae not exposed to parasitism and receiving the same experimental treatment were used as control. The parasitoids showed a slower development in relation to the increase of host density. There was a production decrease of parasitoids per host in elevated densities. The sexual ratio tended towards a deviation for the birth of females with the increase of the density of host. The rate of parasitism showed a decrease when the parasitoid was exposed to more than two host pupae.

Description of a new Kempnyia Klapálek from Brazil (Plecoptera: Perlidae) with life stages associated using DNA barcodes

Kempnyia couriae sp. nov. is described from specimens (male, female, and nymphs) collected in Rio de Janeiro State, southeastern Brazil. This species is distinguished from its congeners by the male penial armature, having an elongate gonopore, surpassing the hooks, hooks with penial apex forming a globular membranous structure, and by ventrally curved hooks. Females and a nymph were associated with males using DNA barcoding.

The classic episode of biological invasion: Cochliomyia macellaria (Fabricius, 1775) versus Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) - evaluation of the biotic potential

The classic episode of biological invasion: Cochliomyia macellaria (Fabricius, 1775) versus Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) - evaluation of the biotic potential. The biotic of the native fly Cochliomyia macellaria and the exotic Chrysomya megacephala was compared, through the reproductive capacity and longevity. The experiment took place in a climatized chamber, with four repetitions of 15 grouped couples of each species. The posture, egg mass weight, and death of individuals were observed daily. C. macellaria showed an average of longevity of 27.72 days while in C. megacephala the average was 41.96 days. The egg mass weight average per repetition was 0.58 g and 1.22 g, respectively. These results give a better understanding about the population dynamics of these species in nature.

Desempenho do parasitóide Nasonia vitripennis (Walker)(Hymenoptera, Pteromalidae) utilizando como hospedeiro Cochliomyia macellaria (Fabricius) (Diptera, Calliphoridae), sob diferentes tempos de exposição

The post-embryonic development, the productivity of the host pupae, the rate of parasitism and the sex ratio of Nasonia vitripennis reared in pupae of Cochliomyia macellaria were studied. Different exposition times were used: 24, 48, 72, 96 hours. Nulliparous females originating from the stock colony were individualized in test tubes covered with hydrophobic cotton and containing the host pupae. Each treatment was constituted by 10 replicates. After exposition, the hymenopterans were discarded and the host pupae were individualized in test tubes until the emergency of the adults of C. macellaria or N. vitripennis. Samples of host pupae not exposed to parasitism and receiving the same experimental treatment, were used as control. The parasitoids showed a slower development in relation to the increase exposition time. The 72 hours exposition was the one which enabled higher productivity. The sex ratio tended towards a deviation for the birth of females after 48 hour. The rate of parasitism increased up to 72 hours. The rate of dipterans emergence showed a decrease until 72 hours.

New species of Pseudoptilolepis Snyder, 1949 (Diptera: Muscidae) from Brazil

Pseudoptilolepis Snyder, 1949 (Diptera: Muscidae) is a Neotropical genus with 10 known species. The present paper describes a new species from Parque Nacional do Itatiaia (PNI). Male and female terminalia were dissected and illustrated. Key-Words. Atlantic Forest; Biodiversity; Morphology; Taxonomy.

Pupal case description of Systropus (Systropus) fumipennis Westwood, 1842 (Diptera: Bombyliidae: Toxophorinae: Systropodini)

The pupal case of Systropus (Systropus) fumipennis is described, illustrated and photographed for the first time. A pupae was found inside an unidentified Limacodidae coocon (Insecta: Lepidoptera), and a male adult emerged, allowing the species identification.